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Rel B is an early marker of autoimmune islet inflammation in the BioBreeding (BB) rat

Bieg, Sabine; Simonson, William T; Ellefsen, Kristian and Lernmark, Åke LU (2000) In Pancreas 20(1). p.47-54
Abstract

Because the development of insulitis and diabetes is predictable in Lyp/Lyp congenic BB rats, we have characterized early islet inflammation in these rats to determine the cell subsets involved in the onset of autoimmune insulitis. Pancreas sections from prediabetic Lyp/Lyp, Lyp/+ and +/+ rats were analyzed by immunohistochemistry. We found W3/25+ cells in the exo- and endocrine tissue from all three genotypes, but intraislet insulitis was never found in Lyp/+ or +/+ rats. The onset of massive, intraislet B- and T-cell infiltration in Lyp/Lyp rats was preceded by Rel B+ cells in and around the islets, followed by ED1+ monocytes/macrophages. Rel B+ cells were more frequent in the parafollicular cortex of pancreatic lymph nodes from... (More)

Because the development of insulitis and diabetes is predictable in Lyp/Lyp congenic BB rats, we have characterized early islet inflammation in these rats to determine the cell subsets involved in the onset of autoimmune insulitis. Pancreas sections from prediabetic Lyp/Lyp, Lyp/+ and +/+ rats were analyzed by immunohistochemistry. We found W3/25+ cells in the exo- and endocrine tissue from all three genotypes, but intraislet insulitis was never found in Lyp/+ or +/+ rats. The onset of massive, intraislet B- and T-cell infiltration in Lyp/Lyp rats was preceded by Rel B+ cells in and around the islets, followed by ED1+ monocytes/macrophages. Rel B+ cells were more frequent in the parafollicular cortex of pancreatic lymph nodes from Lyp/Lyp than from Lyp/+ and +/+ rats. In the Lyp/Lyp thymus, we found significantly increased expression of IL-12p40 messenger RNA (mRNA; p < 0.001), located in the Rel B-protein-rich corticomedullary junction. The NF-κB/Rel B complex specifically transactivates genes involved in antigen presentation in dendritic cells. Rel B+ cells in the islets may therefore mark the onset of autoimmune insulitis and antigen-specific activation of autoreactive T cells in the lymph nodes of diabetes prone Lyp/Lyp BB rats. In the thymus, Rel B+ cells may support the Lyp-dependent development of self-reactive thymocytes by activation of cytokine expression.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Antigen-presenting cell, Congenic, Dendritic cell, Diabetes, Pancreas, T cell
in
Pancreas
volume
20
issue
1
pages
8 pages
publisher
Lippincott Williams & Wilkins
external identifiers
  • scopus:0033957431
ISSN
0885-3177
DOI
10.1097/00006676-200001000-00007
language
English
LU publication?
no
id
53f0b0d1-8368-4d4b-b52c-caec02257fd9
date added to LUP
2017-09-06 15:21:33
date last changed
2017-09-06 15:21:33
@article{53f0b0d1-8368-4d4b-b52c-caec02257fd9,
  abstract     = {<p>Because the development of insulitis and diabetes is predictable in Lyp/Lyp congenic BB rats, we have characterized early islet inflammation in these rats to determine the cell subsets involved in the onset of autoimmune insulitis. Pancreas sections from prediabetic Lyp/Lyp, Lyp/+ and +/+ rats were analyzed by immunohistochemistry. We found W3/25+ cells in the exo- and endocrine tissue from all three genotypes, but intraislet insulitis was never found in Lyp/+ or +/+ rats. The onset of massive, intraislet B- and T-cell infiltration in Lyp/Lyp rats was preceded by Rel B+ cells in and around the islets, followed by ED1+ monocytes/macrophages. Rel B+ cells were more frequent in the parafollicular cortex of pancreatic lymph nodes from Lyp/Lyp than from Lyp/+ and +/+ rats. In the Lyp/Lyp thymus, we found significantly increased expression of IL-12p40 messenger RNA (mRNA; p &lt; 0.001), located in the Rel B-protein-rich corticomedullary junction. The NF-κB/Rel B complex specifically transactivates genes involved in antigen presentation in dendritic cells. Rel B+ cells in the islets may therefore mark the onset of autoimmune insulitis and antigen-specific activation of autoreactive T cells in the lymph nodes of diabetes prone Lyp/Lyp BB rats. In the thymus, Rel B+ cells may support the Lyp-dependent development of self-reactive thymocytes by activation of cytokine expression.</p>},
  author       = {Bieg, Sabine and Simonson, William T and Ellefsen, Kristian and Lernmark, Åke},
  issn         = {0885-3177},
  keyword      = {Antigen-presenting cell,Congenic,Dendritic cell,Diabetes,Pancreas,T cell},
  language     = {eng},
  number       = {1},
  pages        = {47--54},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Pancreas},
  title        = {Rel B is an early marker of autoimmune islet inflammation in the BioBreeding (BB) rat},
  url          = {http://dx.doi.org/10.1097/00006676-200001000-00007},
  volume       = {20},
  year         = {2000},
}