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Genome-wide gene expression profiling of low-dose, long-term exposure of human osteosarcoma cells to bisphenol A and its analogs bisphenols AF and S.

Fic, A ; Jurković Mlakar, S ; Juvan, P ; Mlakar, V ; Marc, J ; Sollner Dolenc, M ; Broberg Palmgren, Karin LU orcid and Peterlin Mašič, L (2015) In Toxicology in Vitro 29(5). p.1060-1069
Abstract
The bisphenols AF (BPAF) and S (BPS) are structural analogs of the endocrine disruptor bisphenol A (BPA), and are used in common products as a replacement for BPA. To elucidate genome-wide gene expression responses, estrogen-dependent osteosarcoma cells were cultured with 10nM BPA, BPAF, or BPS, for 8h and 3months. Genome-wide gene expression was analyzed using the Illumina Expression BeadChip. Three months exposure had significant effects on gene expression, particularly for BPS, followed by BPAF and BPA, according to the number of differentially expressed genes (1980, 778, 60, respectively), the magnitude of changes in gene expression, and the number of enriched biological processes (800, 415, 33, respectively) and pathways (77, 52, 6,... (More)
The bisphenols AF (BPAF) and S (BPS) are structural analogs of the endocrine disruptor bisphenol A (BPA), and are used in common products as a replacement for BPA. To elucidate genome-wide gene expression responses, estrogen-dependent osteosarcoma cells were cultured with 10nM BPA, BPAF, or BPS, for 8h and 3months. Genome-wide gene expression was analyzed using the Illumina Expression BeadChip. Three months exposure had significant effects on gene expression, particularly for BPS, followed by BPAF and BPA, according to the number of differentially expressed genes (1980, 778, 60, respectively), the magnitude of changes in gene expression, and the number of enriched biological processes (800, 415, 33, respectively) and pathways (77, 52, 6, respectively). 'Embryonic skeletal system development' was the most enriched bone-related process, which was affected only by BPAF and BPS. Interestingly, all three bisphenols showed highest down-regulation of genes related to the cardiovascular system (e.g., NPPB, NPR3, TXNIP). BPA only and BPA/BPAF/BPS also affected genes related to the immune system and fetal development, respectively. For BPAF and BPS, the 'isoprenoid biosynthetic process' was enriched (up-regulated genes: HMGCS1, PDSS1, ACAT2, RCE1, DHDDS). Compared to BPA, BPAF and BPS had more effects on gene expression after long-term exposure. These findings stress the need for careful toxicological characterization of BPA analogs in the future. (Less)
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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Toxicology in Vitro
volume
29
issue
5
pages
1060 - 1069
publisher
Elsevier
external identifiers
  • pmid:25912373
  • wos:000356981500028
  • scopus:84929459075
  • pmid:25912373
ISSN
1879-3177
DOI
10.1016/j.tiv.2015.03.014
language
English
LU publication?
yes
id
9da468fc-6c0b-4d00-bfe7-217deeca11b7 (old id 5337844)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25912373?dopt=Abstract
date added to LUP
2016-04-01 10:14:35
date last changed
2022-01-25 21:16:40
@article{9da468fc-6c0b-4d00-bfe7-217deeca11b7,
  abstract     = {{The bisphenols AF (BPAF) and S (BPS) are structural analogs of the endocrine disruptor bisphenol A (BPA), and are used in common products as a replacement for BPA. To elucidate genome-wide gene expression responses, estrogen-dependent osteosarcoma cells were cultured with 10nM BPA, BPAF, or BPS, for 8h and 3months. Genome-wide gene expression was analyzed using the Illumina Expression BeadChip. Three months exposure had significant effects on gene expression, particularly for BPS, followed by BPAF and BPA, according to the number of differentially expressed genes (1980, 778, 60, respectively), the magnitude of changes in gene expression, and the number of enriched biological processes (800, 415, 33, respectively) and pathways (77, 52, 6, respectively). 'Embryonic skeletal system development' was the most enriched bone-related process, which was affected only by BPAF and BPS. Interestingly, all three bisphenols showed highest down-regulation of genes related to the cardiovascular system (e.g., NPPB, NPR3, TXNIP). BPA only and BPA/BPAF/BPS also affected genes related to the immune system and fetal development, respectively. For BPAF and BPS, the 'isoprenoid biosynthetic process' was enriched (up-regulated genes: HMGCS1, PDSS1, ACAT2, RCE1, DHDDS). Compared to BPA, BPAF and BPS had more effects on gene expression after long-term exposure. These findings stress the need for careful toxicological characterization of BPA analogs in the future.}},
  author       = {{Fic, A and Jurković Mlakar, S and Juvan, P and Mlakar, V and Marc, J and Sollner Dolenc, M and Broberg Palmgren, Karin and Peterlin Mašič, L}},
  issn         = {{1879-3177}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{1060--1069}},
  publisher    = {{Elsevier}},
  series       = {{Toxicology in Vitro}},
  title        = {{Genome-wide gene expression profiling of low-dose, long-term exposure of human osteosarcoma cells to bisphenol A and its analogs bisphenols AF and S.}},
  url          = {{http://dx.doi.org/10.1016/j.tiv.2015.03.014}},
  doi          = {{10.1016/j.tiv.2015.03.014}},
  volume       = {{29}},
  year         = {{2015}},
}