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Analysis of Programmed Cell Death and Senescence Markers in the Developing Retina of an Altricial Bird Species

Álvarez-Hernán, Guadalupe LU ; de Mera-Rodríguez, José Antonio ; Hernández-Núñez, Ismael ; Marzal, Alfonso ; Gañán, Yolanda ; Martín-Partido, Gervasio ; Rodríguez-León, Joaquín and Francisco-Morcillo, Javier (2021) In Cells 10(3).
Abstract

This study shows the distribution patterns of apoptotic cells and biomarkers of cellular senescence during the ontogeny of the retina in the zebra finch (T. guttata). Neurogenesis in this altricial bird species is intense in the retina at perinatal and post-hatching stages, as opposed to precocial bird species in which retinogenesis occurs entirely during the embryonic period. Various phases of programmed cell death (PCD) were distinguishable in the T. guttata visual system. These included areas of PCD in the central region of the neuroretina at the stages of optic cup morphogenesis, and in the sub-optic necrotic centers (St15-20). A small focus of early neural PCD was detected in the neuroblastic layer, dorsal to the optic nerve head,... (More)

This study shows the distribution patterns of apoptotic cells and biomarkers of cellular senescence during the ontogeny of the retina in the zebra finch (T. guttata). Neurogenesis in this altricial bird species is intense in the retina at perinatal and post-hatching stages, as opposed to precocial bird species in which retinogenesis occurs entirely during the embryonic period. Various phases of programmed cell death (PCD) were distinguishable in the T. guttata visual system. These included areas of PCD in the central region of the neuroretina at the stages of optic cup morphogenesis, and in the sub-optic necrotic centers (St15-20). A small focus of early neural PCD was detected in the neuroblastic layer, dorsal to the optic nerve head, coinciding with the appearance of the first differentiated neuroblasts (St24-St25). There were sparse pyknotic bodies in the non-laminated retina between St26 and St37. An intense wave of neurotrophic PCD was detected in the laminated retina between St42 and P8, the last post-hatching stage included in the present study. PCD was absent from the photoreceptor layer. Phagocytic activity was also detected in Müller cells during the wave of neurotrophic PCD. With regard to the chronotopographical staining patterns of senescence biomarkers, there was strong parallelism between the SA-β-GAL signal and p21 immunoreactivity in both the undifferentiated and the laminated retina, coinciding in the cell body of differentiated neurons. In contrast, no correlation was found between SA-β-GAL activity and the distribution of TUNEL-positive cells in the developing tissue.

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author
; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Apoptosis/genetics, Birds, Cellular Senescence/physiology, Embryonic Development/physiology, Finches, Retina/physiopathology
in
Cells
volume
10
issue
3
article number
504
publisher
MDPI AG
external identifiers
  • pmid:33652964
  • scopus:85102324971
ISSN
2073-4409
DOI
10.3390/cells10030504
language
English
LU publication?
no
id
527712be-b66d-4a96-929a-7ff91bd1e7f1
date added to LUP
2021-12-15 14:04:15
date last changed
2024-06-15 00:33:04
@article{527712be-b66d-4a96-929a-7ff91bd1e7f1,
  abstract     = {{<p>This study shows the distribution patterns of apoptotic cells and biomarkers of cellular senescence during the ontogeny of the retina in the zebra finch (T. guttata). Neurogenesis in this altricial bird species is intense in the retina at perinatal and post-hatching stages, as opposed to precocial bird species in which retinogenesis occurs entirely during the embryonic period. Various phases of programmed cell death (PCD) were distinguishable in the T. guttata visual system. These included areas of PCD in the central region of the neuroretina at the stages of optic cup morphogenesis, and in the sub-optic necrotic centers (St15-20). A small focus of early neural PCD was detected in the neuroblastic layer, dorsal to the optic nerve head, coinciding with the appearance of the first differentiated neuroblasts (St24-St25). There were sparse pyknotic bodies in the non-laminated retina between St26 and St37. An intense wave of neurotrophic PCD was detected in the laminated retina between St42 and P8, the last post-hatching stage included in the present study. PCD was absent from the photoreceptor layer. Phagocytic activity was also detected in Müller cells during the wave of neurotrophic PCD. With regard to the chronotopographical staining patterns of senescence biomarkers, there was strong parallelism between the SA-β-GAL signal and p21 immunoreactivity in both the undifferentiated and the laminated retina, coinciding in the cell body of differentiated neurons. In contrast, no correlation was found between SA-β-GAL activity and the distribution of TUNEL-positive cells in the developing tissue.</p>}},
  author       = {{Álvarez-Hernán, Guadalupe and de Mera-Rodríguez, José Antonio and Hernández-Núñez, Ismael and Marzal, Alfonso and Gañán, Yolanda and Martín-Partido, Gervasio and Rodríguez-León, Joaquín and Francisco-Morcillo, Javier}},
  issn         = {{2073-4409}},
  keywords     = {{Animals; Apoptosis/genetics; Birds; Cellular Senescence/physiology; Embryonic Development/physiology; Finches; Retina/physiopathology}},
  language     = {{eng}},
  month        = {{02}},
  number       = {{3}},
  publisher    = {{MDPI AG}},
  series       = {{Cells}},
  title        = {{Analysis of Programmed Cell Death and Senescence Markers in the Developing Retina of an Altricial Bird Species}},
  url          = {{http://dx.doi.org/10.3390/cells10030504}},
  doi          = {{10.3390/cells10030504}},
  volume       = {{10}},
  year         = {{2021}},
}