Nonaqueous capillary electrophoresis for analysis of the ethanol consumption biomarker phosphatidylethanol.
(2008) In Electrophoresis 29(8). p.1667-1671- Abstract
- Nonaqueous CE (NACE) methodology was developed for the separation and determination of phosphatidylethanol (Peth), a new biomarker of ethanol intake. Peth is an abnormal phospholipid formed in cell membranes only in the presence of ethanol, via the transphosphatidylation reaction of phospholipase D. The NACE separation medium consisted of 80 mM ammonium acetate in 50% ACN, 33% 2-propanol, 12% hexane and 5% methanol. A stacking effect was obtained by reducing the concentration of ammonium acetate in the separation medium for all injected samples. The LOD was estimated to 1 muM (5.6 fmol) of Peth with conventional UV detection, equalling 0.4 mumol/L blood. Peth was successfully determined in extracts of human blood samples. Separation of... (More)
- Nonaqueous CE (NACE) methodology was developed for the separation and determination of phosphatidylethanol (Peth), a new biomarker of ethanol intake. Peth is an abnormal phospholipid formed in cell membranes only in the presence of ethanol, via the transphosphatidylation reaction of phospholipase D. The NACE separation medium consisted of 80 mM ammonium acetate in 50% ACN, 33% 2-propanol, 12% hexane and 5% methanol. A stacking effect was obtained by reducing the concentration of ammonium acetate in the separation medium for all injected samples. The LOD was estimated to 1 muM (5.6 fmol) of Peth with conventional UV detection, equalling 0.4 mumol/L blood. Peth was successfully determined in extracts of human blood samples. Separation of Peth from other blood lipids in the lipid extract sample was performed in 5 min. The method facilitates smaller sample volumes and performs about ten times faster compared to earlier chromatographical methods. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1147872
- author
- Varga, Arthur LU and Nilsson, Staffan LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Electrophoresis
- volume
- 29
- issue
- 8
- pages
- 1667 - 1671
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000255703100011
- pmid:18383031
- scopus:42649089187
- ISSN
- 0173-0835
- DOI
- 10.1002/elps.200700548
- language
- English
- LU publication?
- yes
- id
- 52c0c30a-aa70-4746-ac46-25ab9235249d (old id 1147872)
- date added to LUP
- 2016-04-01 11:56:51
- date last changed
- 2022-01-26 20:32:55
@article{52c0c30a-aa70-4746-ac46-25ab9235249d,
abstract = {{Nonaqueous CE (NACE) methodology was developed for the separation and determination of phosphatidylethanol (Peth), a new biomarker of ethanol intake. Peth is an abnormal phospholipid formed in cell membranes only in the presence of ethanol, via the transphosphatidylation reaction of phospholipase D. The NACE separation medium consisted of 80 mM ammonium acetate in 50% ACN, 33% 2-propanol, 12% hexane and 5% methanol. A stacking effect was obtained by reducing the concentration of ammonium acetate in the separation medium for all injected samples. The LOD was estimated to 1 muM (5.6 fmol) of Peth with conventional UV detection, equalling 0.4 mumol/L blood. Peth was successfully determined in extracts of human blood samples. Separation of Peth from other blood lipids in the lipid extract sample was performed in 5 min. The method facilitates smaller sample volumes and performs about ten times faster compared to earlier chromatographical methods.}},
author = {{Varga, Arthur and Nilsson, Staffan}},
issn = {{0173-0835}},
language = {{eng}},
number = {{8}},
pages = {{1667--1671}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Electrophoresis}},
title = {{Nonaqueous capillary electrophoresis for analysis of the ethanol consumption biomarker phosphatidylethanol.}},
url = {{http://dx.doi.org/10.1002/elps.200700548}},
doi = {{10.1002/elps.200700548}},
volume = {{29}},
year = {{2008}},
}