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Increased expression of ANAC017 primes for accelerated senescence

Broda, Martyna ; Khan, Kasim LU ; O'Leary, Brendan ; Pruzinska, Adriana ; Pong Lee, Chun ; Harvey Millar, A. and Van Aken, Olivier LU (2021) In Plant Physiology 186(4). p.2205-2221
Abstract

Recent studies in Arabidopsis (Arabidopsis thaliana) have reported conflicting roles for NAC DOMAIN CONTAINING PROTEIN 17 (ANAC017), a transcription factor regulating mitochondria-to-nuclear signaling, and its closest paralog NAC DOMAIN CONTAINING PROTEIN 16 (ANAC016), in leaf senescence. By synchronizing senescence in individually darkened leaves of knockout and overexpressing mutants from these contrasting studies, we demonstrate that elevated ANAC017 expression consistently causes accelerated senescence and cell death. A time-resolved transcriptome analysis revealed that senescence-associated pathways such as autophagy are not constitutively activated in ANAC017 overexpression lines, but require a senescence-stimulus to trigger... (More)

Recent studies in Arabidopsis (Arabidopsis thaliana) have reported conflicting roles for NAC DOMAIN CONTAINING PROTEIN 17 (ANAC017), a transcription factor regulating mitochondria-to-nuclear signaling, and its closest paralog NAC DOMAIN CONTAINING PROTEIN 16 (ANAC016), in leaf senescence. By synchronizing senescence in individually darkened leaves of knockout and overexpressing mutants from these contrasting studies, we demonstrate that elevated ANAC017 expression consistently causes accelerated senescence and cell death. A time-resolved transcriptome analysis revealed that senescence-associated pathways such as autophagy are not constitutively activated in ANAC017 overexpression lines, but require a senescence-stimulus to trigger accelerated induction. ANAC017 transcript and ANAC017-target genes are constitutively upregulated in ANAC017 overexpression lines, but surprisingly show a transient "super-induction" 1 d after senescence induction. This induction of ANAC017 and its target genes is observed during the later stages of age-related and dark-induced senescence, indicating the ANAC017 pathway is also activated in natural senescence. In contrast, knockout mutants of ANAC017 showed lowered senescence-induced induction of ANAC017 target genes during the late stages of dark-induced senescence. Finally, promoter binding analyses show that the ANAC016 promoter sequence is directly bound by ANAC017, so ANAC016 likely acts downstream of ANAC017 and is directly transcriptionally controlled by ANAC017 in a feed-forward loop during late senescence.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Plant Physiology
volume
186
issue
4
pages
17 pages
publisher
American Society of Plant Biologists
external identifiers
  • pmid:33914871
  • scopus:85112212167
ISSN
0032-0889
DOI
10.1093/plphys/kiab195
language
English
LU publication?
yes
id
51e7b620-e85b-4826-b5e4-dbf3da926857
date added to LUP
2021-09-23 17:00:23
date last changed
2024-06-15 16:44:06
@article{51e7b620-e85b-4826-b5e4-dbf3da926857,
  abstract     = {{<p>Recent studies in Arabidopsis (Arabidopsis thaliana) have reported conflicting roles for NAC DOMAIN CONTAINING PROTEIN 17 (ANAC017), a transcription factor regulating mitochondria-to-nuclear signaling, and its closest paralog NAC DOMAIN CONTAINING PROTEIN 16 (ANAC016), in leaf senescence. By synchronizing senescence in individually darkened leaves of knockout and overexpressing mutants from these contrasting studies, we demonstrate that elevated ANAC017 expression consistently causes accelerated senescence and cell death. A time-resolved transcriptome analysis revealed that senescence-associated pathways such as autophagy are not constitutively activated in ANAC017 overexpression lines, but require a senescence-stimulus to trigger accelerated induction. ANAC017 transcript and ANAC017-target genes are constitutively upregulated in ANAC017 overexpression lines, but surprisingly show a transient "super-induction" 1 d after senescence induction. This induction of ANAC017 and its target genes is observed during the later stages of age-related and dark-induced senescence, indicating the ANAC017 pathway is also activated in natural senescence. In contrast, knockout mutants of ANAC017 showed lowered senescence-induced induction of ANAC017 target genes during the late stages of dark-induced senescence. Finally, promoter binding analyses show that the ANAC016 promoter sequence is directly bound by ANAC017, so ANAC016 likely acts downstream of ANAC017 and is directly transcriptionally controlled by ANAC017 in a feed-forward loop during late senescence.</p>}},
  author       = {{Broda, Martyna and Khan, Kasim and O'Leary, Brendan and Pruzinska, Adriana and Pong Lee, Chun and Harvey Millar, A. and Van Aken, Olivier}},
  issn         = {{0032-0889}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{2205--2221}},
  publisher    = {{American Society of Plant Biologists}},
  series       = {{Plant Physiology}},
  title        = {{Increased expression of ANAC017 primes for accelerated senescence}},
  url          = {{http://dx.doi.org/10.1093/plphys/kiab195}},
  doi          = {{10.1093/plphys/kiab195}},
  volume       = {{186}},
  year         = {{2021}},
}