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Protocol for Proteome Analysis of Group A Streptococcus

Wilk, Laura LU (2020) In Methods in Molecular Biology 2136. p.135-144
Abstract

This chapter describes a protocol for the separation of different cell fractions from Streptococcus pyogenes and sample preparation for analysis by mass spectrometry. The presented approach can be used for the analysis of all subcellular proteomes from S. pyogenes and enables identification of drug or vaccine targets within a certain cellular fraction. Here, proteins integral to the plasma membrane are of particular interest for the development of new antimicrobial therapies.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Bacterial proteome, Cell wall, Cellular fractionation, Gram-positive bacteria, Mass spectrometry, Membrane, secretome, Streptococcus pyogenes
host publication
Group A Streptococcus : Methods and Protocols - Methods and Protocols
series title
Methods in Molecular Biology
editor
Proft, Thomas and Loh, Jacelyn M.S.
volume
2136
pages
10 pages
publisher
Humana Press
external identifiers
  • pmid:32430817
  • scopus:85085155994
ISSN
1064-3745
1940-6029
ISBN
978-1-0716-0467-0
978-1-0716-0466-3
DOI
10.1007/978-1-0716-0467-0_9
language
English
LU publication?
yes
id
522da726-eb8a-423d-8e22-e1f68d5dc78c
date added to LUP
2020-06-26 12:01:01
date last changed
2020-06-27 01:58:41
@inbook{522da726-eb8a-423d-8e22-e1f68d5dc78c,
  abstract     = {<p>This chapter describes a protocol for the separation of different cell fractions from Streptococcus pyogenes and sample preparation for analysis by mass spectrometry. The presented approach can be used for the analysis of all subcellular proteomes from S. pyogenes and enables identification of drug or vaccine targets within a certain cellular fraction. Here, proteins integral to the plasma membrane are of particular interest for the development of new antimicrobial therapies.</p>},
  author       = {Wilk, Laura},
  booktitle    = {Group A Streptococcus : Methods and Protocols},
  editor       = {Proft, Thomas and Loh, Jacelyn M.S.},
  isbn         = {978-1-0716-0467-0},
  issn         = {1064-3745},
  language     = {eng},
  month        = {05},
  pages        = {135--144},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Protocol for Proteome Analysis of Group A Streptococcus},
  url          = {http://dx.doi.org/10.1007/978-1-0716-0467-0_9},
  doi          = {10.1007/978-1-0716-0467-0_9},
  volume       = {2136},
  year         = {2020},
}