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Nonfouling Tunable beta CD Dextran Polymer Films for Protein Applications

Stade, Lars W.; Nielsen, Thorbjorn T.; Duroux, Laurent; Hinge, Mogens; Shimizu, Kyoko; Gurevich, Leonid; Kristensen, Peter K.; Wingren, Christer LU and Larsen, Kim L. (2015) In ACS Applied Materials and Interfaces 7(7). p.4160-4168
Abstract
Polymeric beta-cyclodextrin (beta CD) films tunable with respect to thickness and beta CD content were prepared in order to develop a suitable platform, allowing for inclusion of nonpolar guest molecules in the beta CD cavity, while suppressing nonspecific protein adsorption. The beta CD films were synthesized from linear beta CD dextran polymers, and grafted onto silicon oxide surfaces by "click" chemistry. Topographic and morphological characteristics are controllable by reaction conditions and polymer type, with average film heights from 2.5 to 12.5 nm. Reversible introduction of electrostatic charges in the beta CD dextran by complex formation with 1-adamantanecarboxylic acid prior to surface grafting resulted in a thinner and denser... (More)
Polymeric beta-cyclodextrin (beta CD) films tunable with respect to thickness and beta CD content were prepared in order to develop a suitable platform, allowing for inclusion of nonpolar guest molecules in the beta CD cavity, while suppressing nonspecific protein adsorption. The beta CD films were synthesized from linear beta CD dextran polymers, and grafted onto silicon oxide surfaces by "click" chemistry. Topographic and morphological characteristics are controllable by reaction conditions and polymer type, with average film heights from 2.5 to 12.5 nm. Reversible introduction of electrostatic charges in the beta CD dextran by complex formation with 1-adamantanecarboxylic acid prior to surface grafting resulted in a thinner and denser film, presumably by decompaction of the polymers. Total internal reflection fluorescence spectroscopy (TIRF) was employed to evaluate the accessibility of beta CD cavities to the fluorescent probe 2-anilinonaphthalene-6-sulfonic acid. Only a minor fraction of the beta CD cavities was accessible in the thicker and less dense films; however, accessibility was largely improved with increased ionic strength using NaCl up to 1 M. Antifouling properties of the beta CD dextran polymer films were assessed by TIRF real-time monitoring, using bovine serum albumin as a model protein, and showed a 5- to 10-fold reduction in nonspecific adsorption as compared to a bare quartz surface with the degree of reduction reflecting film thickness and interfacial polymer density. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
beta-cyclodextrin, dextran, polymer films, protein immobilization, nonspecific adsorption, total internal reflection fluorescence, spectroscopy
in
ACS Applied Materials and Interfaces
volume
7
issue
7
pages
4160 - 4168
publisher
The American Chemical Society
external identifiers
  • wos:000350193000035
  • scopus:84923763018
ISSN
1944-8244
DOI
10.1021/am508350r
language
English
LU publication?
yes
id
e2f35517-ec3d-47a8-b9f4-9f864182f417 (old id 5300499)
date added to LUP
2015-04-27 10:21:33
date last changed
2017-09-03 03:28:16
@article{e2f35517-ec3d-47a8-b9f4-9f864182f417,
  abstract     = {Polymeric beta-cyclodextrin (beta CD) films tunable with respect to thickness and beta CD content were prepared in order to develop a suitable platform, allowing for inclusion of nonpolar guest molecules in the beta CD cavity, while suppressing nonspecific protein adsorption. The beta CD films were synthesized from linear beta CD dextran polymers, and grafted onto silicon oxide surfaces by "click" chemistry. Topographic and morphological characteristics are controllable by reaction conditions and polymer type, with average film heights from 2.5 to 12.5 nm. Reversible introduction of electrostatic charges in the beta CD dextran by complex formation with 1-adamantanecarboxylic acid prior to surface grafting resulted in a thinner and denser film, presumably by decompaction of the polymers. Total internal reflection fluorescence spectroscopy (TIRF) was employed to evaluate the accessibility of beta CD cavities to the fluorescent probe 2-anilinonaphthalene-6-sulfonic acid. Only a minor fraction of the beta CD cavities was accessible in the thicker and less dense films; however, accessibility was largely improved with increased ionic strength using NaCl up to 1 M. Antifouling properties of the beta CD dextran polymer films were assessed by TIRF real-time monitoring, using bovine serum albumin as a model protein, and showed a 5- to 10-fold reduction in nonspecific adsorption as compared to a bare quartz surface with the degree of reduction reflecting film thickness and interfacial polymer density.},
  author       = {Stade, Lars W. and Nielsen, Thorbjorn T. and Duroux, Laurent and Hinge, Mogens and Shimizu, Kyoko and Gurevich, Leonid and Kristensen, Peter K. and Wingren, Christer and Larsen, Kim L.},
  issn         = {1944-8244},
  keyword      = {beta-cyclodextrin,dextran,polymer films,protein immobilization,nonspecific adsorption,total internal reflection fluorescence,spectroscopy},
  language     = {eng},
  number       = {7},
  pages        = {4160--4168},
  publisher    = {The American Chemical Society},
  series       = {ACS Applied Materials and Interfaces},
  title        = {Nonfouling Tunable beta CD Dextran Polymer Films for Protein Applications},
  url          = {http://dx.doi.org/10.1021/am508350r},
  volume       = {7},
  year         = {2015},
}