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The carbon starvation response of the ectomycorrhizal fungus Paxillus involutus.

Ellström, Magnus LU ; Shah, Firoz LU ; Johansson, Tomas LU ; Ahrén, Dag LU ; Persson, Per LU and Tunlid, Anders LU (2015) In FEMS Microbiology Ecology 91(4).
Abstract
The amounts of carbon allocated to the fungal partner in ectomycorrhizal associations can vary substantially depending on the plant growth and the soil nutrient conditions, and the fungus may frequently be confronted with limitations in carbon. We used chemical analysis and transcriptome profiling to examine the physiological response of the ectomycorrhizal fungus Paxillus involutus to carbon starvation during axenic cultivation. Carbon starvation induced a decrease in the biomass. Concomitantly, ammonium, cell-wall material (chitin) and proteolytic enzymes were released into the medium, which suggest autolysis. Compared with the transcriptome of actively growing hyphae, about 45% of the transcripts analyzed were differentially regulated... (More)
The amounts of carbon allocated to the fungal partner in ectomycorrhizal associations can vary substantially depending on the plant growth and the soil nutrient conditions, and the fungus may frequently be confronted with limitations in carbon. We used chemical analysis and transcriptome profiling to examine the physiological response of the ectomycorrhizal fungus Paxillus involutus to carbon starvation during axenic cultivation. Carbon starvation induced a decrease in the biomass. Concomitantly, ammonium, cell-wall material (chitin) and proteolytic enzymes were released into the medium, which suggest autolysis. Compared with the transcriptome of actively growing hyphae, about 45% of the transcripts analyzed were differentially regulated during C-starvation. Induced during starvation were transcripts encoding extracellular enzymes such as peptidases, chitinases, and laccases. In parallel, transcripts of N-transporters were upregulated, which suggest that some of the released nitrogen compounds were re-assimilated by the mycelium. The observed changes suggest that the carbon-starvation response in P. involutus is associated with complex cellular changes that involves autolysis, recycling of intracellular compounds by autophagy and reabsorption of the extracellular released material. The study provides molecular markers that can be used to examine the role of autolysis for the turnover and survival of the ectomycorrhizal mycelium in soils. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
FEMS Microbiology Ecology
volume
91
issue
4
publisher
Elsevier
external identifiers
  • pmid:25778509
  • wos:000355327300010
  • scopus:84954239015
ISSN
1574-6941
DOI
10.1093/femsec/fiv027
project
MICCS - Molecular Interactions Controlling soil Carbon Sequestration
language
English
LU publication?
yes
id
ab2fff3e-c53d-47cd-9f90-c9ef3445cf2c (old id 5258455)
date added to LUP
2015-04-07 19:20:28
date last changed
2017-08-13 03:15:14
@article{ab2fff3e-c53d-47cd-9f90-c9ef3445cf2c,
  abstract     = {The amounts of carbon allocated to the fungal partner in ectomycorrhizal associations can vary substantially depending on the plant growth and the soil nutrient conditions, and the fungus may frequently be confronted with limitations in carbon. We used chemical analysis and transcriptome profiling to examine the physiological response of the ectomycorrhizal fungus Paxillus involutus to carbon starvation during axenic cultivation. Carbon starvation induced a decrease in the biomass. Concomitantly, ammonium, cell-wall material (chitin) and proteolytic enzymes were released into the medium, which suggest autolysis. Compared with the transcriptome of actively growing hyphae, about 45% of the transcripts analyzed were differentially regulated during C-starvation. Induced during starvation were transcripts encoding extracellular enzymes such as peptidases, chitinases, and laccases. In parallel, transcripts of N-transporters were upregulated, which suggest that some of the released nitrogen compounds were re-assimilated by the mycelium. The observed changes suggest that the carbon-starvation response in P. involutus is associated with complex cellular changes that involves autolysis, recycling of intracellular compounds by autophagy and reabsorption of the extracellular released material. The study provides molecular markers that can be used to examine the role of autolysis for the turnover and survival of the ectomycorrhizal mycelium in soils.},
  author       = {Ellström, Magnus and Shah, Firoz and Johansson, Tomas and Ahrén, Dag and Persson, Per and Tunlid, Anders},
  issn         = {1574-6941},
  language     = {eng},
  number       = {4},
  publisher    = {Elsevier},
  series       = {FEMS Microbiology Ecology},
  title        = {The carbon starvation response of the ectomycorrhizal fungus Paxillus involutus.},
  url          = {http://dx.doi.org/10.1093/femsec/fiv027},
  volume       = {91},
  year         = {2015},
}