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Enrichment of antibiotic resistance genes within bacteriophage populations in saliva samples from individuals undergoing oral antibiotic treatments

Andersson, Tilde LU ; Makenga, Geofrey ; Francis, Filbert ; Minja, Daniel T.R. ; Overballe-Petersen, Soren ; Tang, Man Hung Eric LU ; Fuursted, Kurt ; Baraka, Vito and Lood, Rolf LU (2022) In Frontiers in Microbiology 13.
Abstract

Spread of antibiotic resistance is a significant challenge for our modern health care system, and even more so in developing countries with higher prevalence of both infections and resistant bacteria. Faulty usage of antibiotics has been pinpointed as a driving factor in spread of resistant bacteria through selective pressure. However, horizontal gene transfer mediated through bacteriophages may also play an important role in this spread. In a cohort of Tanzanian patients suffering from bacterial infections, we demonstrate significant differences in the oral microbial diversity between infected and non-infected individuals, as well as before and after oral antibiotics treatment. Further, the resistome carried both by bacteria and... (More)

Spread of antibiotic resistance is a significant challenge for our modern health care system, and even more so in developing countries with higher prevalence of both infections and resistant bacteria. Faulty usage of antibiotics has been pinpointed as a driving factor in spread of resistant bacteria through selective pressure. However, horizontal gene transfer mediated through bacteriophages may also play an important role in this spread. In a cohort of Tanzanian patients suffering from bacterial infections, we demonstrate significant differences in the oral microbial diversity between infected and non-infected individuals, as well as before and after oral antibiotics treatment. Further, the resistome carried both by bacteria and bacteriophages vary significantly, with blaCTX-M1 resistance genes being mobilized and enriched within phage populations. This may impact how we consider spread of resistance in a biological context, as well in terms of treatment regimes.

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
antibiotic resistance, antibiotic treatment, Bacteriophage, microbiota, saliva
in
Frontiers in Microbiology
volume
13
article number
1049110
publisher
Frontiers Media S. A.
external identifiers
  • pmid:36425042
  • scopus:85142225368
ISSN
1664-302X
DOI
10.3389/fmicb.2022.1049110
language
English
LU publication?
yes
id
5289ee80-906f-40f0-a4d8-f35de0dc6941
date added to LUP
2023-01-03 14:17:39
date last changed
2024-05-02 12:08:15
@article{5289ee80-906f-40f0-a4d8-f35de0dc6941,
  abstract     = {{<p>Spread of antibiotic resistance is a significant challenge for our modern health care system, and even more so in developing countries with higher prevalence of both infections and resistant bacteria. Faulty usage of antibiotics has been pinpointed as a driving factor in spread of resistant bacteria through selective pressure. However, horizontal gene transfer mediated through bacteriophages may also play an important role in this spread. In a cohort of Tanzanian patients suffering from bacterial infections, we demonstrate significant differences in the oral microbial diversity between infected and non-infected individuals, as well as before and after oral antibiotics treatment. Further, the resistome carried both by bacteria and bacteriophages vary significantly, with bla<sub>CTX-M1</sub> resistance genes being mobilized and enriched within phage populations. This may impact how we consider spread of resistance in a biological context, as well in terms of treatment regimes.</p>}},
  author       = {{Andersson, Tilde and Makenga, Geofrey and Francis, Filbert and Minja, Daniel T.R. and Overballe-Petersen, Soren and Tang, Man Hung Eric and Fuursted, Kurt and Baraka, Vito and Lood, Rolf}},
  issn         = {{1664-302X}},
  keywords     = {{antibiotic resistance; antibiotic treatment; Bacteriophage; microbiota; saliva}},
  language     = {{eng}},
  month        = {{11}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Microbiology}},
  title        = {{Enrichment of antibiotic resistance genes within bacteriophage populations in saliva samples from individuals undergoing oral antibiotic treatments}},
  url          = {{http://dx.doi.org/10.3389/fmicb.2022.1049110}},
  doi          = {{10.3389/fmicb.2022.1049110}},
  volume       = {{13}},
  year         = {{2022}},
}