Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Production by a Moderate Halophile Yangia sp ND199 Using Glycerol as a Carbon Source
(2015) In Applied Biochemistry and Biotechnology 175(6). p.3120-3132- Abstract
- Yangia sp. ND199, a moderate halophile isolated from mangrove soil sample in Vietnam, was found to accumulate poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from unrelated carbon sources in a medium with 4.5 % (w/v) NaCl. Cultivation with glycerol as carbon source and yeast extract as nitrogen source resulted in maximum cell dry weight of 5.7 g/l and PHBV content of 52.8 wt% (containing 2.9 mol% of 3HV) after 40 h. The 3HV content of the PHBV was the highest during initial stages of copolymer production and decreased with increase in the copolymer amount with time, but was not affected by changing the pH of the culture medium. Only homopolymer poly(3-hydroxybutyrate) was synthesized when monosodium glutamate was used as the nitrogen... (More)
- Yangia sp. ND199, a moderate halophile isolated from mangrove soil sample in Vietnam, was found to accumulate poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from unrelated carbon sources in a medium with 4.5 % (w/v) NaCl. Cultivation with glycerol as carbon source and yeast extract as nitrogen source resulted in maximum cell dry weight of 5.7 g/l and PHBV content of 52.8 wt% (containing 2.9 mol% of 3HV) after 40 h. The 3HV content of the PHBV was the highest during initial stages of copolymer production and decreased with increase in the copolymer amount with time, but was not affected by changing the pH of the culture medium. Only homopolymer poly(3-hydroxybutyrate) was synthesized when monosodium glutamate was used as the nitrogen source. Fed-batch cultivation of Yangia sp. ND199 with glycerol and yeast extract gave PHBV content and productivity of 53.2 wt% and 0.44 g/l/h, respectively, which were reduced to 40.6 wt% and 0.25 g/l/h, respectively, with crude glycerol as carbon source. Both the copolymer content and productivity were improved to 56 wt% and 0.61 g/l/h, respectively, by using 1:1 mixture of crude glycerol and high fructose corn syrup. This is the first report of PHBV production by a wild-type halophilic bacterium using glycerol as carbon source. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5293690
- author
- Van-Thuoc, Doan ; Huu-Phong, Tran ; Minh-Khuong, Dang and Hatti-Kaul, Rajni LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Yangia sp ND199, Poly(3-hydroxybutyrate-co-3-hydroxyvalerate), Glycerol, Carbon source, 3HV content
- in
- Applied Biochemistry and Biotechnology
- volume
- 175
- issue
- 6
- pages
- 3120 - 3132
- publisher
- Humana Press
- external identifiers
-
- wos:000350881100027
- scopus:84924071730
- ISSN
- 1559-0291
- DOI
- 10.1007/s12010-015-1479-4
- language
- English
- LU publication?
- yes
- id
- 94ab3ed0-10c6-47e5-bdd9-50179e896d77 (old id 5293690)
- date added to LUP
- 2016-04-01 10:19:51
- date last changed
- 2022-04-27 20:55:58
@article{94ab3ed0-10c6-47e5-bdd9-50179e896d77,
abstract = {{Yangia sp. ND199, a moderate halophile isolated from mangrove soil sample in Vietnam, was found to accumulate poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from unrelated carbon sources in a medium with 4.5 % (w/v) NaCl. Cultivation with glycerol as carbon source and yeast extract as nitrogen source resulted in maximum cell dry weight of 5.7 g/l and PHBV content of 52.8 wt% (containing 2.9 mol% of 3HV) after 40 h. The 3HV content of the PHBV was the highest during initial stages of copolymer production and decreased with increase in the copolymer amount with time, but was not affected by changing the pH of the culture medium. Only homopolymer poly(3-hydroxybutyrate) was synthesized when monosodium glutamate was used as the nitrogen source. Fed-batch cultivation of Yangia sp. ND199 with glycerol and yeast extract gave PHBV content and productivity of 53.2 wt% and 0.44 g/l/h, respectively, which were reduced to 40.6 wt% and 0.25 g/l/h, respectively, with crude glycerol as carbon source. Both the copolymer content and productivity were improved to 56 wt% and 0.61 g/l/h, respectively, by using 1:1 mixture of crude glycerol and high fructose corn syrup. This is the first report of PHBV production by a wild-type halophilic bacterium using glycerol as carbon source.}},
author = {{Van-Thuoc, Doan and Huu-Phong, Tran and Minh-Khuong, Dang and Hatti-Kaul, Rajni}},
issn = {{1559-0291}},
keywords = {{Yangia sp ND199; Poly(3-hydroxybutyrate-co-3-hydroxyvalerate); Glycerol; Carbon source; 3HV content}},
language = {{eng}},
number = {{6}},
pages = {{3120--3132}},
publisher = {{Humana Press}},
series = {{Applied Biochemistry and Biotechnology}},
title = {{Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Production by a Moderate Halophile Yangia sp ND199 Using Glycerol as a Carbon Source}},
url = {{http://dx.doi.org/10.1007/s12010-015-1479-4}},
doi = {{10.1007/s12010-015-1479-4}},
volume = {{175}},
year = {{2015}},
}