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Characterization of the substitution pattern of cellulose derivatives using carbohydrate-binding modules

von Schantz, Laura LU ; Schagerlöf, Herje LU ; Nordberg Karlsson, Eva LU orcid and Ohlin, Mats (2014) In BMC Biotechnology 14.
Abstract
Background: Derivatized celluloses, such as methylcellulose (MC) and hydroxypropyl methylcellulose (HPMC), are of pharmaceutical importance and extensively employed in tablet matrices. Each batch of derivatized cellulose is thoroughly characterized before utilized in tablet formulations as batch-to-batch differences can affect drug release. The substitution pattern of the derivatized cellulose polymers, i.e. the mode on which the substituent groups are dispersed along the cellulose backbone, can vary from batch-to-batch and is a factor that can influence drug release. Results: In the present study an analytical approach for the characterization of the substitution pattern of derivatized celluloses is presented, which is based on the use of... (More)
Background: Derivatized celluloses, such as methylcellulose (MC) and hydroxypropyl methylcellulose (HPMC), are of pharmaceutical importance and extensively employed in tablet matrices. Each batch of derivatized cellulose is thoroughly characterized before utilized in tablet formulations as batch-to-batch differences can affect drug release. The substitution pattern of the derivatized cellulose polymers, i.e. the mode on which the substituent groups are dispersed along the cellulose backbone, can vary from batch-to-batch and is a factor that can influence drug release. Results: In the present study an analytical approach for the characterization of the substitution pattern of derivatized celluloses is presented, which is based on the use of carbohydrate-binding modules (CBMs) and affinity electrophoresis. CBM4-2 from Rhodothermus marinus xylanase 10A is capable of distinguishing between batches of derivatized cellulose with different substitution patterns. This is demonstrated by a higher migration retardation of the CBM in acrylamide gels containing batches of MC and HPMC with a more heterogeneous distribution pattern. Conclusions: We conclude that CBMs have the potential to characterize the substitution pattern of cellulose derivatives and anticipate that with use of CBMs with a very selective recognition capacity it will be possible to more extensively characterize and standardize important carbohydrates used for instance in tablet formulation. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Substitution pattern, Methylcellulose, Hydroxypropyl methylcellulose, Application of carbohydrate-binding modules
in
BMC Biotechnology
volume
14
article number
113
publisher
BioMed Central (BMC)
external identifiers
  • wos:000349858100001
  • scopus:84928668051
  • pmid:25540113
ISSN
1472-6750
DOI
10.1186/s12896-014-0113-9
project
Designed carbohydrate binding modules and molecular probes
language
English
LU publication?
yes
id
9230a2ed-054b-4534-8f32-bb18676d15d4 (old id 5180114)
date added to LUP
2016-04-01 14:21:37
date last changed
2021-09-22 04:19:12
@article{9230a2ed-054b-4534-8f32-bb18676d15d4,
  abstract     = {Background: Derivatized celluloses, such as methylcellulose (MC) and hydroxypropyl methylcellulose (HPMC), are of pharmaceutical importance and extensively employed in tablet matrices. Each batch of derivatized cellulose is thoroughly characterized before utilized in tablet formulations as batch-to-batch differences can affect drug release. The substitution pattern of the derivatized cellulose polymers, i.e. the mode on which the substituent groups are dispersed along the cellulose backbone, can vary from batch-to-batch and is a factor that can influence drug release. Results: In the present study an analytical approach for the characterization of the substitution pattern of derivatized celluloses is presented, which is based on the use of carbohydrate-binding modules (CBMs) and affinity electrophoresis. CBM4-2 from Rhodothermus marinus xylanase 10A is capable of distinguishing between batches of derivatized cellulose with different substitution patterns. This is demonstrated by a higher migration retardation of the CBM in acrylamide gels containing batches of MC and HPMC with a more heterogeneous distribution pattern. Conclusions: We conclude that CBMs have the potential to characterize the substitution pattern of cellulose derivatives and anticipate that with use of CBMs with a very selective recognition capacity it will be possible to more extensively characterize and standardize important carbohydrates used for instance in tablet formulation.},
  author       = {von Schantz, Laura and Schagerlöf, Herje and Nordberg Karlsson, Eva and Ohlin, Mats},
  issn         = {1472-6750},
  language     = {eng},
  publisher    = {BioMed Central (BMC)},
  series       = {BMC Biotechnology},
  title        = {Characterization of the substitution pattern of cellulose derivatives using carbohydrate-binding modules},
  url          = {https://lup.lub.lu.se/search/files/8182108/5268457.pdf},
  doi          = {10.1186/s12896-014-0113-9},
  volume       = {14},
  year         = {2014},
}