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Characterizing nanoparticles in complex biological media and physiological fluids with depolarized dynamic light scattering

Balog, S.; Rodriguez-Lorenzo, L.; Monnier, C. A.; Obiols-Rabasa, Marc LU ; Rothen-Rutishauser, B.; Schurtenberger, Peter LU and Petri-Fink, A. (2015) In Nanoscale 7(14). p.5991-5997
Abstract
Light scattering is one of the few techniques available to adequately characterize suspended nanoparticles (NPs) in real time and in situ. However, when it comes to NPs in multicomponent and optically complex aqueous matrices - such as biological media and physiological fluids - light scattering suffers from lack of selectivity, as distinguishing the relevant optical signals from the irrelevant ones is very challenging. We meet this challenge by building on depolarized scattering: Unwanted signals from the matrix are completely suppressed. This approach yields information with an unprecedented signal-to-noise ratio in favour of the NPs and NP-biomolecule corona complexes, which in turn opens the frontier to scattering-based studies... (More)
Light scattering is one of the few techniques available to adequately characterize suspended nanoparticles (NPs) in real time and in situ. However, when it comes to NPs in multicomponent and optically complex aqueous matrices - such as biological media and physiological fluids - light scattering suffers from lack of selectivity, as distinguishing the relevant optical signals from the irrelevant ones is very challenging. We meet this challenge by building on depolarized scattering: Unwanted signals from the matrix are completely suppressed. This approach yields information with an unprecedented signal-to-noise ratio in favour of the NPs and NP-biomolecule corona complexes, which in turn opens the frontier to scattering-based studies addressing the behaviour of NPs in complex physiological/biological fluids. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nanoscale
volume
7
issue
14
pages
5991 - 5997
publisher
Royal Society of Chemistry
external identifiers
  • wos:000351934700011
  • scopus:84961290408
ISSN
2040-3372
DOI
10.1039/c4nr06538g
language
English
LU publication?
yes
id
60cfcc1f-dd69-4e61-8bfd-d2cc52d0f165 (old id 5281642)
date added to LUP
2015-04-24 11:37:22
date last changed
2017-11-19 03:15:37
@article{60cfcc1f-dd69-4e61-8bfd-d2cc52d0f165,
  abstract     = {Light scattering is one of the few techniques available to adequately characterize suspended nanoparticles (NPs) in real time and in situ. However, when it comes to NPs in multicomponent and optically complex aqueous matrices - such as biological media and physiological fluids - light scattering suffers from lack of selectivity, as distinguishing the relevant optical signals from the irrelevant ones is very challenging. We meet this challenge by building on depolarized scattering: Unwanted signals from the matrix are completely suppressed. This approach yields information with an unprecedented signal-to-noise ratio in favour of the NPs and NP-biomolecule corona complexes, which in turn opens the frontier to scattering-based studies addressing the behaviour of NPs in complex physiological/biological fluids.},
  author       = {Balog, S. and Rodriguez-Lorenzo, L. and Monnier, C. A. and Obiols-Rabasa, Marc and Rothen-Rutishauser, B. and Schurtenberger, Peter and Petri-Fink, A.},
  issn         = {2040-3372},
  language     = {eng},
  number       = {14},
  pages        = {5991--5997},
  publisher    = {Royal Society of Chemistry},
  series       = {Nanoscale},
  title        = {Characterizing nanoparticles in complex biological media and physiological fluids with depolarized dynamic light scattering},
  url          = {http://dx.doi.org/10.1039/c4nr06538g},
  volume       = {7},
  year         = {2015},
}