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On-Demand Delivery of Single DNA Molecules Using Nanopipets.

Ivanov, Aleksandar P; Actis, Paolo; Jönsson, Peter LU ; Klenerman, David; Korchev, Yuri and Edel, Joshua B (2015) In ACS Nano 9(4). p.3587-3595
Abstract
Understanding the behavioral properties of single molecules or larger scale populations interacting with single molecules is currently a hotly pursued topic in nanotechnology. This arises from the potential such techniques have in relation to applications such as targeted drug delivery, early stage detection of disease, and drug screening. Although label and label-free single molecule detection strategies have existed for a number of years, currently lacking are efficient methods for the controllable delivery of single molecules in aqueous environments. In this article we show both experimentally and from simulations that nanopipets in conjunction with asymmetric voltage pulses can be used for label-free detection and delivery of single... (More)
Understanding the behavioral properties of single molecules or larger scale populations interacting with single molecules is currently a hotly pursued topic in nanotechnology. This arises from the potential such techniques have in relation to applications such as targeted drug delivery, early stage detection of disease, and drug screening. Although label and label-free single molecule detection strategies have existed for a number of years, currently lacking are efficient methods for the controllable delivery of single molecules in aqueous environments. In this article we show both experimentally and from simulations that nanopipets in conjunction with asymmetric voltage pulses can be used for label-free detection and delivery of single molecules through the tip of a nanopipet with "on-demand" timing resolution. This was demonstrated by controllable delivery of 5 kbp and 10 kbp DNA molecules from solutions with concentrations as low as 3 pM. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
ACS Nano
volume
9
issue
4
pages
3587 - 3595
publisher
The American Chemical Society
external identifiers
  • pmid:25794527
  • wos:000353867000021
  • scopus:84928950441
ISSN
1936-086X
DOI
10.1021/acsnano.5b00911
language
English
LU publication?
yes
id
b03cef99-9f63-44ab-93af-f3a6ab239f2b (old id 5257976)
date added to LUP
2015-04-07 19:30:04
date last changed
2017-11-19 03:03:04
@article{b03cef99-9f63-44ab-93af-f3a6ab239f2b,
  abstract     = {Understanding the behavioral properties of single molecules or larger scale populations interacting with single molecules is currently a hotly pursued topic in nanotechnology. This arises from the potential such techniques have in relation to applications such as targeted drug delivery, early stage detection of disease, and drug screening. Although label and label-free single molecule detection strategies have existed for a number of years, currently lacking are efficient methods for the controllable delivery of single molecules in aqueous environments. In this article we show both experimentally and from simulations that nanopipets in conjunction with asymmetric voltage pulses can be used for label-free detection and delivery of single molecules through the tip of a nanopipet with "on-demand" timing resolution. This was demonstrated by controllable delivery of 5 kbp and 10 kbp DNA molecules from solutions with concentrations as low as 3 pM.},
  author       = {Ivanov, Aleksandar P and Actis, Paolo and Jönsson, Peter and Klenerman, David and Korchev, Yuri and Edel, Joshua B},
  issn         = {1936-086X},
  language     = {eng},
  number       = {4},
  pages        = {3587--3595},
  publisher    = {The American Chemical Society},
  series       = {ACS Nano},
  title        = {On-Demand Delivery of Single DNA Molecules Using Nanopipets.},
  url          = {http://dx.doi.org/10.1021/acsnano.5b00911},
  volume       = {9},
  year         = {2015},
}