Advanced

Main factors controlling microbial community structure and function after reclamation of a tailing pond with aided phytostabilization

Zornoza, R.; Acosta, J. A.; Martinez-Martinez, S.; Faz, A. and Bååth, Erland LU (2015) In Geoderma 245. p.1-10
Abstract
Reclamation on bare tailing ponds has the potential to represent soil genesis in Technosols favoring the understanding of the changes of microbial communities and function. In this study we used phytostabilization aided with calcium carbonate and pig slurry/manure to reclaim an acidic bare tailing pond with the aim of investigating the effect of amending and different species on microbial community structure and function. We sampled after two years of amending and planting: unamended tailing soil (UTS), non-rhizospheric amended tailing soil (ATS), rhizospheric soil from four species, and non-rhizospheric native forest soil (NS), which acted as reference. The application of amendments increased pH up to neutrality, organic carbon (Corg),... (More)
Reclamation on bare tailing ponds has the potential to represent soil genesis in Technosols favoring the understanding of the changes of microbial communities and function. In this study we used phytostabilization aided with calcium carbonate and pig slurry/manure to reclaim an acidic bare tailing pond with the aim of investigating the effect of amending and different species on microbial community structure and function. We sampled after two years of amending and planting: unamended tailing soil (UTS), non-rhizospheric amended tailing soil (ATS), rhizospheric soil from four species, and non-rhizospheric native forest soil (NS), which acted as reference. The application of amendments increased pH up to neutrality, organic carbon (Corg), C/N and aggregate stability, while decreased salinity and heavy metals availability. No effect of rhizosphere was observed on physicochemical properties, metals immobilization and microbial community structure and function. To account for confounding effects due to soil organic matter, microbial properties were expressed per Corg. The high increments in pH and Corg have been the main factors driving changes in microbial community structure and function. Bacterial biomass was higher in UTS, without significant differences among the rest of soils. Fungal biomass followed the trend UTS < ATS = rhizospheric soils <NS. Bacterial growth increased and fungal growth decreased with increasing pH, despite the high availability of metals at low pH. Enzyme activities were lower in UTS, with beta-glucosidase and beta-glucosaminidase activities highly correlated with bacterial growth. Microbial activities were not correlated with the exchangeable fraction of heavy metals, indicating that microbial function is not strongly affected by these metals, likely due to the efficiency of the reclamation procedure to reduce metals toxicity. Changes in microbial community composition were largely explained by changes in pH, heavy metals availability and Corg, with increments in fungal and actinobacterial proportions with soil amending. (C) 2015 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Phospholipid fatty acids, Microbial activity, Amendments, pH, Soil, reclamation
in
Geoderma
volume
245
pages
1 - 10
publisher
Elsevier
external identifiers
  • wos:000350520000001
  • scopus:84921312114
ISSN
0016-7061
DOI
10.1016/j.geoderma.2015.01.007
language
English
LU publication?
yes
id
fa5cc8fd-e13e-499d-8a57-a01908e663e2 (old id 5300347)
date added to LUP
2015-04-27 09:55:18
date last changed
2017-10-01 04:23:37
@article{fa5cc8fd-e13e-499d-8a57-a01908e663e2,
  abstract     = {Reclamation on bare tailing ponds has the potential to represent soil genesis in Technosols favoring the understanding of the changes of microbial communities and function. In this study we used phytostabilization aided with calcium carbonate and pig slurry/manure to reclaim an acidic bare tailing pond with the aim of investigating the effect of amending and different species on microbial community structure and function. We sampled after two years of amending and planting: unamended tailing soil (UTS), non-rhizospheric amended tailing soil (ATS), rhizospheric soil from four species, and non-rhizospheric native forest soil (NS), which acted as reference. The application of amendments increased pH up to neutrality, organic carbon (Corg), C/N and aggregate stability, while decreased salinity and heavy metals availability. No effect of rhizosphere was observed on physicochemical properties, metals immobilization and microbial community structure and function. To account for confounding effects due to soil organic matter, microbial properties were expressed per Corg. The high increments in pH and Corg have been the main factors driving changes in microbial community structure and function. Bacterial biomass was higher in UTS, without significant differences among the rest of soils. Fungal biomass followed the trend UTS &lt; ATS = rhizospheric soils &lt;NS. Bacterial growth increased and fungal growth decreased with increasing pH, despite the high availability of metals at low pH. Enzyme activities were lower in UTS, with beta-glucosidase and beta-glucosaminidase activities highly correlated with bacterial growth. Microbial activities were not correlated with the exchangeable fraction of heavy metals, indicating that microbial function is not strongly affected by these metals, likely due to the efficiency of the reclamation procedure to reduce metals toxicity. Changes in microbial community composition were largely explained by changes in pH, heavy metals availability and Corg, with increments in fungal and actinobacterial proportions with soil amending. (C) 2015 Elsevier B.V. All rights reserved.},
  author       = {Zornoza, R. and Acosta, J. A. and Martinez-Martinez, S. and Faz, A. and Bååth, Erland},
  issn         = {0016-7061},
  keyword      = {Phospholipid fatty acids,Microbial activity,Amendments,pH,Soil,reclamation},
  language     = {eng},
  pages        = {1--10},
  publisher    = {Elsevier},
  series       = {Geoderma},
  title        = {Main factors controlling microbial community structure and function after reclamation of a tailing pond with aided phytostabilization},
  url          = {http://dx.doi.org/10.1016/j.geoderma.2015.01.007},
  volume       = {245},
  year         = {2015},
}