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Human adipose cells in vitro are either refractory or responsive to insulin, reflecting host metabolic state.

Lizunov, Vladimir A; Stenkula, Karin LU ; Blank, Paul S; Troy, Aaron; Lee, Jo-Ping; Skarulis, Monica C; Cushman, Samuel W and Zimmerberg, Joshua (2015) In PLoS ONE 10(3).
Abstract
While intercellular communication processes are frequently characterized by switch-like transitions, the endocrine system, including the adipose tissue response to insulin, has been characterized by graded responses. Yet here individual cells from adipose tissue biopsies are best described by a switch-like transition between the basal and insulin-stimulated states for the trafficking of the glucose transporter GLUT4. Two statistically-defined populations best describe the observed cellular heterogeneity, representing the fractions of refractive and responsive adipose cells. Furthermore, subjects exhibiting high systemic insulin sensitivity indices (SI) have high fractions of responsive adipose cells in vitro, while subjects exhibiting... (More)
While intercellular communication processes are frequently characterized by switch-like transitions, the endocrine system, including the adipose tissue response to insulin, has been characterized by graded responses. Yet here individual cells from adipose tissue biopsies are best described by a switch-like transition between the basal and insulin-stimulated states for the trafficking of the glucose transporter GLUT4. Two statistically-defined populations best describe the observed cellular heterogeneity, representing the fractions of refractive and responsive adipose cells. Furthermore, subjects exhibiting high systemic insulin sensitivity indices (SI) have high fractions of responsive adipose cells in vitro, while subjects exhibiting decreasing SI have increasing fractions of refractory cells in vitro. Thus, a two-component model best describes the relationship between cellular refractory fraction and subject SI. Since isolated cells exhibit these different response characteristics in the presence of constant culture conditions and milieu, we suggest that a physiological switching mechanism at the adipose cellular level ultimately drives systemic SI. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
PLoS ONE
volume
10
issue
3
publisher
Public Library of Science
external identifiers
  • pmid:25768970
  • wos:000351277500074
  • scopus:84929501022
ISSN
1932-6203
DOI
10.1371/journal.pone.0119291
language
English
LU publication?
yes
id
b4930a8b-d142-4275-9315-fb0e34df3f1a (old id 5258746)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25768970?dopt=Abstract
date added to LUP
2015-04-03 18:53:06
date last changed
2017-01-01 06:03:58
@article{b4930a8b-d142-4275-9315-fb0e34df3f1a,
  abstract     = {While intercellular communication processes are frequently characterized by switch-like transitions, the endocrine system, including the adipose tissue response to insulin, has been characterized by graded responses. Yet here individual cells from adipose tissue biopsies are best described by a switch-like transition between the basal and insulin-stimulated states for the trafficking of the glucose transporter GLUT4. Two statistically-defined populations best describe the observed cellular heterogeneity, representing the fractions of refractive and responsive adipose cells. Furthermore, subjects exhibiting high systemic insulin sensitivity indices (SI) have high fractions of responsive adipose cells in vitro, while subjects exhibiting decreasing SI have increasing fractions of refractory cells in vitro. Thus, a two-component model best describes the relationship between cellular refractory fraction and subject SI. Since isolated cells exhibit these different response characteristics in the presence of constant culture conditions and milieu, we suggest that a physiological switching mechanism at the adipose cellular level ultimately drives systemic SI.},
  articleno    = {e0119291},
  author       = {Lizunov, Vladimir A and Stenkula, Karin and Blank, Paul S and Troy, Aaron and Lee, Jo-Ping and Skarulis, Monica C and Cushman, Samuel W and Zimmerberg, Joshua},
  issn         = {1932-6203},
  language     = {eng},
  number       = {3},
  publisher    = {Public Library of Science},
  series       = {PLoS ONE},
  title        = {Human adipose cells in vitro are either refractory or responsive to insulin, reflecting host metabolic state.},
  url          = {http://dx.doi.org/10.1371/journal.pone.0119291},
  volume       = {10},
  year         = {2015},
}