Roles of adiponectin and leptin signaling-related micrornas in the preventive effects of calorie restriction in mammary tumor development
(2021) In Applied Physiology, Nutrition and Metabolism 46(8). p.866-876- Abstract
Calorie restriction (CR) is suggested to prevent the development of mammary tumors (MTs); however, the mechanism remains to be clarified. We aimed to determine the microRNA (miRNA) profile in mice applied to 2 different CR protocols; chronic (CCR) and intermittent (ICR) and follow the MT development. In addition, the roles of miRNAs involved in adiponectin and/or leptin signaling pathways were investigated. Mice were divided into 3 groups: ad-libitum (AL), CCR, or ICR, which comprised 3 weeks of AL feeding followed by 1 week of 60% CR in a cyclic manner. Blood and tissue collection were performed at weeks 10, 17/18, 49/50 and 81/82. Long-term CCR provided better protection compared with ICR for MT development with a delay in the MT... (More)
Calorie restriction (CR) is suggested to prevent the development of mammary tumors (MTs); however, the mechanism remains to be clarified. We aimed to determine the microRNA (miRNA) profile in mice applied to 2 different CR protocols; chronic (CCR) and intermittent (ICR) and follow the MT development. In addition, the roles of miRNAs involved in adiponectin and/or leptin signaling pathways were investigated. Mice were divided into 3 groups: ad-libitum (AL), CCR, or ICR, which comprised 3 weeks of AL feeding followed by 1 week of 60% CR in a cyclic manner. Blood and tissue collection were performed at weeks 10, 17/18, 49/50 and 81/82. Long-term CCR provided better protection compared with ICR for MT development with a delay in the MT occurrence. Adiponectin expression in mammary fat pad were significantly higher in CCR group compared with AL. Using GeneChip Array, 250 of 3195 miRNAs were differentially expressed among the dietary groups. Thirteen of 250 miRNAs were related to adiponectin and/or leptin signaling genes. Results were verified by reverse transcription polymerase chain reaction. Specifically, miR-326-3p, miR-500-3p and miR-129-5p, which are related to adiponectin and/or leptin signaling, may play important roles in the preventive effects of CR in MT development and in ageing. Thus, these miRNAs might be putative biomarkers to target for diagnostic and treatment purposes. Novelty: Type of CR and micro RNA interaction is related to ageing. miR-326-3p, miR-500-3p and miR-129-5p expression levels were differentially expressed in MT development and in ageing. The genes associated with adiponectin and/or leptin signaling pathways are regulated by certain miRNAs in the protective effects of CR.
(Less)
- author
- organization
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Adipokines, Ageing, Intermittent calorie restriction, Mammary tumor, MiRNA
- in
- Applied Physiology, Nutrition and Metabolism
- volume
- 46
- issue
- 8
- pages
- 11 pages
- publisher
- National Research Council Canada
- external identifiers
-
- scopus:85103834591
- pmid:33493087
- ISSN
- 1715-5312
- DOI
- 10.1139/apnm-2020-1000
- language
- English
- LU publication?
- yes
- id
- 53ab7581-0be5-4204-9f5b-d68875256d38
- date added to LUP
- 2022-05-12 08:45:47
- date last changed
- 2024-04-18 08:04:04
@article{53ab7581-0be5-4204-9f5b-d68875256d38,
abstract = {{<p>Calorie restriction (CR) is suggested to prevent the development of mammary tumors (MTs); however, the mechanism remains to be clarified. We aimed to determine the microRNA (miRNA) profile in mice applied to 2 different CR protocols; chronic (CCR) and intermittent (ICR) and follow the MT development. In addition, the roles of miRNAs involved in adiponectin and/or leptin signaling pathways were investigated. Mice were divided into 3 groups: ad-libitum (AL), CCR, or ICR, which comprised 3 weeks of AL feeding followed by 1 week of 60% CR in a cyclic manner. Blood and tissue collection were performed at weeks 10, 17/18, 49/50 and 81/82. Long-term CCR provided better protection compared with ICR for MT development with a delay in the MT occurrence. Adiponectin expression in mammary fat pad were significantly higher in CCR group compared with AL. Using GeneChip Array, 250 of 3195 miRNAs were differentially expressed among the dietary groups. Thirteen of 250 miRNAs were related to adiponectin and/or leptin signaling genes. Results were verified by reverse transcription polymerase chain reaction. Specifically, miR-326-3p, miR-500-3p and miR-129-5p, which are related to adiponectin and/or leptin signaling, may play important roles in the preventive effects of CR in MT development and in ageing. Thus, these miRNAs might be putative biomarkers to target for diagnostic and treatment purposes. Novelty: Type of CR and micro RNA interaction is related to ageing. miR-326-3p, miR-500-3p and miR-129-5p expression levels were differentially expressed in MT development and in ageing. The genes associated with adiponectin and/or leptin signaling pathways are regulated by certain miRNAs in the protective effects of CR.</p>}},
author = {{Dogan, Soner and Cicekdal, Munevver B. and Ozorhan, Umit and Karabiyik, Goktug and Kazan, Busra T. and Ekici, Isin D. and Yilmaz, Bayram and Demirel, Pinar B. and Coban, Ilker and Tuysuz, Emre Can and Kuskucu, Aysegul and Bayrak, Omer F. and Cleary, Margot P. and Tuna, Bilge G.}},
issn = {{1715-5312}},
keywords = {{Adipokines; Ageing; Intermittent calorie restriction; Mammary tumor; MiRNA}},
language = {{eng}},
number = {{8}},
pages = {{866--876}},
publisher = {{National Research Council Canada}},
series = {{Applied Physiology, Nutrition and Metabolism}},
title = {{Roles of adiponectin and leptin signaling-related micrornas in the preventive effects of calorie restriction in mammary tumor development}},
url = {{http://dx.doi.org/10.1139/apnm-2020-1000}},
doi = {{10.1139/apnm-2020-1000}},
volume = {{46}},
year = {{2021}},
}