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Automation and Experience of Controlled Crystal Dehydration: Results from the European Synchrotron HC1 Collaboration

Bowler, Matthew W.; Mueller, Uwe; Weiss, Manfred S.; Sanchez-Weatherby, Juan; Sorensen, Thomas L-M.; Thunnissen, Marjolein LU ; Ursby, Thomas LU ; Gobbo, Alexandre; Russi, Silvia and Bowler, Michael G., et al. (2015) In Crystal Growth & Design 15(3). p.1043-1054
Abstract
Controlled dehydration of macromolecular crystals can lead to significant improvements in crystalline order, which often manifests itself in higher diffraction quality. Devices that can accurately control the humidity surrounding crystals on a beamline have led to this technique being increasingly adopted as experiments become easier and more reproducible. However, these experiments are often carried out by trial and error, and in order to facilitate and streamline them four European synchrotrons have established a collaboration around the HC1b dehydration device. The MAX IV Laboratory, Diamond Light Source, BESSY II, and the EMBL Grenoble Outstation/ESRF have pooled information gathered from user experiments, and on the use of the device,... (More)
Controlled dehydration of macromolecular crystals can lead to significant improvements in crystalline order, which often manifests itself in higher diffraction quality. Devices that can accurately control the humidity surrounding crystals on a beamline have led to this technique being increasingly adopted as experiments become easier and more reproducible. However, these experiments are often carried out by trial and error, and in order to facilitate and streamline them four European synchrotrons have established a collaboration around the HC1b dehydration device. The MAX IV Laboratory, Diamond Light Source, BESSY II, and the EMBL Grenoble Outstation/ESRF have pooled information gathered from user experiments, and on the use of the device, to propose a set of guidelines for these experiments. Here, we present the status and automation of the installations, advice on how best to perform experiments using the device, and an analysis of successful experiments that begins to show some trends in the type of protocols required by some systems. The dehydration methods shown are applicable to any device that allows control of the relative humidity of the air surrounding a macromolecular crystal. (Less)
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Contribution to journal
publication status
published
subject
in
Crystal Growth & Design
volume
15
issue
3
pages
1043 - 1054
publisher
The American Chemical Society
external identifiers
  • wos:000350614400006
  • scopus:84924336744
ISSN
1528-7483
DOI
10.1021/cg500890r
language
English
LU publication?
yes
id
fd2701a8-65c6-431a-88e8-0233a2224011 (old id 5293725)
date added to LUP
2015-04-24 14:57:28
date last changed
2017-08-20 03:10:55
@article{fd2701a8-65c6-431a-88e8-0233a2224011,
  abstract     = {Controlled dehydration of macromolecular crystals can lead to significant improvements in crystalline order, which often manifests itself in higher diffraction quality. Devices that can accurately control the humidity surrounding crystals on a beamline have led to this technique being increasingly adopted as experiments become easier and more reproducible. However, these experiments are often carried out by trial and error, and in order to facilitate and streamline them four European synchrotrons have established a collaboration around the HC1b dehydration device. The MAX IV Laboratory, Diamond Light Source, BESSY II, and the EMBL Grenoble Outstation/ESRF have pooled information gathered from user experiments, and on the use of the device, to propose a set of guidelines for these experiments. Here, we present the status and automation of the installations, advice on how best to perform experiments using the device, and an analysis of successful experiments that begins to show some trends in the type of protocols required by some systems. The dehydration methods shown are applicable to any device that allows control of the relative humidity of the air surrounding a macromolecular crystal.},
  author       = {Bowler, Matthew W. and Mueller, Uwe and Weiss, Manfred S. and Sanchez-Weatherby, Juan and Sorensen, Thomas L-M. and Thunnissen, Marjolein and Ursby, Thomas and Gobbo, Alexandre and Russi, Silvia and Bowler, Michael G. and Brockhauser, Sandor and Svensson, Olof and Cipriani, Florent},
  issn         = {1528-7483},
  language     = {eng},
  number       = {3},
  pages        = {1043--1054},
  publisher    = {The American Chemical Society},
  series       = {Crystal Growth & Design},
  title        = {Automation and Experience of Controlled Crystal Dehydration: Results from the European Synchrotron HC1 Collaboration},
  url          = {http://dx.doi.org/10.1021/cg500890r},
  volume       = {15},
  year         = {2015},
}