Functions of creatine kinase isoenzymes in spermatozoa
Kaldis, Philipp LU
; Kamp, Günter
; Piendl, Thomas
and Wallimann, Theo
(1997)
In Advances in Developmental Biology
5(C).
p.275-312
- Abstract
Spermatozoa have a defined function, that is, the fertilization of an egg. To achieve this goal, spermatozoa have to move actively toward the egg and inject the male pronucleus into the egg. Therefore, sperm motility is one of the crucial determinants for male fertility. Surprisingly, however, structure and energy metabolism can differ substantially between spermatozoa from different species. Here, we report on the presence of high-energy guanidino compounds and the presence of guanidino kinases in spermatozoa of different animal species, focusing mainly on creatine kinase (CK) isoenzymes. Spermatozoa from lugworm, sea urchin, rooster and a number of mammals, including man, contain a cytosolic CK isoenzyme, in most cases brain-type... (More)
Spermatozoa have a defined function, that is, the fertilization of an egg. To achieve this goal, spermatozoa have to move actively toward the egg and inject the male pronucleus into the egg. Therefore, sperm motility is one of the crucial determinants for male fertility. Surprisingly, however, structure and energy metabolism can differ substantially between spermatozoa from different species. Here, we report on the presence of high-energy guanidino compounds and the presence of guanidino kinases in spermatozoa of different animal species, focusing mainly on creatine kinase (CK) isoenzymes. Spermatozoa from lugworm, sea urchin, rooster and a number of mammals, including man, contain a cytosolic CK isoenzyme, in most cases brain-type B-CK, localized to the sperm tail. In addition, at least one mitochondrial CK isoenzyme was identified and specifically localized in the mitochondria containing midpiece. Specific inhibition of CK was shown to abolish flagellar movement in the distal third of the sperm tail from sea urchin. These experiments demonstrate in an in vivo cell model system the physiological importance of the CK isoenzymes participating in a phospho-creatine shuttle to overcome the diffusional limitations of adenosine nucleotides along the sperm tail. By comparison, the relative importance of CK for sperm function may range from absolutely essential, in sea urchin and rooster, to marginal, in some mammals, some of which do not contain CK in their sperms at all, for example, bull and boar. In the latter case, however, systems like fermentation and/or the adeny late kinase system compensate for the lack of CK and/or take over the function of CK.
(Less)
- author
- Kaldis, Philipp
LU
; Kamp, Günter
; Piendl, Thomas
and Wallimann, Theo
- publishing date
- 1997-12-01
- type
- Contribution to journal
- publication status
- published
- in
- Advances in Developmental Biology
- volume
- 5
- issue
- C
- pages
- 38 pages
- publisher
- JAI Press
- external identifiers
-
- scopus:0000091709
- ISSN
- 1566-3116
- DOI
- 10.1016/S1566-3116(08)60040-7
- language
- English
- LU publication?
- no
- id
- 52d81e67-ede8-4c06-b59c-273fd5f67305
- date added to LUP
- 2019-09-18 14:36:37
- date last changed
- 2022-02-01 00:51:53
@article{52d81e67-ede8-4c06-b59c-273fd5f67305,
abstract = {{<p>Spermatozoa have a defined function, that is, the fertilization of an egg. To achieve this goal, spermatozoa have to move actively toward the egg and inject the male pronucleus into the egg. Therefore, sperm motility is one of the crucial determinants for male fertility. Surprisingly, however, structure and energy metabolism can differ substantially between spermatozoa from different species. Here, we report on the presence of high-energy guanidino compounds and the presence of guanidino kinases in spermatozoa of different animal species, focusing mainly on creatine kinase (CK) isoenzymes. Spermatozoa from lugworm, sea urchin, rooster and a number of mammals, including man, contain a cytosolic CK isoenzyme, in most cases brain-type B-CK, localized to the sperm tail. In addition, at least one mitochondrial CK isoenzyme was identified and specifically localized in the mitochondria containing midpiece. Specific inhibition of CK was shown to abolish flagellar movement in the distal third of the sperm tail from sea urchin. These experiments demonstrate in an in vivo cell model system the physiological importance of the CK isoenzymes participating in a phospho-creatine shuttle to overcome the diffusional limitations of adenosine nucleotides along the sperm tail. By comparison, the relative importance of CK for sperm function may range from absolutely essential, in sea urchin and rooster, to marginal, in some mammals, some of which do not contain CK in their sperms at all, for example, bull and boar. In the latter case, however, systems like fermentation and/or the adeny late kinase system compensate for the lack of CK and/or take over the function of CK.</p>}},
author = {{Kaldis, Philipp and Kamp, Günter and Piendl, Thomas and Wallimann, Theo}},
issn = {{1566-3116}},
language = {{eng}},
month = {{12}},
number = {{C}},
pages = {{275--312}},
publisher = {{JAI Press}},
series = {{Advances in Developmental Biology}},
title = {{Functions of creatine kinase isoenzymes in spermatozoa}},
url = {{http://dx.doi.org/10.1016/S1566-3116(08)60040-7}},
doi = {{10.1016/S1566-3116(08)60040-7}},
volume = {{5}},
year = {{1997}},
}