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Human genes for the alpha and beta chains of complement C4b-binding protein are closely linked in a head-to-tail arrangement

Pardo-Manuel, F ; Rey-Campos, J ; Hillarp, A LU ; Dahlbäck, B LU and Rodriguez de Cordoba, S (1990) In Proceedings of the National Academy of Sciences of the United States of America 87(12). p.32-4529
Abstract

C4b-binding protein (C4BP) is an important component in the regulation of the complement system and also binds the anticoagulant vitamin K-dependent protein S. These activities are performed by distinct, although structurally related, polypeptides of 70 kDa (alpha chain) and 45 kDa (beta chain), respectively. In this report we have investigated the genetic relationships between these polypeptides. Using pulsed field gel electrophoresis analysis we demonstrate that the genes coding for the alpha (C4BP alpha) and beta (C4BP beta) chains are closely linked within the regulator of complement activation gene cluster. In addition, we have determined that the 3' end of the C4BP beta gene lies 3.5-5 kilobases from the 5' end of the C4BP alpha... (More)

C4b-binding protein (C4BP) is an important component in the regulation of the complement system and also binds the anticoagulant vitamin K-dependent protein S. These activities are performed by distinct, although structurally related, polypeptides of 70 kDa (alpha chain) and 45 kDa (beta chain), respectively. In this report we have investigated the genetic relationships between these polypeptides. Using pulsed field gel electrophoresis analysis we demonstrate that the genes coding for the alpha (C4BP alpha) and beta (C4BP beta) chains are closely linked within the regulator of complement activation gene cluster. In addition, we have determined that the 3' end of the C4BP beta gene lies 3.5-5 kilobases from the 5' end of the C4BP alpha gene. These findings support the concept that the C4BP alpha and C4BP beta genes are the result of a gene duplication event.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Carrier Proteins/genetics, Complement Inactivator Proteins, DNA/genetics, DNA Probes, Electrophoresis, Agar Gel, Gene Library, Genes, Genetic Linkage, Glycoproteins, Humans, Macromolecular Substances, Receptors, Complement/genetics, Restriction Mapping
in
Proceedings of the National Academy of Sciences of the United States of America
volume
87
issue
12
pages
32 - 4529
publisher
National Academy of Sciences
external identifiers
  • pmid:2352933
  • scopus:0025371586
ISSN
0027-8424
DOI
10.1073/pnas.87.12.4529
language
English
LU publication?
yes
id
52fc66e5-3c6a-40f8-84b0-84d2d57eb560
date added to LUP
2022-08-29 10:35:47
date last changed
2024-01-03 15:57:13
@article{52fc66e5-3c6a-40f8-84b0-84d2d57eb560,
  abstract     = {{<p>C4b-binding protein (C4BP) is an important component in the regulation of the complement system and also binds the anticoagulant vitamin K-dependent protein S. These activities are performed by distinct, although structurally related, polypeptides of 70 kDa (alpha chain) and 45 kDa (beta chain), respectively. In this report we have investigated the genetic relationships between these polypeptides. Using pulsed field gel electrophoresis analysis we demonstrate that the genes coding for the alpha (C4BP alpha) and beta (C4BP beta) chains are closely linked within the regulator of complement activation gene cluster. In addition, we have determined that the 3' end of the C4BP beta gene lies 3.5-5 kilobases from the 5' end of the C4BP alpha gene. These findings support the concept that the C4BP alpha and C4BP beta genes are the result of a gene duplication event.</p>}},
  author       = {{Pardo-Manuel, F and Rey-Campos, J and Hillarp, A and Dahlbäck, B and Rodriguez de Cordoba, S}},
  issn         = {{0027-8424}},
  keywords     = {{Carrier Proteins/genetics; Complement Inactivator Proteins; DNA/genetics; DNA Probes; Electrophoresis, Agar Gel; Gene Library; Genes; Genetic Linkage; Glycoproteins; Humans; Macromolecular Substances; Receptors, Complement/genetics; Restriction Mapping}},
  language     = {{eng}},
  number       = {{12}},
  pages        = {{32--4529}},
  publisher    = {{National Academy of Sciences}},
  series       = {{Proceedings of the National Academy of Sciences of the United States of America}},
  title        = {{Human genes for the alpha and beta chains of complement C4b-binding protein are closely linked in a head-to-tail arrangement}},
  url          = {{http://dx.doi.org/10.1073/pnas.87.12.4529}},
  doi          = {{10.1073/pnas.87.12.4529}},
  volume       = {{87}},
  year         = {{1990}},
}