W41/W41 blastocyst complementation: a system for genetic modeling of hematopoiesis.
(2010) In Blood 115(1). p.47-50- Abstract
- We report a rapid and highly efficient approach to generate mice in which the hematopoietic system is derived from embryonic stem (ES) cells. We show that ES cells injected into blastocysts from the c-kit deficient W41/W41 mouse strain have a clear advantage over the W41/W41 blastocyst-derived inner cell mass cells in founding the hematopoietic system. Fetal liver HSCs from W41/W41 blastocyst complementation embryos can be transplanted to establish large cohorts of bone marrow chimeras with hematopoiesis of practically pure ES cell origin. Using ES cells with site-directed modifications we show how this system can be employed to drive inducible transgene expression in hematopoietic cells in a robust and reliable manner both in vitro and in... (More)
- We report a rapid and highly efficient approach to generate mice in which the hematopoietic system is derived from embryonic stem (ES) cells. We show that ES cells injected into blastocysts from the c-kit deficient W41/W41 mouse strain have a clear advantage over the W41/W41 blastocyst-derived inner cell mass cells in founding the hematopoietic system. Fetal liver HSCs from W41/W41 blastocyst complementation embryos can be transplanted to establish large cohorts of bone marrow chimeras with hematopoiesis of practically pure ES cell origin. Using ES cells with site-directed modifications we show how this system can be employed to drive inducible transgene expression in hematopoietic cells in a robust and reliable manner both in vitro and in vivo. The approach avoids the cost and time constraints associated with the creation of standard transgenic mouse strains while taking advantage of the sophisticated site-directed manipulations that are possible in ES cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1499893
- author
- Jansson, Lina LU and Larsson, Jonas LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Blood
- volume
- 115
- issue
- 1
- pages
- 47 - 50
- publisher
- American Society of Hematology
- external identifiers
-
- wos:000273389600011
- pmid:19864644
- scopus:74949125790
- pmid:19864644
- ISSN
- 1528-0020
- DOI
- 10.1182/blood-2009-07-235622
- language
- English
- LU publication?
- yes
- id
- 531df94f-354d-46df-a190-7c00d991a676 (old id 1499893)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/19864644?dopt=Abstract
- date added to LUP
- 2016-04-01 10:28:23
- date last changed
- 2025-04-04 15:25:02
@article{531df94f-354d-46df-a190-7c00d991a676,
abstract = {{We report a rapid and highly efficient approach to generate mice in which the hematopoietic system is derived from embryonic stem (ES) cells. We show that ES cells injected into blastocysts from the c-kit deficient W41/W41 mouse strain have a clear advantage over the W41/W41 blastocyst-derived inner cell mass cells in founding the hematopoietic system. Fetal liver HSCs from W41/W41 blastocyst complementation embryos can be transplanted to establish large cohorts of bone marrow chimeras with hematopoiesis of practically pure ES cell origin. Using ES cells with site-directed modifications we show how this system can be employed to drive inducible transgene expression in hematopoietic cells in a robust and reliable manner both in vitro and in vivo. The approach avoids the cost and time constraints associated with the creation of standard transgenic mouse strains while taking advantage of the sophisticated site-directed manipulations that are possible in ES cells.}},
author = {{Jansson, Lina and Larsson, Jonas}},
issn = {{1528-0020}},
language = {{eng}},
number = {{1}},
pages = {{47--50}},
publisher = {{American Society of Hematology}},
series = {{Blood}},
title = {{W41/W41 blastocyst complementation: a system for genetic modeling of hematopoiesis.}},
url = {{http://dx.doi.org/10.1182/blood-2009-07-235622}},
doi = {{10.1182/blood-2009-07-235622}},
volume = {{115}},
year = {{2010}},
}