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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ertürk, Gizem LU and Lood, Rolf LU (2018) In Journal of Visualized Experiments
Abstract
The ability to detect and quantitate biomolecules in complex solutions has always been highly sought-after within natural science; being usedfor the detection of biomarkers, contaminants, and other molecules of interest. A commonly used technique for this purpose is the Enzyme-linked Immunosorbent Assay (ELISA), where often one antibody is directed towards a specific target molecule, and a second labeled antibodyis used for the detection of the primary antibody, allowing for the absolute quantification of the biomolecule under study. However, the usageof antibodies as recognition elements limits the robustness of the method; as does the need of using labeled molecules. To overcome theselimitations, molecular imprinting has been... (More)
The ability to detect and quantitate biomolecules in complex solutions has always been highly sought-after within natural science; being usedfor the detection of biomarkers, contaminants, and other molecules of interest. A commonly used technique for this purpose is the Enzyme-linked Immunosorbent Assay (ELISA), where often one antibody is directed towards a specific target molecule, and a second labeled antibodyis used for the detection of the primary antibody, allowing for the absolute quantification of the biomolecule under study. However, the usageof antibodies as recognition elements limits the robustness of the method; as does the need of using labeled molecules. To overcome theselimitations, molecular imprinting has been implemented, creating artificial recognition sites complementary to the template molecule, andobsoleting the necessity of using antibodies for initial binding. Further, for even higher sensitivity, the secondary labeled antibody can be replacedby biosensors relying on the capacitance for the quantification of the target molecule. In this protocol, we describe a method to rapidly and label-free detect and quantitate low-abundant biomolecules (proteins and viruses) in complex samples, with a sensitivity that is significantly better thancommonly used detection systems such as the ELISA. This is all mediated by molecular imprinting in combination with a capacitance biosensor (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Visualized Experiments
issue
132
publisher
JoVE
external identifiers
  • scopus:85042449311
ISSN
1940-087X
DOI
10.3791/57208
language
English
LU publication?
yes
id
53bcbf65-afd4-455b-9310-71efe788a36c
alternative location
https://www.jove.com/video/57208/ultrasensitive-detection-biomarkers-using-molecular-imprinting-based
date added to LUP
2018-03-05 08:21:26
date last changed
2018-06-05 03:00:01
@article{53bcbf65-afd4-455b-9310-71efe788a36c,
  abstract     = {The ability to detect and quantitate biomolecules in complex solutions has always been highly sought-after within natural science; being usedfor the detection of biomarkers, contaminants, and other molecules of interest. A commonly used technique for this purpose is the Enzyme-linked Immunosorbent Assay (ELISA), where often one antibody is directed towards a specific target molecule, and a second labeled antibodyis used for the detection of the primary antibody, allowing for the absolute quantification of the biomolecule under study. However, the usageof antibodies as recognition elements limits the robustness of the method; as does the need of using labeled molecules. To overcome theselimitations, molecular imprinting has been implemented, creating artificial recognition sites complementary to the template molecule, andobsoleting the necessity of using antibodies for initial binding. Further, for even higher sensitivity, the secondary labeled antibody can be replacedby biosensors relying on the capacitance for the quantification of the target molecule. In this protocol, we describe a method to rapidly and label-free detect and quantitate low-abundant biomolecules (proteins and viruses) in complex samples, with a sensitivity that is significantly better thancommonly used detection systems such as the ELISA. This is all mediated by molecular imprinting in combination with a capacitance biosensor},
  articleno    = {e57208},
  author       = {Ertürk, Gizem and Lood, Rolf},
  issn         = {1940-087X},
  language     = {eng},
  number       = {132},
  publisher    = {JoVE},
  series       = {Journal of Visualized Experiments},
  title        = {Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor},
  url          = {http://dx.doi.org/10.3791/57208},
  year         = {2018},
}