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Engineering of Saccharomyces cerevisiae for the production of poly-3-d-hydroxybutyrate from xylose.

Sandström, Anders LU ; Muñoz de las Heras, Alejandro LU ; Nunes, Diogo JP LU and Gorwa-Grauslund, Marie-Francoise LU (2015) In AMB Express 5.
Abstract
Poly-3-d-hydroxybutyrate (PHB) is a promising biopolymer naturally produced by several bacterial species. In the present study, the robust baker's yeast Saccharomyces cerevisiae was engineered to produce PHB from xylose, the main pentose found in lignocellulosic biomass. The PHB pathway genes from the well-characterized PHB producer Cupriavidus necator were introduced in recombinant S. cerevisiae strains already capable of pentose utilization by introduction of the fungal genes for xylose utilization from the yeast Scheffersomyces stipitis. PHB production from xylose was successfully demonstrated in shake-flasks experiments, with PHB yield of 1.17 ± 0.18 mg PHB g(-1) xylose. Under well-controlled fully aerobic conditions, a titer of 101.7... (More)
Poly-3-d-hydroxybutyrate (PHB) is a promising biopolymer naturally produced by several bacterial species. In the present study, the robust baker's yeast Saccharomyces cerevisiae was engineered to produce PHB from xylose, the main pentose found in lignocellulosic biomass. The PHB pathway genes from the well-characterized PHB producer Cupriavidus necator were introduced in recombinant S. cerevisiae strains already capable of pentose utilization by introduction of the fungal genes for xylose utilization from the yeast Scheffersomyces stipitis. PHB production from xylose was successfully demonstrated in shake-flasks experiments, with PHB yield of 1.17 ± 0.18 mg PHB g(-1) xylose. Under well-controlled fully aerobic conditions, a titer of 101.7 mg PHB L(-1) was reached within 48 hours, with a PHB yield of 1.99 ± 0.15 mg PHB g(-1) xylose, thereby demonstrating the potential of this host for PHB production from lignocellulose. (Less)
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Contribution to journal
publication status
published
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in
AMB Express
volume
5
publisher
Springer Open
external identifiers
  • pmid:25852991
  • wos:000358074100001
  • scopus:84923852157
ISSN
2191-0855
DOI
10.1186/s13568-015-0100-0
language
English
LU publication?
yes
id
02b9a40d-05f5-48bf-b81b-eb5a116b0b02 (old id 5345164)
date added to LUP
2015-04-30 10:22:51
date last changed
2017-08-27 04:50:26
@article{02b9a40d-05f5-48bf-b81b-eb5a116b0b02,
  abstract     = {Poly-3-d-hydroxybutyrate (PHB) is a promising biopolymer naturally produced by several bacterial species. In the present study, the robust baker's yeast Saccharomyces cerevisiae was engineered to produce PHB from xylose, the main pentose found in lignocellulosic biomass. The PHB pathway genes from the well-characterized PHB producer Cupriavidus necator were introduced in recombinant S. cerevisiae strains already capable of pentose utilization by introduction of the fungal genes for xylose utilization from the yeast Scheffersomyces stipitis. PHB production from xylose was successfully demonstrated in shake-flasks experiments, with PHB yield of 1.17 ± 0.18 mg PHB g(-1) xylose. Under well-controlled fully aerobic conditions, a titer of 101.7 mg PHB L(-1) was reached within 48 hours, with a PHB yield of 1.99 ± 0.15 mg PHB g(-1) xylose, thereby demonstrating the potential of this host for PHB production from lignocellulose.},
  articleno    = {14},
  author       = {Sandström, Anders and Muñoz de las Heras, Alejandro and Nunes, Diogo JP and Gorwa-Grauslund, Marie-Francoise},
  issn         = {2191-0855},
  language     = {eng},
  publisher    = {Springer Open},
  series       = {AMB Express},
  title        = {Engineering of Saccharomyces cerevisiae for the production of poly-3-d-hydroxybutyrate from xylose.},
  url          = {http://dx.doi.org/10.1186/s13568-015-0100-0},
  volume       = {5},
  year         = {2015},
}