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Alterations in Serum MicroRNA Profile during Hemodialysis-Potential Biological Implications

Trzybulska, Dorota LU ; Eckersten, Dag LU ; Giwercman, Aleksander LU ; Christensson, Anders LU and Tsatsanis, Christos LU (2018) In Cellular Physiology and Biochemistry 46(2). p.793-801
Abstract

Background/Aims: Hemodialysis implies significant alterations in the profile of serum components. microRNAs (miRNAs) are present in the human serum and are considered to target distant tissues where they can regulate gene expression, thus affecting homeostasis. Whether hemodialysis alters the profile of miRNAs in the serum is not known. Methods: miRNA profiling in serum samples collected before and after hemodialysis was performed using miRNA qPCR arrays. The results were subsequently validated in an independent group of 10 hemodialyzed men. miRWalk database was used to identify mRNAs targeted by the miRNAs the levels of which changed after hemodialysis. The list of mRNAs was analyzed using the DAVID and PANTHER classification systems... (More)

Background/Aims: Hemodialysis implies significant alterations in the profile of serum components. microRNAs (miRNAs) are present in the human serum and are considered to target distant tissues where they can regulate gene expression, thus affecting homeostasis. Whether hemodialysis alters the profile of miRNAs in the serum is not known. Methods: miRNA profiling in serum samples collected before and after hemodialysis was performed using miRNA qPCR arrays. The results were subsequently validated in an independent group of 10 hemodialyzed men. miRWalk database was used to identify mRNAs targeted by the miRNAs the levels of which changed after hemodialysis. The list of mRNAs was analyzed using the DAVID and PANTHER classification systems to identify pathways controlled by these miRNAs. Results: miRNA profiling showed that the levels of the majority of circulating miRNAs were increased at least two-fold (115 out of 179 tested) while the levels of only five miRNAs were found at least two-fold lower after hemodialysis. Validation study confirmed the majority of the array results. Bioinformatics analysis of validated and significantly upregulated miRNAs revealed that gonadotropin-releasing hormone receptor, cell cycle and cell pluripotency-related pathways were targeted. Conclusion: Hemodialysis alters serum miRNA expression profile and this alteration may result in disruption of pathways contributing to subfertility and increased risk for cancer development being pathologies associated with hemodialysis.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cancer, Comorbidities, Fertility, Hemodialysis, Inflammation, MiRNA
in
Cellular Physiology and Biochemistry
volume
46
issue
2
pages
9 pages
publisher
Karger
external identifiers
  • scopus:85046104239
ISSN
1015-8987
DOI
10.1159/000488737
language
English
LU publication?
yes
id
540a685e-387b-4906-89cb-eb7781335cf6
date added to LUP
2018-05-14 15:19:43
date last changed
2018-05-15 03:00:02
@article{540a685e-387b-4906-89cb-eb7781335cf6,
  abstract     = {<p>Background/Aims: Hemodialysis implies significant alterations in the profile of serum components. microRNAs (miRNAs) are present in the human serum and are considered to target distant tissues where they can regulate gene expression, thus affecting homeostasis. Whether hemodialysis alters the profile of miRNAs in the serum is not known. Methods: miRNA profiling in serum samples collected before and after hemodialysis was performed using miRNA qPCR arrays. The results were subsequently validated in an independent group of 10 hemodialyzed men. miRWalk database was used to identify mRNAs targeted by the miRNAs the levels of which changed after hemodialysis. The list of mRNAs was analyzed using the DAVID and PANTHER classification systems to identify pathways controlled by these miRNAs. Results: miRNA profiling showed that the levels of the majority of circulating miRNAs were increased at least two-fold (115 out of 179 tested) while the levels of only five miRNAs were found at least two-fold lower after hemodialysis. Validation study confirmed the majority of the array results. Bioinformatics analysis of validated and significantly upregulated miRNAs revealed that gonadotropin-releasing hormone receptor, cell cycle and cell pluripotency-related pathways were targeted. Conclusion: Hemodialysis alters serum miRNA expression profile and this alteration may result in disruption of pathways contributing to subfertility and increased risk for cancer development being pathologies associated with hemodialysis.</p>},
  author       = {Trzybulska, Dorota and Eckersten, Dag and Giwercman, Aleksander and Christensson, Anders and Tsatsanis, Christos},
  issn         = {1015-8987},
  keyword      = {Cancer,Comorbidities,Fertility,Hemodialysis,Inflammation,MiRNA},
  language     = {eng},
  month        = {04},
  number       = {2},
  pages        = {793--801},
  publisher    = {Karger},
  series       = {Cellular Physiology and Biochemistry},
  title        = {Alterations in Serum MicroRNA Profile during Hemodialysis-Potential Biological Implications},
  url          = {http://dx.doi.org/10.1159/000488737},
  volume       = {46},
  year         = {2018},
}