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Genotoxic effects of daily personal exposure to particle mass and number concentrations on buccal cells

de Almeida, Daniela S.; da Costa, Silvano César; Ribeiro, Marcos; Moreira, Camila A.B.; Beal, Alexandra; Squizzato, Rafaela; Rudke, Anderson Paulo; Rafee, Sameh Adib Abou; Martins, Jorge A. LU and Palioto, Graciana Freitas, et al. (2018) In Atmospheric Environment 176. p.148-157
Abstract

The aim of this study is to assess personal exposure to Particle Number Concentrations (PNC) in four size ranges between 0.3 and 10 μm, and particulate matter (PM1; PM2.5; PM4; PM10) in order to evaluate possible genotoxic effects through a comet assay in buccal cells. A convenience cohort of 30 individuals from a Brazilian medium-sized city was selected. These individuals aged between 20 and 61 and worked in typical job categories (i.e., administrative, commerce, education, general services and transport). They were recruited to perform personal exposure measurements during their typical daily routine activities, totaling 240 h of sampling. The 8-h average mass concentrations in air for... (More)

The aim of this study is to assess personal exposure to Particle Number Concentrations (PNC) in four size ranges between 0.3 and 10 μm, and particulate matter (PM1; PM2.5; PM4; PM10) in order to evaluate possible genotoxic effects through a comet assay in buccal cells. A convenience cohort of 30 individuals from a Brazilian medium-sized city was selected. These individuals aged between 20 and 61 and worked in typical job categories (i.e., administrative, commerce, education, general services and transport). They were recruited to perform personal exposure measurements during their typical daily routine activities, totaling 240 h of sampling. The 8-h average mass concentrations in air for volunteers ranged from 2.4 to 31.8 μg m−3 for PM1, 4.2–45.1 μg m−3 for PM2.5, 7.9–66.1 μg m−3 for PM4 and from 23.1 to 131.7 μg m−3 for PM10. The highest PNC variation was found for 0.3–0.5 range, between 14 and 181 particles cm−3, 1 to 14 particles cm−3 for the 0.5–1.0 range, 0.2 to 2 particles cm−3 for the 1.0–2.5 range, and 0.06 to 0.7 particles cm−3 for the 2.5–10 range. Volunteers in the ‘education’ category experienced the lowest inhaled dose of PM2.5 as opposed to those involved in ‘commercial‘ activities with the highest doses for PM10 (1.63 μg kg−1 h−1) and PM2.5 (0.61 μg kg−1 h−1). The predominant cause for these high doses was associated with the proximity of the workplace to the street and vehicle traffic. The comet assay performed in buccal cells indicated that the volunteers in ‘commerce’ category experienced the highest damage to their DeoxyriboNucleic Acid (DNA) compared with the control category (i.e. ‘education’). These results indicate the variability in personal exposure of the volunteers in different groups, and the potential damage to DNA was much higher for those spending time in close proximity to the vehicle sources (e.g. commercial services) leading to exposure to a higher fraction of fine particles. This study builds understanding on the exposure of people in different job categories, and provide policy makers with useful information to tackle this neglected issue.

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publication status
published
subject
keywords
Air pollution, DNA damage, Fine particulate matter, Personal exposure
in
Atmospheric Environment
volume
176
pages
10 pages
publisher
Elsevier
external identifiers
  • scopus:85039787001
ISSN
1352-2310
DOI
10.1016/j.atmosenv.2017.12.021
language
English
LU publication?
yes
id
529a0de3-1430-4060-ad81-63481b4f31cb
date added to LUP
2018-01-09 09:24:19
date last changed
2018-08-12 04:41:54
@article{529a0de3-1430-4060-ad81-63481b4f31cb,
  abstract     = {<p>The aim of this study is to assess personal exposure to Particle Number Concentrations (PNC) in four size ranges between 0.3 and 10 μm, and particulate matter (PM<sub>1</sub>; PM<sub>2.5</sub>; PM<sub>4</sub>; PM<sub>10</sub>) in order to evaluate possible genotoxic effects through a comet assay in buccal cells. A convenience cohort of 30 individuals from a Brazilian medium-sized city was selected. These individuals aged between 20 and 61 and worked in typical job categories (i.e., administrative, commerce, education, general services and transport). They were recruited to perform personal exposure measurements during their typical daily routine activities, totaling 240 h of sampling. The 8-h average mass concentrations in air for volunteers ranged from 2.4 to 31.8 μg m<sup>−3</sup> for PM<sub>1</sub>, 4.2–45.1 μg m<sup>−3</sup> for PM<sub>2.5</sub>, 7.9–66.1 μg m<sup>−3</sup> for PM<sub>4</sub> and from 23.1 to 131.7 μg m<sup>−3</sup> for PM<sub>10</sub>. The highest PNC variation was found for 0.3–0.5 range, between 14 and 181 particles cm<sup>−3</sup>, 1 to 14 particles cm<sup>−3</sup> for the 0.5–1.0 range, 0.2 to 2 particles cm<sup>−3</sup> for the 1.0–2.5 range, and 0.06 to 0.7 particles cm<sup>−3</sup> for the 2.5–10 range. Volunteers in the ‘education’ category experienced the lowest inhaled dose of PM<sub>2.5</sub> as opposed to those involved in ‘commercial‘ activities with the highest doses for PM<sub>10</sub> (1.63 μg kg<sup>−1</sup> h<sup>−1</sup>) and PM<sub>2.5</sub> (0.61 μg kg<sup>−1</sup> h<sup>−1</sup>). The predominant cause for these high doses was associated with the proximity of the workplace to the street and vehicle traffic. The comet assay performed in buccal cells indicated that the volunteers in ‘commerce’ category experienced the highest damage to their DeoxyriboNucleic Acid (DNA) compared with the control category (i.e. ‘education’). These results indicate the variability in personal exposure of the volunteers in different groups, and the potential damage to DNA was much higher for those spending time in close proximity to the vehicle sources (e.g. commercial services) leading to exposure to a higher fraction of fine particles. This study builds understanding on the exposure of people in different job categories, and provide policy makers with useful information to tackle this neglected issue.</p>},
  author       = {de Almeida, Daniela S. and da Costa, Silvano César and Ribeiro, Marcos and Moreira, Camila A.B. and Beal, Alexandra and Squizzato, Rafaela and Rudke, Anderson Paulo and Rafee, Sameh Adib Abou and Martins, Jorge A. and Palioto, Graciana Freitas and Kumar, Prashant and Martins, Leila D.},
  issn         = {1352-2310},
  keyword      = {Air pollution,DNA damage,Fine particulate matter,Personal exposure},
  language     = {eng},
  month        = {03},
  pages        = {148--157},
  publisher    = {Elsevier},
  series       = {Atmospheric Environment},
  title        = {Genotoxic effects of daily personal exposure to particle mass and number concentrations on buccal cells},
  url          = {http://dx.doi.org/10.1016/j.atmosenv.2017.12.021},
  volume       = {176},
  year         = {2018},
}