Simultaneous registration of intraabdominal and intravesical pressures during cystometry in conscious rats-Effects of bladder outlet obstruction and intravesical PGE(2).
Lee, Tack LU ; Andersson, Karl-Erik LU
; Streng, Tomi
LU
and Hedlund, Petter
LU
(2008)
In Neurourology and Urodynamics
27(1).
p.88-95
- Abstract
- AIMS: A method was developed and evaluated to simultaneously register intraabdominal pressure (IAP) and intravesical pressure (IVP) during cystometry in conscious rats. In addition, IAP and IVP were recorded in rats with experimental detrusor overactivity (DO). METHODS: Sprague Dawley rats (n = 24) were used. Six female rats were subjected to partial bladder outlet obstruction for 2 weeks. A catheter was implanted into the bladder to record the IVP, and a balloon-fitted catheter was positioned in the abdominal cavity to record the IAP. PGE(2) was given intravesically to induce DO. Detrusor pressure (DP) was defined as the IVP corrected for IAP. RESULTS: Recorded as increases in IAP, all rats of both sexes exhibited abdominal straining... (More)
- AIMS: A method was developed and evaluated to simultaneously register intraabdominal pressure (IAP) and intravesical pressure (IVP) during cystometry in conscious rats. In addition, IAP and IVP were recorded in rats with experimental detrusor overactivity (DO). METHODS: Sprague Dawley rats (n = 24) were used. Six female rats were subjected to partial bladder outlet obstruction for 2 weeks. A catheter was implanted into the bladder to record the IVP, and a balloon-fitted catheter was positioned in the abdominal cavity to record the IAP. PGE(2) was given intravesically to induce DO. Detrusor pressure (DP) was defined as the IVP corrected for IAP. RESULTS: Recorded as increases in IAP, all rats of both sexes exhibited abdominal straining during every void. In controls, a maximal IAP of 6.0 +/- 1.4 cmH(2)O (range 3-15 cmH(2)O) was registered (n = 12) at the time of the flow pressure (FP). Intravesical administration of PGE(2) or BOO did not affect the IAP at basal pressure, FP or micturition pressure. Changes in IAP due to movement or non-voiding-related straining were subtracted from IVP to generate DP and to visualize DO after BOO or intravesical PGE(2). CONCLUSIONS: The conscious rat uses abdominal straining during voiding, and maximal IAP is recorded at the onset of urinary flow. Simultaneous registration of IAP and IVP during the micturition cycle in conscious rats is a convenient method for accurate quantification of pressures inside the bladder and for studying "true" DO without interference from movement artifacts. Copyright 2007 Wiley-Liss, Inc (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/539823
- author
- Lee, Tack
LU
; Andersson, Karl-Erik
LU
; Streng, Tomi
LU
and Hedlund, Petter
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Neurourology and Urodynamics
- volume
- 27
- issue
- 1
- pages
- 88 - 95
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000252241100016
- scopus:37849045528
- pmid:17565725
- ISSN
- 0733-2467
- DOI
- 10.1002/nau.20460
- language
- English
- LU publication?
- yes
- id
- 3b4a7e26-e099-40ed-adb7-36929cb03221 (old id 539823)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17565725&dopt=Abstract
- date added to LUP
- 2016-04-01 12:09:54
- date last changed
- 2022-01-26 23:41:57
@article{3b4a7e26-e099-40ed-adb7-36929cb03221,
abstract = {{AIMS: A method was developed and evaluated to simultaneously register intraabdominal pressure (IAP) and intravesical pressure (IVP) during cystometry in conscious rats. In addition, IAP and IVP were recorded in rats with experimental detrusor overactivity (DO). METHODS: Sprague Dawley rats (n = 24) were used. Six female rats were subjected to partial bladder outlet obstruction for 2 weeks. A catheter was implanted into the bladder to record the IVP, and a balloon-fitted catheter was positioned in the abdominal cavity to record the IAP. PGE(2) was given intravesically to induce DO. Detrusor pressure (DP) was defined as the IVP corrected for IAP. RESULTS: Recorded as increases in IAP, all rats of both sexes exhibited abdominal straining during every void. In controls, a maximal IAP of 6.0 +/- 1.4 cmH(2)O (range 3-15 cmH(2)O) was registered (n = 12) at the time of the flow pressure (FP). Intravesical administration of PGE(2) or BOO did not affect the IAP at basal pressure, FP or micturition pressure. Changes in IAP due to movement or non-voiding-related straining were subtracted from IVP to generate DP and to visualize DO after BOO or intravesical PGE(2). CONCLUSIONS: The conscious rat uses abdominal straining during voiding, and maximal IAP is recorded at the onset of urinary flow. Simultaneous registration of IAP and IVP during the micturition cycle in conscious rats is a convenient method for accurate quantification of pressures inside the bladder and for studying "true" DO without interference from movement artifacts. Copyright 2007 Wiley-Liss, Inc}},
author = {{Lee, Tack and Andersson, Karl-Erik and Streng, Tomi and Hedlund, Petter}},
issn = {{0733-2467}},
language = {{eng}},
number = {{1}},
pages = {{88--95}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Neurourology and Urodynamics}},
title = {{Simultaneous registration of intraabdominal and intravesical pressures during cystometry in conscious rats-Effects of bladder outlet obstruction and intravesical PGE(2).}},
url = {{http://dx.doi.org/10.1002/nau.20460}},
doi = {{10.1002/nau.20460}},
volume = {{27}},
year = {{2008}},
}