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Profiling of small RNA cargo of extracellular vesicles shed by Trypanosoma cruzi reveals a specific extracellular signature.

Fernandez-Calero, Tamara; Garcia-Silva, Rosa; Pena, Alvaro; Robello, Carlos; Persson, Helena LU ; Rovira, Carlos LU ; Naya, Hugo LU and Cayota, Alfonso (2015) In Molecular and Biochemical Parasitology 199(1-2). p.19-28
Abstract
Over the last years, an expanding family of small regulatory RNAs (e.g. microRNAs, siRNAs and piRNAs) was recognized as key players in novel forms of post-transcriptional gene regulation in most eukaryotes. However, the machinery associated with Ago/Dicer-dependent small RNA biogenesis was thought to be either entirely lost or extensively simplified in some unicellular organisms including Trypanosoma cruzi, Saccharomyces cerevisiae, Leishmania major and Plasmodium falciparum. Although the biogenesis of small RNAs from non-coding RNAs represent a minor fraction of the normal small RNA transcriptome in eukaryotic cells, they represent the unique small RNA pathways in Trypanosoma cruzi which produce different populations of small RNAs derived... (More)
Over the last years, an expanding family of small regulatory RNAs (e.g. microRNAs, siRNAs and piRNAs) was recognized as key players in novel forms of post-transcriptional gene regulation in most eukaryotes. However, the machinery associated with Ago/Dicer-dependent small RNA biogenesis was thought to be either entirely lost or extensively simplified in some unicellular organisms including Trypanosoma cruzi, Saccharomyces cerevisiae, Leishmania major and Plasmodium falciparum. Although the biogenesis of small RNAs from non-coding RNAs represent a minor fraction of the normal small RNA transcriptome in eukaryotic cells, they represent the unique small RNA pathways in Trypanosoma cruzi which produce different populations of small RNAs derived from tRNAs, rRNAs, sn/snoRNAs and mRNAs. These small RNAs are secreted included in extracellular vesicles and transferred to other parasites and susceptible mammalian cells. This process represents a novel form of cross-kingdom transfer of genetic material suggesting that secreted vesicles could represent new relevant pieces in life cycle transitions, infectivity and cell-to-cell communication. Here, we provide for the first time a detailed analysis of the small RNA cargo of extracellular vesicles from T. cruzi epimastigotes under nutritional stress conditions compared to the respective intracellular compartment using deep sequencing. Compared with the intracellular compartment, shed extracellular vesicles showed a specific extracellular signature conformed by distinctive patterns of small RNAs derived from rRNA, tRNA, sno/snRNAs and protein coding sequences which evidenced specific secretory small RNA processing pathways. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular and Biochemical Parasitology
volume
199
issue
1-2
pages
19 - 28
publisher
Elsevier
external identifiers
  • pmid:25795082
  • wos:000355362300004
  • scopus:84964282384
ISSN
1872-9428
DOI
10.1016/j.molbiopara.2015.03.003
language
English
LU publication?
yes
id
01865069-c682-4480-ae83-2e42fb687ba8 (old id 5257965)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25795082?dopt=Abstract
date added to LUP
2015-04-05 14:07:22
date last changed
2017-06-25 03:00:33
@article{01865069-c682-4480-ae83-2e42fb687ba8,
  abstract     = {Over the last years, an expanding family of small regulatory RNAs (e.g. microRNAs, siRNAs and piRNAs) was recognized as key players in novel forms of post-transcriptional gene regulation in most eukaryotes. However, the machinery associated with Ago/Dicer-dependent small RNA biogenesis was thought to be either entirely lost or extensively simplified in some unicellular organisms including Trypanosoma cruzi, Saccharomyces cerevisiae, Leishmania major and Plasmodium falciparum. Although the biogenesis of small RNAs from non-coding RNAs represent a minor fraction of the normal small RNA transcriptome in eukaryotic cells, they represent the unique small RNA pathways in Trypanosoma cruzi which produce different populations of small RNAs derived from tRNAs, rRNAs, sn/snoRNAs and mRNAs. These small RNAs are secreted included in extracellular vesicles and transferred to other parasites and susceptible mammalian cells. This process represents a novel form of cross-kingdom transfer of genetic material suggesting that secreted vesicles could represent new relevant pieces in life cycle transitions, infectivity and cell-to-cell communication. Here, we provide for the first time a detailed analysis of the small RNA cargo of extracellular vesicles from T. cruzi epimastigotes under nutritional stress conditions compared to the respective intracellular compartment using deep sequencing. Compared with the intracellular compartment, shed extracellular vesicles showed a specific extracellular signature conformed by distinctive patterns of small RNAs derived from rRNA, tRNA, sno/snRNAs and protein coding sequences which evidenced specific secretory small RNA processing pathways.},
  author       = {Fernandez-Calero, Tamara and Garcia-Silva, Rosa and Pena, Alvaro and Robello, Carlos and Persson, Helena and Rovira, Carlos and Naya, Hugo and Cayota, Alfonso},
  issn         = {1872-9428},
  language     = {eng},
  number       = {1-2},
  pages        = {19--28},
  publisher    = {Elsevier},
  series       = {Molecular and Biochemical Parasitology},
  title        = {Profiling of small RNA cargo of extracellular vesicles shed by Trypanosoma cruzi reveals a specific extracellular signature.},
  url          = {http://dx.doi.org/10.1016/j.molbiopara.2015.03.003},
  volume       = {199},
  year         = {2015},
}