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Preparation of protein imprinted polymer beads by Pickering emulsion polymerization

Zhou, Tongchang LU ; Zhang, Ka LU ; Kamra, Tripta LU ; Bülow, Leif LU and Ye, Lei LU (2015) In Journal of Materials Chemistry. B 3(7). p.1254-1260
Abstract
We present a new method for preparation of protein-specific polymer beads based on surface molecular imprinting in Pickering emulsion. In the first step, adult human hemoglobin (Hb) was adsorbed on silica nanoparticles. The protein-coated silica particles were then used to stabilize an oil-in-water emulsion (Pickering emulsion) composed of cross-linking monomer in the oil phase. After free radical polymerization of the oil phase, the protein-silica particles were removed to leave Hb-imprinted sites on the polymer surface. The protein-imprinted polymer microspheres were characterized by scanning electron microscopy and their selectivity was investigated by protein binding analysis. The new synthetic method based on Pickering emulsion... (More)
We present a new method for preparation of protein-specific polymer beads based on surface molecular imprinting in Pickering emulsion. In the first step, adult human hemoglobin (Hb) was adsorbed on silica nanoparticles. The protein-coated silica particles were then used to stabilize an oil-in-water emulsion (Pickering emulsion) composed of cross-linking monomer in the oil phase. After free radical polymerization of the oil phase, the protein-silica particles were removed to leave Hb-imprinted sites on the polymer surface. The protein-imprinted polymer microspheres were characterized by scanning electron microscopy and their selectivity was investigated by protein binding analysis. The new synthetic method based on Pickering emulsion polymerization produced easily accessible Hb binding sites on the surface of spherical polymer particles, which are useful for protein separation, purification and analysis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Materials Chemistry. B
volume
3
issue
7
pages
1254 - 1260
publisher
Royal Society of Chemistry
external identifiers
  • wos:000349617600010
  • scopus:84929459850
ISSN
2050-7518
DOI
10.1039/c4tb01605j
language
English
LU publication?
yes
id
e913862b-0dc4-4d2e-9ffe-87da22128421 (old id 5168988)
date added to LUP
2015-03-25 11:08:31
date last changed
2017-09-24 03:19:33
@article{e913862b-0dc4-4d2e-9ffe-87da22128421,
  abstract     = {We present a new method for preparation of protein-specific polymer beads based on surface molecular imprinting in Pickering emulsion. In the first step, adult human hemoglobin (Hb) was adsorbed on silica nanoparticles. The protein-coated silica particles were then used to stabilize an oil-in-water emulsion (Pickering emulsion) composed of cross-linking monomer in the oil phase. After free radical polymerization of the oil phase, the protein-silica particles were removed to leave Hb-imprinted sites on the polymer surface. The protein-imprinted polymer microspheres were characterized by scanning electron microscopy and their selectivity was investigated by protein binding analysis. The new synthetic method based on Pickering emulsion polymerization produced easily accessible Hb binding sites on the surface of spherical polymer particles, which are useful for protein separation, purification and analysis.},
  author       = {Zhou, Tongchang and Zhang, Ka and Kamra, Tripta and Bülow, Leif and Ye, Lei},
  issn         = {2050-7518},
  language     = {eng},
  number       = {7},
  pages        = {1254--1260},
  publisher    = {Royal Society of Chemistry},
  series       = {Journal of Materials Chemistry. B},
  title        = {Preparation of protein imprinted polymer beads by Pickering emulsion polymerization},
  url          = {http://dx.doi.org/10.1039/c4tb01605j},
  volume       = {3},
  year         = {2015},
}