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Iliac crest histomorphometry and skeletal heterogeneity in men

Tong, Xiaoyu; Burton, Inari S.; Jurvelin, Jukka S.; Isaksson, Hanna LU and Kröger, Heikki (2017) In Bone Reports 6. p.9-16
Abstract

Purpose The cortical characteristics of the iliac crest in male have rarely been investigated with quantitative histomorphometry. Also it is still unknown how cortical microarchitecture may vary between the iliac crest and fractures related sites at the proximal femur. We studied the microarchitecture of both external and internal cortices within the iliac crest, and compared the results with femoral neck and subtrochanteric femoral shaft sites. Methods Undecalcified histological sections of the iliac crest were obtained bicortically from cadavers (n = 20, aged 18–82 years, males). They were cut (7 μm) and stained using modified Masson-Goldner stain. Histomorphometric parameters of cortical bone were analysed with low (× 50) and high (×... (More)

Purpose The cortical characteristics of the iliac crest in male have rarely been investigated with quantitative histomorphometry. Also it is still unknown how cortical microarchitecture may vary between the iliac crest and fractures related sites at the proximal femur. We studied the microarchitecture of both external and internal cortices within the iliac crest, and compared the results with femoral neck and subtrochanteric femoral shaft sites. Methods Undecalcified histological sections of the iliac crest were obtained bicortically from cadavers (n = 20, aged 18–82 years, males). They were cut (7 μm) and stained using modified Masson-Goldner stain. Histomorphometric parameters of cortical bone were analysed with low (× 50) and high (× 100) magnification, after identifying cortical bone boundaries using our previously validated method. Within cortical bone area, only complete osteons with typical concentric lamellae and cement line were selected and measured. Results At the iliac crest, the mean cortical width of external cortex was higher than at the internal cortex (p < 0.001). Also, osteon structural parameters, e.g. mean osteonal perimeter, were higher in the external cortex (p < 0.05). In both external and internal cortices, pore number per cortical bone area was higher in young subjects (≤ 50 years) (p < 0.05) while mean pore perimeter was higher in the old subjects (> 50 years) (p < 0.05). Several cortical parameters (e.g. osteon area per cortical bone area, pore number per cortical area) were the lowest in the femoral neck (p < 0.05). The maximal osteonal diameter and mean wall width were the highest in the external cortex of the iliac crest (p < 0.05), and the mean cortical width, osteon number per cortical area were the highest in the subtrochanteric femoral shaft (p < 0.05). Some osteonal structural parameters (e.g. min osteonal diameter) were significantly positively correlated (0.29 ≤ R2 ≤ 0.45, p < 0.05) between the external iliac crest and the femoral neck. Conclusions This study reveals heterogeneity in cortical microarchitecture between the external and internal iliac crest cortices, as well as between the iliac crest, the femoral neck and the subtrochanteric femoral shaft. Standard iliac crest biopsy does not reflect accurately cortical microarchitecture of other skeletal sites.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bone histomorphometry, Cortical bone, Femoral neck, Iliac crest, Subtrochanteric femoral shaft
in
Bone Reports
volume
6
pages
8 pages
publisher
Elsevier Inc.
external identifiers
  • scopus:85002373866
DOI
10.1016/j.bonr.2016.11.004
language
English
LU publication?
yes
id
52ec2adb-4dda-4924-840e-430c6deb5a89
date added to LUP
2017-01-25 10:40:50
date last changed
2018-01-07 11:46:40
@article{52ec2adb-4dda-4924-840e-430c6deb5a89,
  abstract     = {<p>Purpose The cortical characteristics of the iliac crest in male have rarely been investigated with quantitative histomorphometry. Also it is still unknown how cortical microarchitecture may vary between the iliac crest and fractures related sites at the proximal femur. We studied the microarchitecture of both external and internal cortices within the iliac crest, and compared the results with femoral neck and subtrochanteric femoral shaft sites. Methods Undecalcified histological sections of the iliac crest were obtained bicortically from cadavers (n = 20, aged 18–82 years, males). They were cut (7 μm) and stained using modified Masson-Goldner stain. Histomorphometric parameters of cortical bone were analysed with low (× 50) and high (× 100) magnification, after identifying cortical bone boundaries using our previously validated method. Within cortical bone area, only complete osteons with typical concentric lamellae and cement line were selected and measured. Results At the iliac crest, the mean cortical width of external cortex was higher than at the internal cortex (p &lt; 0.001). Also, osteon structural parameters, e.g. mean osteonal perimeter, were higher in the external cortex (p &lt; 0.05). In both external and internal cortices, pore number per cortical bone area was higher in young subjects (≤ 50 years) (p &lt; 0.05) while mean pore perimeter was higher in the old subjects (&gt; 50 years) (p &lt; 0.05). Several cortical parameters (e.g. osteon area per cortical bone area, pore number per cortical area) were the lowest in the femoral neck (p &lt; 0.05). The maximal osteonal diameter and mean wall width were the highest in the external cortex of the iliac crest (p &lt; 0.05), and the mean cortical width, osteon number per cortical area were the highest in the subtrochanteric femoral shaft (p &lt; 0.05). Some osteonal structural parameters (e.g. min osteonal diameter) were significantly positively correlated (0.29 ≤ R<sup>2</sup> ≤ 0.45, p &lt; 0.05) between the external iliac crest and the femoral neck. Conclusions This study reveals heterogeneity in cortical microarchitecture between the external and internal iliac crest cortices, as well as between the iliac crest, the femoral neck and the subtrochanteric femoral shaft. Standard iliac crest biopsy does not reflect accurately cortical microarchitecture of other skeletal sites.</p>},
  author       = {Tong, Xiaoyu and Burton, Inari S. and Jurvelin, Jukka S. and Isaksson, Hanna and Kröger, Heikki},
  keyword      = {Bone histomorphometry,Cortical bone,Femoral neck,Iliac crest,Subtrochanteric femoral shaft},
  language     = {eng},
  month        = {06},
  pages        = {9--16},
  publisher    = {Elsevier Inc.},
  series       = {Bone Reports},
  title        = {Iliac crest histomorphometry and skeletal heterogeneity in men},
  url          = {http://dx.doi.org/10.1016/j.bonr.2016.11.004},
  volume       = {6},
  year         = {2017},
}