Profiling of small RNA cargo of extracellular vesicles shed by Trypanosoma cruzi reveals a specific extracellular signature.
Fernandez-Calero, Tamara ; Garcia-Silva, Rosa ; Pena, Alvaro ; Robello, Carlos ; Persson, Helena LU
; Rovira, Carlos
LU
; Naya, Hugo
LU
and Cayota, Alfonso
(2015)
In Molecular and Biochemical Parasitology
199(1-2).
p.19-28
- Abstract
- Over the last years, an expanding family of small regulatory RNAs (e.g. microRNAs, siRNAs and piRNAs) was recognized as key players in novel forms of post-transcriptional gene regulation in most eukaryotes. However, the machinery associated with Ago/Dicer-dependent small RNA biogenesis was thought to be either entirely lost or extensively simplified in some unicellular organisms including Trypanosoma cruzi, Saccharomyces cerevisiae, Leishmania major and Plasmodium falciparum. Although the biogenesis of small RNAs from non-coding RNAs represent a minor fraction of the normal small RNA transcriptome in eukaryotic cells, they represent the unique small RNA pathways in Trypanosoma cruzi which produce different populations of small RNAs derived... (More)
- Over the last years, an expanding family of small regulatory RNAs (e.g. microRNAs, siRNAs and piRNAs) was recognized as key players in novel forms of post-transcriptional gene regulation in most eukaryotes. However, the machinery associated with Ago/Dicer-dependent small RNA biogenesis was thought to be either entirely lost or extensively simplified in some unicellular organisms including Trypanosoma cruzi, Saccharomyces cerevisiae, Leishmania major and Plasmodium falciparum. Although the biogenesis of small RNAs from non-coding RNAs represent a minor fraction of the normal small RNA transcriptome in eukaryotic cells, they represent the unique small RNA pathways in Trypanosoma cruzi which produce different populations of small RNAs derived from tRNAs, rRNAs, sn/snoRNAs and mRNAs. These small RNAs are secreted included in extracellular vesicles and transferred to other parasites and susceptible mammalian cells. This process represents a novel form of cross-kingdom transfer of genetic material suggesting that secreted vesicles could represent new relevant pieces in life cycle transitions, infectivity and cell-to-cell communication. Here, we provide for the first time a detailed analysis of the small RNA cargo of extracellular vesicles from T. cruzi epimastigotes under nutritional stress conditions compared to the respective intracellular compartment using deep sequencing. Compared with the intracellular compartment, shed extracellular vesicles showed a specific extracellular signature conformed by distinctive patterns of small RNAs derived from rRNA, tRNA, sno/snRNAs and protein coding sequences which evidenced specific secretory small RNA processing pathways. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5257965
- author
- Fernandez-Calero, Tamara
; Garcia-Silva, Rosa
; Pena, Alvaro
; Robello, Carlos
; Persson, Helena
LU
; Rovira, Carlos
LU
; Naya, Hugo
LU
and Cayota, Alfonso
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular and Biochemical Parasitology
- volume
- 199
- issue
- 1-2
- pages
- 19 - 28
- publisher
- Elsevier
- external identifiers
-
- pmid:25795082
- wos:000355362300004
- scopus:84964282384
- pmid:25795082
- ISSN
- 1872-9428
- DOI
- 10.1016/j.molbiopara.2015.03.003
- language
- English
- LU publication?
- yes
- id
- 01865069-c682-4480-ae83-2e42fb687ba8 (old id 5257965)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/25795082?dopt=Abstract
- date added to LUP
- 2016-04-01 09:49:07
- date last changed
- 2022-04-11 23:11:38
@article{01865069-c682-4480-ae83-2e42fb687ba8,
abstract = {{Over the last years, an expanding family of small regulatory RNAs (e.g. microRNAs, siRNAs and piRNAs) was recognized as key players in novel forms of post-transcriptional gene regulation in most eukaryotes. However, the machinery associated with Ago/Dicer-dependent small RNA biogenesis was thought to be either entirely lost or extensively simplified in some unicellular organisms including Trypanosoma cruzi, Saccharomyces cerevisiae, Leishmania major and Plasmodium falciparum. Although the biogenesis of small RNAs from non-coding RNAs represent a minor fraction of the normal small RNA transcriptome in eukaryotic cells, they represent the unique small RNA pathways in Trypanosoma cruzi which produce different populations of small RNAs derived from tRNAs, rRNAs, sn/snoRNAs and mRNAs. These small RNAs are secreted included in extracellular vesicles and transferred to other parasites and susceptible mammalian cells. This process represents a novel form of cross-kingdom transfer of genetic material suggesting that secreted vesicles could represent new relevant pieces in life cycle transitions, infectivity and cell-to-cell communication. Here, we provide for the first time a detailed analysis of the small RNA cargo of extracellular vesicles from T. cruzi epimastigotes under nutritional stress conditions compared to the respective intracellular compartment using deep sequencing. Compared with the intracellular compartment, shed extracellular vesicles showed a specific extracellular signature conformed by distinctive patterns of small RNAs derived from rRNA, tRNA, sno/snRNAs and protein coding sequences which evidenced specific secretory small RNA processing pathways.}},
author = {{Fernandez-Calero, Tamara and Garcia-Silva, Rosa and Pena, Alvaro and Robello, Carlos and Persson, Helena and Rovira, Carlos and Naya, Hugo and Cayota, Alfonso}},
issn = {{1872-9428}},
language = {{eng}},
number = {{1-2}},
pages = {{19--28}},
publisher = {{Elsevier}},
series = {{Molecular and Biochemical Parasitology}},
title = {{Profiling of small RNA cargo of extracellular vesicles shed by Trypanosoma cruzi reveals a specific extracellular signature.}},
url = {{http://dx.doi.org/10.1016/j.molbiopara.2015.03.003}},
doi = {{10.1016/j.molbiopara.2015.03.003}},
volume = {{199}},
year = {{2015}},
}