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Simultaneous registration of intraabdominal and intravesical pressures during cystometry in conscious rats-Effects of bladder outlet obstruction and intravesical PGE(2).

Lee, Tack LU ; Andersson, Karl-Erik LU ; Streng, Tomi LU and Hedlund, Petter LU (2008) In Neurourology and Urodynamics 27(1). p.88-95
Abstract
AIMS: A method was developed and evaluated to simultaneously register intraabdominal pressure (IAP) and intravesical pressure (IVP) during cystometry in conscious rats. In addition, IAP and IVP were recorded in rats with experimental detrusor overactivity (DO). METHODS: Sprague Dawley rats (n = 24) were used. Six female rats were subjected to partial bladder outlet obstruction for 2 weeks. A catheter was implanted into the bladder to record the IVP, and a balloon-fitted catheter was positioned in the abdominal cavity to record the IAP. PGE(2) was given intravesically to induce DO. Detrusor pressure (DP) was defined as the IVP corrected for IAP. RESULTS: Recorded as increases in IAP, all rats of both sexes exhibited abdominal straining... (More)
AIMS: A method was developed and evaluated to simultaneously register intraabdominal pressure (IAP) and intravesical pressure (IVP) during cystometry in conscious rats. In addition, IAP and IVP were recorded in rats with experimental detrusor overactivity (DO). METHODS: Sprague Dawley rats (n = 24) were used. Six female rats were subjected to partial bladder outlet obstruction for 2 weeks. A catheter was implanted into the bladder to record the IVP, and a balloon-fitted catheter was positioned in the abdominal cavity to record the IAP. PGE(2) was given intravesically to induce DO. Detrusor pressure (DP) was defined as the IVP corrected for IAP. RESULTS: Recorded as increases in IAP, all rats of both sexes exhibited abdominal straining during every void. In controls, a maximal IAP of 6.0 +/- 1.4 cmH(2)O (range 3-15 cmH(2)O) was registered (n = 12) at the time of the flow pressure (FP). Intravesical administration of PGE(2) or BOO did not affect the IAP at basal pressure, FP or micturition pressure. Changes in IAP due to movement or non-voiding-related straining were subtracted from IVP to generate DP and to visualize DO after BOO or intravesical PGE(2). CONCLUSIONS: The conscious rat uses abdominal straining during voiding, and maximal IAP is recorded at the onset of urinary flow. Simultaneous registration of IAP and IVP during the micturition cycle in conscious rats is a convenient method for accurate quantification of pressures inside the bladder and for studying "true" DO without interference from movement artifacts. Copyright 2007 Wiley-Liss, Inc (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Neurourology and Urodynamics
volume
27
issue
1
pages
88 - 95
publisher
John Wiley & Sons
external identifiers
  • wos:000252241100016
  • scopus:37849045528
ISSN
0733-2467
DOI
10.1002/nau.20460
language
English
LU publication?
yes
id
3b4a7e26-e099-40ed-adb7-36929cb03221 (old id 539823)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17565725&dopt=Abstract
date added to LUP
2009-07-27 12:09:27
date last changed
2017-08-27 04:07:29
@article{3b4a7e26-e099-40ed-adb7-36929cb03221,
  abstract     = {AIMS: A method was developed and evaluated to simultaneously register intraabdominal pressure (IAP) and intravesical pressure (IVP) during cystometry in conscious rats. In addition, IAP and IVP were recorded in rats with experimental detrusor overactivity (DO). METHODS: Sprague Dawley rats (n = 24) were used. Six female rats were subjected to partial bladder outlet obstruction for 2 weeks. A catheter was implanted into the bladder to record the IVP, and a balloon-fitted catheter was positioned in the abdominal cavity to record the IAP. PGE(2) was given intravesically to induce DO. Detrusor pressure (DP) was defined as the IVP corrected for IAP. RESULTS: Recorded as increases in IAP, all rats of both sexes exhibited abdominal straining during every void. In controls, a maximal IAP of 6.0 +/- 1.4 cmH(2)O (range 3-15 cmH(2)O) was registered (n = 12) at the time of the flow pressure (FP). Intravesical administration of PGE(2) or BOO did not affect the IAP at basal pressure, FP or micturition pressure. Changes in IAP due to movement or non-voiding-related straining were subtracted from IVP to generate DP and to visualize DO after BOO or intravesical PGE(2). CONCLUSIONS: The conscious rat uses abdominal straining during voiding, and maximal IAP is recorded at the onset of urinary flow. Simultaneous registration of IAP and IVP during the micturition cycle in conscious rats is a convenient method for accurate quantification of pressures inside the bladder and for studying "true" DO without interference from movement artifacts. Copyright 2007 Wiley-Liss, Inc},
  author       = {Lee, Tack and Andersson, Karl-Erik and Streng, Tomi and Hedlund, Petter},
  issn         = {0733-2467},
  language     = {eng},
  number       = {1},
  pages        = {88--95},
  publisher    = {John Wiley & Sons},
  series       = {Neurourology and Urodynamics},
  title        = {Simultaneous registration of intraabdominal and intravesical pressures during cystometry in conscious rats-Effects of bladder outlet obstruction and intravesical PGE(2).},
  url          = {http://dx.doi.org/10.1002/nau.20460},
  volume       = {27},
  year         = {2008},
}