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The dynamics of insulin release from mouse pancreatic islet cells in suspension

Idahl, Lars åke ; Lernmark, Åke LU ; Sehlin, Janove and Täljedal, Inge Bert (1976) In Pflügers Archiv European Journal of Physiology 366(2-3). p.185-188
Abstract

The overall dynamics of glucose-induced insulin release was strikingly similar in dispersed cells and intact islets perifused in parallel. Both preparations exhibited a latency of 1-2 min, after which period there was a brisk rise of insulin release followed by a sustained second phase. During the second phase, insulin release from dispersed cells attained a stable plateau rate, whereas the release from intact islets continued to rise. Epinephrine (1 μM) inhibited the release in both preparations, but the return to basal rate was faster in the dispersed cells than in the intact islets. The dispersed cells oxidized glucose at a constant rate for at least 60 min; the glucose oxidation was markedly sensitive to changes of the glucose... (More)

The overall dynamics of glucose-induced insulin release was strikingly similar in dispersed cells and intact islets perifused in parallel. Both preparations exhibited a latency of 1-2 min, after which period there was a brisk rise of insulin release followed by a sustained second phase. During the second phase, insulin release from dispersed cells attained a stable plateau rate, whereas the release from intact islets continued to rise. Epinephrine (1 μM) inhibited the release in both preparations, but the return to basal rate was faster in the dispersed cells than in the intact islets. The dispersed cells oxidized glucose at a constant rate for at least 60 min; the glucose oxidation was markedly sensitive to changes of the glucose concentration in the range of 3-20 mM.

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author
; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
β-Cells in suspension, Glucose oxidation, Insulin release, Intact pancreatic islets, Pancreatic β-cells
in
Pflügers Archiv European Journal of Physiology
volume
366
issue
2-3
pages
185 - 188
publisher
Springer
external identifiers
  • pmid:186754
  • scopus:0017097090
ISSN
0031-6768
DOI
10.1007/BF00585876
language
English
LU publication?
no
id
517e83ee-3431-45b9-a83a-8c4ac48f64d2
date added to LUP
2019-09-18 12:12:58
date last changed
2020-01-20 15:27:10
@article{517e83ee-3431-45b9-a83a-8c4ac48f64d2,
  abstract     = {<p>The overall dynamics of glucose-induced insulin release was strikingly similar in dispersed cells and intact islets perifused in parallel. Both preparations exhibited a latency of 1-2 min, after which period there was a brisk rise of insulin release followed by a sustained second phase. During the second phase, insulin release from dispersed cells attained a stable plateau rate, whereas the release from intact islets continued to rise. Epinephrine (1 μM) inhibited the release in both preparations, but the return to basal rate was faster in the dispersed cells than in the intact islets. The dispersed cells oxidized glucose at a constant rate for at least 60 min; the glucose oxidation was markedly sensitive to changes of the glucose concentration in the range of 3-20 mM.</p>},
  author       = {Idahl, Lars åke and Lernmark, Åke and Sehlin, Janove and Täljedal, Inge Bert},
  issn         = {0031-6768},
  language     = {eng},
  month        = {01},
  number       = {2-3},
  pages        = {185--188},
  publisher    = {Springer},
  series       = {Pflügers Archiv European Journal of Physiology},
  title        = {The dynamics of insulin release from mouse pancreatic islet cells in suspension},
  url          = {http://dx.doi.org/10.1007/BF00585876},
  doi          = {10.1007/BF00585876},
  volume       = {366},
  year         = {1976},
}