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Release of cytokines and proteases from human peripheral blood mononuclear and polymorphonuclear cells following phagocytosis and LPS stimulation

Ohlsson, K.; Linder, C.; Lundberg, E. and Axelsson, L. LU (1996) In Scandinavian Journal of Clinical and Laboratory Investigation 56(5). p.461-470
Abstract

Release and cellular contents of pro- and anti-inflammatory cytokines, neutrophilic elastase and secretory leukocyte proteinase inhibitor (SLPI) were measured with enzyme-linked immunosorbent assay in peripheral blood mono- and polymorphonuclear cells stimulated with preopsonized yeast cells or lipopolysaccharide. Tumour necrosis factor alpha (TNFα) was also measured with a bioassay. TNFα production and soluble TNFα receptor I (sTNF RI) were demonstrated in the environment of both cell populations. The bioassay indicated levels of TNFα far below those detected by ELISA. The overall secretion of cytokines and their inhibitors was found to favour an anti-inflammatory balance in the environment of the stimulated cells. The interleukin-1... (More)

Release and cellular contents of pro- and anti-inflammatory cytokines, neutrophilic elastase and secretory leukocyte proteinase inhibitor (SLPI) were measured with enzyme-linked immunosorbent assay in peripheral blood mono- and polymorphonuclear cells stimulated with preopsonized yeast cells or lipopolysaccharide. Tumour necrosis factor alpha (TNFα) was also measured with a bioassay. TNFα production and soluble TNFα receptor I (sTNF RI) were demonstrated in the environment of both cell populations. The bioassay indicated levels of TNFα far below those detected by ELISA. The overall secretion of cytokines and their inhibitors was found to favour an anti-inflammatory balance in the environment of the stimulated cells. The interleukin-1 receptor antagonist (IL1-ra), compared with interleukin-1β (IL-1β), dominated the secretions from both cell types with a 100- to 1000-fold excess respectively. Most of the translated IL-1β was not secreted but found associated with the cellular compartments. In contrast to lipopolysaccharide (LPS) stimulation, preopsonized yeast cells stimulated a massive release of elastase from neutrophil cells.

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organization
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Contribution to journal
publication status
published
subject
keywords
Cytokines, Interleukin-1, Interleukin-1 receptor antagonist, Leukocyte proteases, Secretory leukocyte proteinase inhibitor, Soluble tumour necrosis factor receptor I, Tumour necrosis factor
in
Scandinavian Journal of Clinical and Laboratory Investigation
volume
56
issue
5
pages
10 pages
publisher
Informa Healthcare
external identifiers
  • scopus:0029816325
ISSN
0036-5513
language
English
LU publication?
yes
id
524a69e7-8302-4aa8-b6c8-d79ae97d3df8
date added to LUP
2017-03-10 13:16:15
date last changed
2017-03-10 13:16:15
@article{524a69e7-8302-4aa8-b6c8-d79ae97d3df8,
  abstract     = {<p>Release and cellular contents of pro- and anti-inflammatory cytokines, neutrophilic elastase and secretory leukocyte proteinase inhibitor (SLPI) were measured with enzyme-linked immunosorbent assay in peripheral blood mono- and polymorphonuclear cells stimulated with preopsonized yeast cells or lipopolysaccharide. Tumour necrosis factor alpha (TNFα) was also measured with a bioassay. TNFα production and soluble TNFα receptor I (sTNF RI) were demonstrated in the environment of both cell populations. The bioassay indicated levels of TNFα far below those detected by ELISA. The overall secretion of cytokines and their inhibitors was found to favour an anti-inflammatory balance in the environment of the stimulated cells. The interleukin-1 receptor antagonist (IL1-ra), compared with interleukin-1β (IL-1β), dominated the secretions from both cell types with a 100- to 1000-fold excess respectively. Most of the translated IL-1β was not secreted but found associated with the cellular compartments. In contrast to lipopolysaccharide (LPS) stimulation, preopsonized yeast cells stimulated a massive release of elastase from neutrophil cells.</p>},
  author       = {Ohlsson, K. and Linder, C. and Lundberg, E. and Axelsson, L.},
  issn         = {0036-5513},
  keyword      = {Cytokines,Interleukin-1,Interleukin-1 receptor antagonist,Leukocyte proteases,Secretory leukocyte proteinase inhibitor,Soluble tumour necrosis factor receptor I,Tumour necrosis factor},
  language     = {eng},
  number       = {5},
  pages        = {461--470},
  publisher    = {Informa Healthcare},
  series       = {Scandinavian Journal of Clinical and Laboratory Investigation},
  title        = {Release of cytokines and proteases from human peripheral blood mononuclear and polymorphonuclear cells following phagocytosis and LPS stimulation},
  volume       = {56},
  year         = {1996},
}