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Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Production by a Moderate Halophile Yangia sp ND199 Using Glycerol as a Carbon Source

Van-Thuoc, Doan; Huu-Phong, Tran; Minh-Khuong, Dang and Hatti-Kaul, Rajni LU (2015) In Applied Biochemistry and Biotechnology 175(6). p.3120-3132
Abstract
Yangia sp. ND199, a moderate halophile isolated from mangrove soil sample in Vietnam, was found to accumulate poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from unrelated carbon sources in a medium with 4.5 % (w/v) NaCl. Cultivation with glycerol as carbon source and yeast extract as nitrogen source resulted in maximum cell dry weight of 5.7 g/l and PHBV content of 52.8 wt% (containing 2.9 mol% of 3HV) after 40 h. The 3HV content of the PHBV was the highest during initial stages of copolymer production and decreased with increase in the copolymer amount with time, but was not affected by changing the pH of the culture medium. Only homopolymer poly(3-hydroxybutyrate) was synthesized when monosodium glutamate was used as the nitrogen... (More)
Yangia sp. ND199, a moderate halophile isolated from mangrove soil sample in Vietnam, was found to accumulate poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from unrelated carbon sources in a medium with 4.5 % (w/v) NaCl. Cultivation with glycerol as carbon source and yeast extract as nitrogen source resulted in maximum cell dry weight of 5.7 g/l and PHBV content of 52.8 wt% (containing 2.9 mol% of 3HV) after 40 h. The 3HV content of the PHBV was the highest during initial stages of copolymer production and decreased with increase in the copolymer amount with time, but was not affected by changing the pH of the culture medium. Only homopolymer poly(3-hydroxybutyrate) was synthesized when monosodium glutamate was used as the nitrogen source. Fed-batch cultivation of Yangia sp. ND199 with glycerol and yeast extract gave PHBV content and productivity of 53.2 wt% and 0.44 g/l/h, respectively, which were reduced to 40.6 wt% and 0.25 g/l/h, respectively, with crude glycerol as carbon source. Both the copolymer content and productivity were improved to 56 wt% and 0.61 g/l/h, respectively, by using 1:1 mixture of crude glycerol and high fructose corn syrup. This is the first report of PHBV production by a wild-type halophilic bacterium using glycerol as carbon source. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Yangia sp ND199, Poly(3-hydroxybutyrate-co-3-hydroxyvalerate), Glycerol, Carbon source, 3HV content
in
Applied Biochemistry and Biotechnology
volume
175
issue
6
pages
3120 - 3132
publisher
Humana Press
external identifiers
  • wos:000350881100027
  • scopus:84924071730
ISSN
1559-0291
DOI
10.1007/s12010-015-1479-4
language
English
LU publication?
yes
id
94ab3ed0-10c6-47e5-bdd9-50179e896d77 (old id 5293690)
date added to LUP
2015-04-24 14:43:47
date last changed
2017-10-01 03:12:41
@article{94ab3ed0-10c6-47e5-bdd9-50179e896d77,
  abstract     = {Yangia sp. ND199, a moderate halophile isolated from mangrove soil sample in Vietnam, was found to accumulate poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from unrelated carbon sources in a medium with 4.5 % (w/v) NaCl. Cultivation with glycerol as carbon source and yeast extract as nitrogen source resulted in maximum cell dry weight of 5.7 g/l and PHBV content of 52.8 wt% (containing 2.9 mol% of 3HV) after 40 h. The 3HV content of the PHBV was the highest during initial stages of copolymer production and decreased with increase in the copolymer amount with time, but was not affected by changing the pH of the culture medium. Only homopolymer poly(3-hydroxybutyrate) was synthesized when monosodium glutamate was used as the nitrogen source. Fed-batch cultivation of Yangia sp. ND199 with glycerol and yeast extract gave PHBV content and productivity of 53.2 wt% and 0.44 g/l/h, respectively, which were reduced to 40.6 wt% and 0.25 g/l/h, respectively, with crude glycerol as carbon source. Both the copolymer content and productivity were improved to 56 wt% and 0.61 g/l/h, respectively, by using 1:1 mixture of crude glycerol and high fructose corn syrup. This is the first report of PHBV production by a wild-type halophilic bacterium using glycerol as carbon source.},
  author       = {Van-Thuoc, Doan and Huu-Phong, Tran and Minh-Khuong, Dang and Hatti-Kaul, Rajni},
  issn         = {1559-0291},
  keyword      = {Yangia sp ND199,Poly(3-hydroxybutyrate-co-3-hydroxyvalerate),Glycerol,Carbon source,3HV content},
  language     = {eng},
  number       = {6},
  pages        = {3120--3132},
  publisher    = {Humana Press},
  series       = {Applied Biochemistry and Biotechnology},
  title        = {Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Production by a Moderate Halophile Yangia sp ND199 Using Glycerol as a Carbon Source},
  url          = {http://dx.doi.org/10.1007/s12010-015-1479-4},
  volume       = {175},
  year         = {2015},
}