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Removal of endotoxin in the purification of histone H1. 5 from Escherichia coli

Pyo, Sang-Hyun LU ; Park, Heung-Bok ; Hong, Seung-Suh and Kim, Jin-Hyun (2001) In Biotechnology Letters 23(9). p.737-740
Abstract
Triton X-114 and cation-exchange chromatography, SP-Sepharose FF, removed endotoxins from solutions containing recombinant histone H1.5. Dissociated endotoxins were removed but fractions containing histone H1.5 were enhanced in the elution step. The final concentration of endotoxins, measured by a limulus amoebocyte lysate (LAL) assay, was below 0.05 EU mg−1 histone H1.5. The recovery of protein was above 95%.
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author
; ; and
publishing date
type
Contribution to journal
publication status
published
in
Biotechnology Letters
volume
23
issue
9
pages
4 pages
publisher
Springer Science and Business Media B.V.
ISSN
0141-5492
DOI
10.1023/A:1010307120512
language
English
LU publication?
no
id
5340be03-f8fe-479c-a30b-d5a828569787
date added to LUP
2025-09-05 17:41:53
date last changed
2025-09-17 12:17:25
@article{5340be03-f8fe-479c-a30b-d5a828569787,
  abstract     = {{Triton X-114 and cation-exchange chromatography, SP-Sepharose FF, removed endotoxins from solutions containing recombinant histone H1.5. Dissociated endotoxins were removed but fractions containing histone H1.5 were enhanced in the elution step. The final concentration of endotoxins, measured by a limulus amoebocyte lysate (LAL) assay, was below 0.05 EU mg−1 histone H1.5. The recovery of protein was above 95%.}},
  author       = {{Pyo, Sang-Hyun and Park, Heung-Bok and Hong, Seung-Suh and Kim, Jin-Hyun}},
  issn         = {{0141-5492}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{737--740}},
  publisher    = {{Springer Science and Business Media B.V.}},
  series       = {{Biotechnology Letters}},
  title        = {{Removal of endotoxin in the purification of histone H1. 5 from Escherichia coli}},
  url          = {{http://dx.doi.org/10.1023/A:1010307120512}},
  doi          = {{10.1023/A:1010307120512}},
  volume       = {{23}},
  year         = {{2001}},
}