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Scaffolding the retina: The interstitial extracellular matrix during rat retinal development.

Taylor, Linnéa LU ; Arnér, Karin LU ; Engelsberg, Karl LU and Ghosh, Fredrik LU (2015) In International Journal of Developmental Neuroscience 42. p.46-58
Abstract
PURPOSE: To examine the expression of interstitial extracellular matrix components and their role during retinal development.

MATERIAL AND METHODS: Fibronectin (FN), collagen IV (Coll IV) and laminin 5 (Lam 5) expression in rat retinas from developmental stages E17 to adult were studied. In addition, PN5 full-thickness retinas were cultured for 7 days with dispase, which selectively cleaves FN and Coll IV, at either 0.5U/ml or 5.0U/ml for 3 or 24h. Eyecups and retinal cultures were examined morphologically using hematoxylin and eosin staining and immunohistochemistry.

RESULTS: Coll IV, Lam 5 and FN were all transiently expressed in the interstitial matrix of the retinal layers during development. The retinal layers in... (More)
PURPOSE: To examine the expression of interstitial extracellular matrix components and their role during retinal development.

MATERIAL AND METHODS: Fibronectin (FN), collagen IV (Coll IV) and laminin 5 (Lam 5) expression in rat retinas from developmental stages E17 to adult were studied. In addition, PN5 full-thickness retinas were cultured for 7 days with dispase, which selectively cleaves FN and Coll IV, at either 0.5U/ml or 5.0U/ml for 3 or 24h. Eyecups and retinal cultures were examined morphologically using hematoxylin and eosin staining and immunohistochemistry.

RESULTS: Coll IV, Lam 5 and FN were all transiently expressed in the interstitial matrix of the retinal layers during development. The retinal layers in dispase treated explants was severely disturbed in a dose and time dependent manner.

CONCLUSIONS: FN, Lam 5 and Coll IV, are present in the interstitial extracellular matrix during rat retinal development. Enzymatic cleavage of FN and Coll IV early in the lamination process disrupts the retinal layers implicating their pivotal role in this process. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Developmental Neuroscience
volume
42
pages
46 - 58
publisher
Elsevier
external identifiers
  • pmid:25758423
  • wos:000353611800006
  • scopus:84925324969
ISSN
1873-474X
DOI
10.1016/j.ijdevneu.2015.03.002
language
English
LU publication?
yes
id
5ba48b1d-498d-408e-97ae-7c0e0d85a731 (old id 5264754)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25758423?dopt=Abstract
date added to LUP
2015-04-02 18:52:47
date last changed
2017-09-17 03:34:29
@article{5ba48b1d-498d-408e-97ae-7c0e0d85a731,
  abstract     = {PURPOSE: To examine the expression of interstitial extracellular matrix components and their role during retinal development.<br/><br>
MATERIAL AND METHODS: Fibronectin (FN), collagen IV (Coll IV) and laminin 5 (Lam 5) expression in rat retinas from developmental stages E17 to adult were studied. In addition, PN5 full-thickness retinas were cultured for 7 days with dispase, which selectively cleaves FN and Coll IV, at either 0.5U/ml or 5.0U/ml for 3 or 24h. Eyecups and retinal cultures were examined morphologically using hematoxylin and eosin staining and immunohistochemistry.<br/><br>
RESULTS: Coll IV, Lam 5 and FN were all transiently expressed in the interstitial matrix of the retinal layers during development. The retinal layers in dispase treated explants was severely disturbed in a dose and time dependent manner.<br/><br>
CONCLUSIONS: FN, Lam 5 and Coll IV, are present in the interstitial extracellular matrix during rat retinal development. Enzymatic cleavage of FN and Coll IV early in the lamination process disrupts the retinal layers implicating their pivotal role in this process.},
  author       = {Taylor, Linnéa and Arnér, Karin and Engelsberg, Karl and Ghosh, Fredrik},
  issn         = {1873-474X},
  language     = {eng},
  pages        = {46--58},
  publisher    = {Elsevier},
  series       = {International Journal of Developmental Neuroscience},
  title        = {Scaffolding the retina: The interstitial extracellular matrix during rat retinal development.},
  url          = {http://dx.doi.org/10.1016/j.ijdevneu.2015.03.002},
  volume       = {42},
  year         = {2015},
}