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Radiolabeling and Biotinylation of Internalizing Monoclonal Antibody Chimeric BR96: Potential Use for Extracorporeal Immunoadsorption.

Chen, Jianqing LU (1997)
Abstract
In this thesis, methodology of radiolabeling and simultaneous biotinylation for internalizing monoclonal antibody (MAb) chimeric BR96 (chiBR96) have been investigated by using three element groups of potential therapeutic radionuclides iodine, indium and rhenium, and their different labeling methods. The biodistribution and kinetics of biotinylated and radiolabeled chiBR96 have been studied in colon carcinoma isografted rats. The potential use of ECIA, based on the biotin-avidin concept, has been evaluated and compared with the approach of avidin "chase" in the same animal tumor model with respect to an enhancement of tumor-to-normal tissue (T/N) activity ratio. In vivo stability and tumor targeting capacity of biotinylated 111In-chiBR96... (More)
In this thesis, methodology of radiolabeling and simultaneous biotinylation for internalizing monoclonal antibody (MAb) chimeric BR96 (chiBR96) have been investigated by using three element groups of potential therapeutic radionuclides iodine, indium and rhenium, and their different labeling methods. The biodistribution and kinetics of biotinylated and radiolabeled chiBR96 have been studied in colon carcinoma isografted rats. The potential use of ECIA, based on the biotin-avidin concept, has been evaluated and compared with the approach of avidin "chase" in the same animal tumor model with respect to an enhancement of tumor-to-normal tissue (T/N) activity ratio. In vivo stability and tumor targeting capacity of biotinylated 111In-chiBR96 using the chelate SCN-Bz-CHX-A-DTPA, have increased compared with two other chelates used in this study. Conjugate NSTBB has been successfully used for iodination of chiBR96 combined with biotinylation, in contrast to the combination with electrophilic labeling method (Less)
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author
supervisor
opponent
  • Fritzberg, Alan R., Professor, NeoRx Co., Seattle, WA, USA
organization
publishing date
type
Thesis
publication status
published
subject
keywords
serologi, Immunologi, transplantation, Immunology, serology
pages
122 pages
publisher
Radiation Physics, Lund
defense location
Conference room at Department of Oncology
defense date
1997-02-05 10:15:00
external identifiers
  • other:ISRN: LUMEDW/MERI--97/1001--SE
ISBN
91-628-2329-9
language
English
LU publication?
yes
id
5258a1dc-5ba4-4a9b-bbc6-5eadd891944e (old id 28937)
date added to LUP
2016-04-04 11:41:10
date last changed
2018-11-21 21:06:30
@phdthesis{5258a1dc-5ba4-4a9b-bbc6-5eadd891944e,
  abstract     = {{In this thesis, methodology of radiolabeling and simultaneous biotinylation for internalizing monoclonal antibody (MAb) chimeric BR96 (chiBR96) have been investigated by using three element groups of potential therapeutic radionuclides iodine, indium and rhenium, and their different labeling methods. The biodistribution and kinetics of biotinylated and radiolabeled chiBR96 have been studied in colon carcinoma isografted rats. The potential use of ECIA, based on the biotin-avidin concept, has been evaluated and compared with the approach of avidin "chase" in the same animal tumor model with respect to an enhancement of tumor-to-normal tissue (T/N) activity ratio. In vivo stability and tumor targeting capacity of biotinylated 111In-chiBR96 using the chelate SCN-Bz-CHX-A-DTPA, have increased compared with two other chelates used in this study. Conjugate NSTBB has been successfully used for iodination of chiBR96 combined with biotinylation, in contrast to the combination with electrophilic labeling method}},
  author       = {{Chen, Jianqing}},
  isbn         = {{91-628-2329-9}},
  keywords     = {{serologi; Immunologi; transplantation; Immunology; serology}},
  language     = {{eng}},
  publisher    = {{Radiation Physics, Lund}},
  school       = {{Lund University}},
  title        = {{Radiolabeling and Biotinylation of Internalizing Monoclonal Antibody Chimeric BR96: Potential Use for Extracorporeal Immunoadsorption.}},
  year         = {{1997}},
}