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The quest for ingested peanut protein in human serum

Pahlow Mose, Anja LU ; Mortz, Ejvind ; Stahl Skov, Per ; Mortz, Charlotte Gotthard ; Eller, Esben ; Sprogøe, Ulrik ; Barington, Torben and Bindslev-Jensen, Carsten (2020) In Allergy 75(7). p.1721-1729
Abstract

BACKGROUND: There is mounting evidence that systemic uptake of food allergens is key to triggering anaphylaxis. However, direct proof for this theory is still lacking. The purpose of this study was to quantify the absorption and to determine the absorption kinetics of immunoreactive peanut protein in relation to the allergic response in human.

METHODS: Quantitative protein assays including mass spectrometry, dot blots and Western blotting were developed to determine the level of Ara h 2 absorption in human serum. The double monoclonal sandwich ELISA was applied to quantify absorbed Ara h 2 and 6, and the basophil histamine release assay and the human passive cutaneous anaphylaxis test were utilized to study the absorption kinetics... (More)

BACKGROUND: There is mounting evidence that systemic uptake of food allergens is key to triggering anaphylaxis. However, direct proof for this theory is still lacking. The purpose of this study was to quantify the absorption and to determine the absorption kinetics of immunoreactive peanut protein in relation to the allergic response in human.

METHODS: Quantitative protein assays including mass spectrometry, dot blots and Western blotting were developed to determine the level of Ara h 2 absorption in human serum. The double monoclonal sandwich ELISA was applied to quantify absorbed Ara h 2 and 6, and the basophil histamine release assay and the human passive cutaneous anaphylaxis test were utilized to study the absorption kinetics of immunologically intact peanut proteins.

RESULTS: The protein assays worked but were not sensitive enough to trace the minute amounts of absorbed Ara h 2 in human serum. The level of Ara h 6 in serum was found to be up to 0.2 ng/mL, but Ara h 2 could not be detected with the ELISA. Both the in vivo and the in vitro methods were successful in demonstrating that: immunoreactive peanut protein was absorbed shortly after ingestion (≤5 minutes); the peanut protein concentration peaks between 1 and 4 hours; and peanut proteins can circulate for at least 48 hours in the bloodstream.

CONCLUSION: Ingested peanut protein is absorbed systemically and retains its immunoreactive capacity in human serum. However, the precise quantities and the implication for the elicitation of anaphylaxis remains to be elucidated.

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author
; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
2S Albumins, Plant, Allergens, Antigens, Plant, Arachis, Humans, Peanut Hypersensitivity, Plant Proteins
in
Allergy
volume
75
issue
7
pages
9 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:85076147331
  • pmid:31715004
ISSN
1398-9995
DOI
10.1111/all.14109
language
English
LU publication?
no
additional info
© 2019 EAACI and John Wiley and Sons A/S. Published by John Wiley and Sons Ltd.
id
51839186-bdfb-413e-9e52-0fa4ce85b8b7
date added to LUP
2023-09-22 12:38:35
date last changed
2024-03-22 00:36:26
@article{51839186-bdfb-413e-9e52-0fa4ce85b8b7,
  abstract     = {{<p>BACKGROUND: There is mounting evidence that systemic uptake of food allergens is key to triggering anaphylaxis. However, direct proof for this theory is still lacking. The purpose of this study was to quantify the absorption and to determine the absorption kinetics of immunoreactive peanut protein in relation to the allergic response in human.</p><p>METHODS: Quantitative protein assays including mass spectrometry, dot blots and Western blotting were developed to determine the level of Ara h 2 absorption in human serum. The double monoclonal sandwich ELISA was applied to quantify absorbed Ara h 2 and 6, and the basophil histamine release assay and the human passive cutaneous anaphylaxis test were utilized to study the absorption kinetics of immunologically intact peanut proteins.</p><p>RESULTS: The protein assays worked but were not sensitive enough to trace the minute amounts of absorbed Ara h 2 in human serum. The level of Ara h 6 in serum was found to be up to 0.2 ng/mL, but Ara h 2 could not be detected with the ELISA. Both the in vivo and the in vitro methods were successful in demonstrating that: immunoreactive peanut protein was absorbed shortly after ingestion (≤5 minutes); the peanut protein concentration peaks between 1 and 4 hours; and peanut proteins can circulate for at least 48 hours in the bloodstream.</p><p>CONCLUSION: Ingested peanut protein is absorbed systemically and retains its immunoreactive capacity in human serum. However, the precise quantities and the implication for the elicitation of anaphylaxis remains to be elucidated.</p>}},
  author       = {{Pahlow Mose, Anja and Mortz, Ejvind and Stahl Skov, Per and Mortz, Charlotte Gotthard and Eller, Esben and Sprogøe, Ulrik and Barington, Torben and Bindslev-Jensen, Carsten}},
  issn         = {{1398-9995}},
  keywords     = {{2S Albumins, Plant; Allergens; Antigens, Plant; Arachis; Humans; Peanut Hypersensitivity; Plant Proteins}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{1721--1729}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Allergy}},
  title        = {{The quest for ingested peanut protein in human serum}},
  url          = {{http://dx.doi.org/10.1111/all.14109}},
  doi          = {{10.1111/all.14109}},
  volume       = {{75}},
  year         = {{2020}},
}