Changes in optical properties of human whole blood in vitro due to slow heating
(1997) In Photochemistry and Photobiology 65(2). p.366-373- Abstract
- Optical properties of human whole blood were investigated in vitro at 633 nm using a double integrating sphere set-up, The blood flow was maintained at a constant rate through a flow cell while continuously heating the blood at 0.2-1.1 degrees C/min from approximately 25 to 55 degrees C in a heat exchanger, A small, but rather abrupt decrease in the scattering asymmetry factor (g-factor) of 1.7 +/- 0.6% and a similar increase in the scattering coefficient of 2.9 +/- 0.6% were observed at approximately 45-46 degrees C yielding an increase in the reduced scattering coefficient of 40 +/- 10%. Furthermore, a continuous, manifest increase in the absorption coefficient was seen with increasing temperature, on average 80 +/- 70% from 25 to 50... (More)
- Optical properties of human whole blood were investigated in vitro at 633 nm using a double integrating sphere set-up, The blood flow was maintained at a constant rate through a flow cell while continuously heating the blood at 0.2-1.1 degrees C/min from approximately 25 to 55 degrees C in a heat exchanger, A small, but rather abrupt decrease in the scattering asymmetry factor (g-factor) of 1.7 +/- 0.6% and a similar increase in the scattering coefficient of 2.9 +/- 0.6% were observed at approximately 45-46 degrees C yielding an increase in the reduced scattering coefficient of 40 +/- 10%. Furthermore, a continuous, manifest increase in the absorption coefficient was seen with increasing temperature, on average 80 +/- 70% from 25 to 50 degrees C. The effect of the heating on the blood cells was also studied under a white-light transmission microscope, A sudden change in the shape of the red blood cells, from disc-shaped to spherical, was observed at approximately the same temperature at which the distinct changes in g-factor and scattering coefficient were observed, i.e. at 45-46 degrees C, The results indicate that this shape transformation could explain the sudden change in scattering properties. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/35057
- author
- Nilsson, A. M. K. ; Lucassen, G. W. ; Verkruysse, W. and van Gemert, M. J. C.
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Photochemistry and Photobiology
- volume
- 65
- issue
- 2
- pages
- 366 - 373
- publisher
- American Society for Photobiology
- external identifiers
-
- scopus:0031080405
- ISSN
- 0031-8655
- language
- English
- LU publication?
- no
- id
- 51d835f7-1857-4409-aec1-238d173d6986 (old id 35057)
- date added to LUP
- 2016-04-01 12:01:35
- date last changed
- 2022-04-13 04:59:29
@article{51d835f7-1857-4409-aec1-238d173d6986,
abstract = {{Optical properties of human whole blood were investigated in vitro at 633 nm using a double integrating sphere set-up, The blood flow was maintained at a constant rate through a flow cell while continuously heating the blood at 0.2-1.1 degrees C/min from approximately 25 to 55 degrees C in a heat exchanger, A small, but rather abrupt decrease in the scattering asymmetry factor (g-factor) of 1.7 +/- 0.6% and a similar increase in the scattering coefficient of 2.9 +/- 0.6% were observed at approximately 45-46 degrees C yielding an increase in the reduced scattering coefficient of 40 +/- 10%. Furthermore, a continuous, manifest increase in the absorption coefficient was seen with increasing temperature, on average 80 +/- 70% from 25 to 50 degrees C. The effect of the heating on the blood cells was also studied under a white-light transmission microscope, A sudden change in the shape of the red blood cells, from disc-shaped to spherical, was observed at approximately the same temperature at which the distinct changes in g-factor and scattering coefficient were observed, i.e. at 45-46 degrees C, The results indicate that this shape transformation could explain the sudden change in scattering properties.}},
author = {{Nilsson, A. M. K. and Lucassen, G. W. and Verkruysse, W. and van Gemert, M. J. C.}},
issn = {{0031-8655}},
language = {{eng}},
number = {{2}},
pages = {{366--373}},
publisher = {{American Society for Photobiology}},
series = {{Photochemistry and Photobiology}},
title = {{Changes in optical properties of human whole blood in vitro due to slow heating}},
url = {{https://lup.lub.lu.se/search/files/2748955/2370425.pdf}},
volume = {{65}},
year = {{1997}},
}