Resolving the hematopoietic stem cell state by linking functional and molecular assays
(2023) In Blood 142(6). p.543-552- Abstract
One of the most challenging aspects of stem cell research is the reliance on retrospective assays for ascribing function. This is especially problematic for hematopoietic stem cell (HSC) research in which the current functional assay that formally establishes its HSC identity involves long-term serial transplantation assays that necessitate the destruction of the initial cell state many months before knowing that it was, in fact, an HSC. In combination with the explosion of equally destructive single-cell molecular assays, the paradox facing researchers is how to determine the molecular state of a functional HSC when you cannot concomitantly assess its functional and molecular properties. In this review, we will give a historical... (More)
One of the most challenging aspects of stem cell research is the reliance on retrospective assays for ascribing function. This is especially problematic for hematopoietic stem cell (HSC) research in which the current functional assay that formally establishes its HSC identity involves long-term serial transplantation assays that necessitate the destruction of the initial cell state many months before knowing that it was, in fact, an HSC. In combination with the explosion of equally destructive single-cell molecular assays, the paradox facing researchers is how to determine the molecular state of a functional HSC when you cannot concomitantly assess its functional and molecular properties. In this review, we will give a historical overview of the functional and molecular assays in the field, identify new tools that combine molecular and functional readouts in populations of HSCs, and imagine the next generation of computational and molecular profiling tools that may help us better link cell function with molecular state.
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- author
- Jassinskaja, Maria LU ; Gonka, Monika and Kent, David G
- publishing date
- 2023-08-10
- type
- Contribution to journal
- publication status
- published
- keywords
- Retrospective Studies, Hematopoietic Stem Cell Transplantation, Hematopoietic Stem Cells, Cell Differentiation/physiology
- in
- Blood
- volume
- 142
- issue
- 6
- pages
- 543 - 552
- publisher
- American Society of Hematology
- external identifiers
-
- pmid:36735913
- scopus:85150302556
- ISSN
- 1528-0020
- DOI
- 10.1182/blood.2022017864
- language
- English
- LU publication?
- no
- additional info
- © 2023 by The American Society of Hematology. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved.
- id
- 517a5196-e5ec-4f22-825f-d45e519ec034
- date added to LUP
- 2025-01-16 16:05:49
- date last changed
- 2025-01-17 07:21:02
@article{517a5196-e5ec-4f22-825f-d45e519ec034,
abstract = {{<p>One of the most challenging aspects of stem cell research is the reliance on retrospective assays for ascribing function. This is especially problematic for hematopoietic stem cell (HSC) research in which the current functional assay that formally establishes its HSC identity involves long-term serial transplantation assays that necessitate the destruction of the initial cell state many months before knowing that it was, in fact, an HSC. In combination with the explosion of equally destructive single-cell molecular assays, the paradox facing researchers is how to determine the molecular state of a functional HSC when you cannot concomitantly assess its functional and molecular properties. In this review, we will give a historical overview of the functional and molecular assays in the field, identify new tools that combine molecular and functional readouts in populations of HSCs, and imagine the next generation of computational and molecular profiling tools that may help us better link cell function with molecular state.</p>}},
author = {{Jassinskaja, Maria and Gonka, Monika and Kent, David G}},
issn = {{1528-0020}},
keywords = {{Retrospective Studies; Hematopoietic Stem Cell Transplantation; Hematopoietic Stem Cells; Cell Differentiation/physiology}},
language = {{eng}},
month = {{08}},
number = {{6}},
pages = {{543--552}},
publisher = {{American Society of Hematology}},
series = {{Blood}},
title = {{Resolving the hematopoietic stem cell state by linking functional and molecular assays}},
url = {{http://dx.doi.org/10.1182/blood.2022017864}},
doi = {{10.1182/blood.2022017864}},
volume = {{142}},
year = {{2023}},
}