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Fabrication of user-friendly and biomimetic 1,1′-carbonyldiimidazole cross-linked gelatin/agar microfluidic devices

Jocic, Simonne; Mestres, Gemma and Tenje, Maria LU (2017) In Materials Science and Engineering C 76. p.1175-1180
Abstract

We have developed a straightforward technique for fabricating user-friendly and biomimetic microfluidic devices out of a gelatin/agar gel cross-linked with 1,1′-carbonyldiimidazole. The fabrication procedure requires only inexpensive starting materials such as glass capillaries and wires to mold 3D cylindrical channels into the gel with the possibility of achieving channel diameters of 375 μm and 1000 μm. We demonstrate that the channel absent of gel injury can retain fluid within its dimensions for at least 7 h. We also show that the device material does not autofluoresce nor provide hindrances with fluorescent imaging. A discussion of the chemical linkage identities of cross-linked gelatin/agar is included via ATR-FTIR studies.... (More)

We have developed a straightforward technique for fabricating user-friendly and biomimetic microfluidic devices out of a gelatin/agar gel cross-linked with 1,1′-carbonyldiimidazole. The fabrication procedure requires only inexpensive starting materials such as glass capillaries and wires to mold 3D cylindrical channels into the gel with the possibility of achieving channel diameters of 375 μm and 1000 μm. We demonstrate that the channel absent of gel injury can retain fluid within its dimensions for at least 7 h. We also show that the device material does not autofluoresce nor provide hindrances with fluorescent imaging. A discussion of the chemical linkage identities of cross-linked gelatin/agar is included via ATR-FTIR studies. Crosslinking of the gelatin/agar is further confirmed by the lack of a gel to sol transition at physiological temperature as assessed by DSC measurements. SEM micrographs that demonstrate the 100 nm mean pore width of the cross-linked gelatin/agar are provided. This device is considered biomimetic because it represents components present in the natural extracellular matrix such as collagen and proteoglycans in the form of cross-linked gelatin/agar.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
1,1′-carbonyldiimidazole, Agar, ATR-FTIR, Biomicrofluidics, Gelatin
in
Materials Science and Engineering C
volume
76
pages
6 pages
publisher
Elsevier
external identifiers
  • scopus:85016415924
  • wos:000405541600134
ISSN
0928-4931
DOI
10.1016/j.msec.2017.03.030
language
English
LU publication?
yes
id
531a03df-3784-4407-9aaa-b2e016b7b70e
date added to LUP
2017-04-11 08:35:23
date last changed
2018-01-07 11:58:49
@article{531a03df-3784-4407-9aaa-b2e016b7b70e,
  abstract     = {<p>We have developed a straightforward technique for fabricating user-friendly and biomimetic microfluidic devices out of a gelatin/agar gel cross-linked with 1,1′-carbonyldiimidazole. The fabrication procedure requires only inexpensive starting materials such as glass capillaries and wires to mold 3D cylindrical channels into the gel with the possibility of achieving channel diameters of 375 μm and 1000 μm. We demonstrate that the channel absent of gel injury can retain fluid within its dimensions for at least 7 h. We also show that the device material does not autofluoresce nor provide hindrances with fluorescent imaging. A discussion of the chemical linkage identities of cross-linked gelatin/agar is included via ATR-FTIR studies. Crosslinking of the gelatin/agar is further confirmed by the lack of a gel to sol transition at physiological temperature as assessed by DSC measurements. SEM micrographs that demonstrate the 100 nm mean pore width of the cross-linked gelatin/agar are provided. This device is considered biomimetic because it represents components present in the natural extracellular matrix such as collagen and proteoglycans in the form of cross-linked gelatin/agar.</p>},
  author       = {Jocic, Simonne and Mestres, Gemma and Tenje, Maria},
  issn         = {0928-4931},
  keyword      = {1,1′-carbonyldiimidazole,Agar,ATR-FTIR,Biomicrofluidics,Gelatin},
  language     = {eng},
  month        = {07},
  pages        = {1175--1180},
  publisher    = {Elsevier},
  series       = {Materials Science and Engineering C},
  title        = {Fabrication of user-friendly and biomimetic 1,1′-carbonyldiimidazole cross-linked gelatin/agar microfluidic devices},
  url          = {http://dx.doi.org/10.1016/j.msec.2017.03.030},
  volume       = {76},
  year         = {2017},
}