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The influence of the shape of Au nanoparticles on the catalytic current of fructose dehydrogenase

Bollella, Paolo ; Hibino, Yuya ; Conejo-Valverde, Paolo ; Soto-Cruz, Jackeline ; Bergueiro, Julián ; Calderón, Marcelo ; Rojas-Carrillo, Oscar ; Kano, Kenji and Gorton, Lo LU (2019) In Analytical and Bioanalytical Chemistry 411(29). p.7645-7657
Abstract

Graphite electrodes were modified with triangular (AuNTrs) or spherical (AuNPs) nanoparticles and further modified with fructose dehydrogenase (FDH). The present study reports the effect of the shape of these nanoparticles (NPs) on the catalytic current of immobilized FDH pointing out the different contributions on the mass transfer–limited and kinetically limited currents. The influence of the shape of the NPs on the mass transfer–limited and the kinetically limited current has been proved by using two different methods: a rotating disk electrode (RDE) and an electrode mounted in a wall jet flow-through electrochemical cell attached to a flow system. The advantages of using the wall jet flow system compared with the RDE system for... (More)

Graphite electrodes were modified with triangular (AuNTrs) or spherical (AuNPs) nanoparticles and further modified with fructose dehydrogenase (FDH). The present study reports the effect of the shape of these nanoparticles (NPs) on the catalytic current of immobilized FDH pointing out the different contributions on the mass transfer–limited and kinetically limited currents. The influence of the shape of the NPs on the mass transfer–limited and the kinetically limited current has been proved by using two different methods: a rotating disk electrode (RDE) and an electrode mounted in a wall jet flow-through electrochemical cell attached to a flow system. The advantages of using the wall jet flow system compared with the RDE system for kinetic investigations are as follows: no need to account for substrate consumption, especially in the case of desorption of enzyme, and studies of product-inhibited enzymes. The comparison reveals that virtually identical results can be obtained using either of the two techniques. The heterogeneous electron transfer (ET) rate constants (kS) were found to be 3.8 ± 0.3 s−1 and 0.9 ± 0.1 s−1, for triangular and spherical NPs, respectively. The improvement observed for the electrode modified with AuNTrs suggests a more effective enzyme-NP interaction, which can allocate a higher number of enzyme molecules on the electrode surface. 

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Direct electron transfer (DET), Fructose dehydrogenase (FDH), Gold nanoparticles (AuNPs), Gold nanotriangles (AuNTrs), Nanoparticle shape
in
Analytical and Bioanalytical Chemistry
volume
411
issue
29
pages
13 pages
publisher
Springer
external identifiers
  • scopus:85069668929
  • pmid:31286179
ISSN
1618-2642
DOI
10.1007/s00216-019-01944-6
language
English
LU publication?
yes
id
53f472ed-0060-4ae6-b1a4-4abca33153c1
date added to LUP
2019-08-09 16:15:20
date last changed
2023-01-23 18:49:07
@article{53f472ed-0060-4ae6-b1a4-4abca33153c1,
  abstract     = {{<p>Graphite electrodes were modified with triangular (AuNTrs) or spherical (AuNPs) nanoparticles and further modified with fructose dehydrogenase (FDH). The present study reports the effect of the shape of these nanoparticles (NPs) on the catalytic current of immobilized FDH pointing out the different contributions on the mass transfer–limited and kinetically limited currents. The influence of the shape of the NPs on the mass transfer–limited and the kinetically limited current has been proved by using two different methods: a rotating disk electrode (RDE) and an electrode mounted in a wall jet flow-through electrochemical cell attached to a flow system. The advantages of using the wall jet flow system compared with the RDE system for kinetic investigations are as follows: no need to account for substrate consumption, especially in the case of desorption of enzyme, and studies of product-inhibited enzymes. The comparison reveals that virtually identical results can be obtained using either of the two techniques. The heterogeneous electron transfer (ET) rate constants (k<sub>S</sub>) were found to be 3.8 ± 0.3 s<sup>−1</sup> and 0.9 ± 0.1 s<sup>−1</sup>, for triangular and spherical NPs, respectively. The improvement observed for the electrode modified with AuNTrs suggests a more effective enzyme-NP interaction, which can allocate a higher number of enzyme molecules on the electrode surface. </p>}},
  author       = {{Bollella, Paolo and Hibino, Yuya and Conejo-Valverde, Paolo and Soto-Cruz, Jackeline and Bergueiro, Julián and Calderón, Marcelo and Rojas-Carrillo, Oscar and Kano, Kenji and Gorton, Lo}},
  issn         = {{1618-2642}},
  keywords     = {{Direct electron transfer (DET); Fructose dehydrogenase (FDH); Gold nanoparticles (AuNPs); Gold nanotriangles (AuNTrs); Nanoparticle shape}},
  language     = {{eng}},
  number       = {{29}},
  pages        = {{7645--7657}},
  publisher    = {{Springer}},
  series       = {{Analytical and Bioanalytical Chemistry}},
  title        = {{The influence of the shape of Au nanoparticles on the catalytic current of fructose dehydrogenase}},
  url          = {{http://dx.doi.org/10.1007/s00216-019-01944-6}},
  doi          = {{10.1007/s00216-019-01944-6}},
  volume       = {{411}},
  year         = {{2019}},
}