Effect of carbon dioxide and temperature on H2 producing cultures of the extreme thermophile, Caldicellulosiruptor saccharolyticus
(2004) 15th World Hydrogen Energy Conference- Abstract
- Carbon dioxide could replace nitrogen gas as stripping gas for the production of H2 by the extreme thermophile, Caldicellulosiruptor saccharolyticus, provided that the pH is kept below 7.0. Above this pH, ≥77 mM sodium bicarbonate will be introduced as an extra salt burden to the culture. High concentrations of sugars and salts contribute each to the inhibition of growth and H2 production, which is most probably due to the osmolality. As a consequence, using CO2 as stripping gas might lower the maximum allowable acetate concentration in culture, and therefore the productivity of the culture at neutral pH. Although the growth rate was not affected by CO2, lower biomass and hydrogen yields were obtained. This could be due to an increased... (More)
- Carbon dioxide could replace nitrogen gas as stripping gas for the production of H2 by the extreme thermophile, Caldicellulosiruptor saccharolyticus, provided that the pH is kept below 7.0. Above this pH, ≥77 mM sodium bicarbonate will be introduced as an extra salt burden to the culture. High concentrations of sugars and salts contribute each to the inhibition of growth and H2 production, which is most probably due to the osmolality. As a consequence, using CO2 as stripping gas might lower the maximum allowable acetate concentration in culture, and therefore the productivity of the culture at neutral pH. Although the growth rate was not affected by CO2, lower biomass and hydrogen yields were obtained. This could be due to an increased lactate production. In a thermodynamic study an estimation of the activation enthalpies was made for both hydrogen production and its thermal inactivation, being 45.9 kJ/mol and 112.3 kJ/mol, respectively. Hydrogen production showed a wider temperature range (estimated 40 - 90° C) than growth (49.6 – 85.7° C). (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/530649
- author
- van Niel, Ed LU ; Martens, A.A. and Claassen, P.A.M.
- organization
- publishing date
- 2004
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Caldicellulosiruptor saccharolyticus, osmolality, activation enthalpy, carbon dioxide, extreme thermophile
- host publication
- Proceedings of the 15th World Hydrogen Energy Conference
- pages
- 10 pages
- conference name
- 15th World Hydrogen Energy Conference
- conference location
- Yokohama, Japan
- conference dates
- 2004-06-30
- language
- English
- LU publication?
- yes
- additional info
- Published as a CD.
- id
- f04a781d-f6a3-4244-b5fe-eeb0d155bedd (old id 530649)
- date added to LUP
- 2016-04-04 13:50:28
- date last changed
- 2018-11-21 21:16:39
@inproceedings{f04a781d-f6a3-4244-b5fe-eeb0d155bedd, abstract = {{Carbon dioxide could replace nitrogen gas as stripping gas for the production of H2 by the extreme thermophile, Caldicellulosiruptor saccharolyticus, provided that the pH is kept below 7.0. Above this pH, ≥77 mM sodium bicarbonate will be introduced as an extra salt burden to the culture. High concentrations of sugars and salts contribute each to the inhibition of growth and H2 production, which is most probably due to the osmolality. As a consequence, using CO2 as stripping gas might lower the maximum allowable acetate concentration in culture, and therefore the productivity of the culture at neutral pH. Although the growth rate was not affected by CO2, lower biomass and hydrogen yields were obtained. This could be due to an increased lactate production. In a thermodynamic study an estimation of the activation enthalpies was made for both hydrogen production and its thermal inactivation, being 45.9 kJ/mol and 112.3 kJ/mol, respectively. Hydrogen production showed a wider temperature range (estimated 40 - 90° C) than growth (49.6 – 85.7° C).}}, author = {{van Niel, Ed and Martens, A.A. and Claassen, P.A.M.}}, booktitle = {{Proceedings of the 15th World Hydrogen Energy Conference}}, keywords = {{Caldicellulosiruptor saccharolyticus; osmolality; activation enthalpy; carbon dioxide; extreme thermophile}}, language = {{eng}}, title = {{Effect of carbon dioxide and temperature on H2 producing cultures of the extreme thermophile, Caldicellulosiruptor saccharolyticus}}, url = {{https://lup.lub.lu.se/search/files/6217751/931378.pdf}}, year = {{2004}}, }