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Characterising Bone Material Composition and Structure in the Ovariectomized (OVX) Rat Model of Osteoporosis.

Mathavan, Neashan LU ; Turunen, Mikael J; Tägil, Magnus LU and Isaksson, Hanna LU (2015) In Calcified Tissue International 97(2). p.134-144
Abstract
The ovariectomized (OVX) rat model is well established in investigations of osteoporosis and osteoporotic therapies. Advent of techniques such as Fourier-transform infrared (FTIR) spectroscopy and small angle X-ray scattering (SAXS) facilitate characterization of bone composition and mineral structure, respectively, which are key determinants of bone strength. Limited publications exist on the implementation of these techniques in the OVX rat model. At 12 weeks of age, female Sprague-Dawley rats were either sham-operated (n = 6) or ovariectomized (n = 6) and sacrificed 18 weeks later. L2 lumbar vertebrae and proximal tibiae were assessed by µCT, FTIR and SAXS. Presence of extensive trabecular deterioration in the µCT data confirmed the... (More)
The ovariectomized (OVX) rat model is well established in investigations of osteoporosis and osteoporotic therapies. Advent of techniques such as Fourier-transform infrared (FTIR) spectroscopy and small angle X-ray scattering (SAXS) facilitate characterization of bone composition and mineral structure, respectively, which are key determinants of bone strength. Limited publications exist on the implementation of these techniques in the OVX rat model. At 12 weeks of age, female Sprague-Dawley rats were either sham-operated (n = 6) or ovariectomized (n = 6) and sacrificed 18 weeks later. L2 lumbar vertebrae and proximal tibiae were assessed by µCT, FTIR and SAXS. Presence of extensive trabecular deterioration in the µCT data confirmed the onset of osteoporosis. FTIR compositional parameters were determined including measures of degree of mineralization, crystallinity, collagen maturity and acid phosphate content. Mineral crystal thickness was determined from the SAXS data using two approaches available in literature. Compositionally, a decline in the heterogeneity of acid phosphate content was observed while measures of crystallinity and collagen maturity remained unaltered. Using an iterative curve fitting method, OVX-induced increases in the mineral crystal thickness of 3.8 and 7.8 % (p < 0.05) were noted in the trabecular of the vertebra and tibia, respectively. In conclusion, implementation of FTIR and SAXS techniques in the OVX rat model, identified no significant compositional changes while substantiating thickening of the mineral crystals as a general structural feature of OVX-induced osteoporosis in rats. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bone structure, Osteoporosis, Bone composition, Fourier-transform infrared spectroscopy FTIR, Small angle X-ray scattering SAXS
in
Calcified Tissue International
volume
97
issue
2
pages
134 - 144
publisher
Springer
external identifiers
  • pmid:25894067
  • wos:000357460200005
  • scopus:84943354090
ISSN
1432-0827
DOI
10.1007/s00223-015-9991-7
language
English
LU publication?
yes
id
6a02d71b-ee03-4d15-9d27-9f6982ecce69 (old id 5341211)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25894067?dopt=Abstract
date added to LUP
2015-05-04 17:31:21
date last changed
2017-10-29 03:21:27
@article{6a02d71b-ee03-4d15-9d27-9f6982ecce69,
  abstract     = {The ovariectomized (OVX) rat model is well established in investigations of osteoporosis and osteoporotic therapies. Advent of techniques such as Fourier-transform infrared (FTIR) spectroscopy and small angle X-ray scattering (SAXS) facilitate characterization of bone composition and mineral structure, respectively, which are key determinants of bone strength. Limited publications exist on the implementation of these techniques in the OVX rat model. At 12 weeks of age, female Sprague-Dawley rats were either sham-operated (n = 6) or ovariectomized (n = 6) and sacrificed 18 weeks later. L2 lumbar vertebrae and proximal tibiae were assessed by µCT, FTIR and SAXS. Presence of extensive trabecular deterioration in the µCT data confirmed the onset of osteoporosis. FTIR compositional parameters were determined including measures of degree of mineralization, crystallinity, collagen maturity and acid phosphate content. Mineral crystal thickness was determined from the SAXS data using two approaches available in literature. Compositionally, a decline in the heterogeneity of acid phosphate content was observed while measures of crystallinity and collagen maturity remained unaltered. Using an iterative curve fitting method, OVX-induced increases in the mineral crystal thickness of 3.8 and 7.8 % (p &lt; 0.05) were noted in the trabecular of the vertebra and tibia, respectively. In conclusion, implementation of FTIR and SAXS techniques in the OVX rat model, identified no significant compositional changes while substantiating thickening of the mineral crystals as a general structural feature of OVX-induced osteoporosis in rats.},
  author       = {Mathavan, Neashan and Turunen, Mikael J and Tägil, Magnus and Isaksson, Hanna},
  issn         = {1432-0827},
  keyword      = {Bone structure,Osteoporosis,Bone composition,Fourier-transform infrared spectroscopy FTIR,Small angle X-ray scattering SAXS},
  language     = {eng},
  number       = {2},
  pages        = {134--144},
  publisher    = {Springer},
  series       = {Calcified Tissue International},
  title        = {Characterising Bone Material Composition and Structure in the Ovariectomized (OVX) Rat Model of Osteoporosis.},
  url          = {http://dx.doi.org/10.1007/s00223-015-9991-7},
  volume       = {97},
  year         = {2015},
}