GARD : Genomic allergen rapid detection
(2017) p.393-403- Abstract
Genomic allergen rapid detection (GARD) is an in vitro assay for the prediction of skin sensitizing capacity of chemicals. The GARD assay monitors changes in the expression of 196 genes, termed the GARD prediction signature. In short, MUTZ-3 cells, representing a well-characterized in vitro model of human dendritic cells (DCs), are exposed to the test substances for 24 h. After RNA extraction and analysis of expression levels, compounds are predicted as either sensitizers or non-sensitizers by a support vector machine model. Due to high information content, the test can deliver novel insights into pathway utilization and potency. Based on blinded test evaluations, GARD has been shown to have an accuracy of 89%.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/540bbf6e-8901-42a7-b84b-32bf86574867
- author
- Lindstedt, Malin LU ; Zeller, Kathrin Stephanie LU ; Johansson, Henrik LU and Borrebaeck, Carl LU
- organization
- publishing date
- 2017
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Biomarker signatures, Dendritic cells, Human, In vitro assay, Sensitization
- host publication
- Alternatives for Dermal Toxicity Testing
- pages
- 393 - 403
- publisher
- Springer International Publishing
- external identifiers
-
- scopus:85042614031
- ISBN
- 9783319503530
- 9783319503516
- DOI
- 10.1007/978-3-319-50353-0_27
- language
- English
- LU publication?
- yes
- id
- 540bbf6e-8901-42a7-b84b-32bf86574867
- date added to LUP
- 2018-03-09 13:30:40
- date last changed
- 2024-10-26 02:48:05
@inbook{540bbf6e-8901-42a7-b84b-32bf86574867, abstract = {{<p>Genomic allergen rapid detection (GARD) is an in vitro assay for the prediction of skin sensitizing capacity of chemicals. The GARD assay monitors changes in the expression of 196 genes, termed the GARD prediction signature. In short, MUTZ-3 cells, representing a well-characterized in vitro model of human dendritic cells (DCs), are exposed to the test substances for 24 h. After RNA extraction and analysis of expression levels, compounds are predicted as either sensitizers or non-sensitizers by a support vector machine model. Due to high information content, the test can deliver novel insights into pathway utilization and potency. Based on blinded test evaluations, GARD has been shown to have an accuracy of 89%.</p>}}, author = {{Lindstedt, Malin and Zeller, Kathrin Stephanie and Johansson, Henrik and Borrebaeck, Carl}}, booktitle = {{Alternatives for Dermal Toxicity Testing}}, isbn = {{9783319503530}}, keywords = {{Biomarker signatures; Dendritic cells; Human; In vitro assay; Sensitization}}, language = {{eng}}, pages = {{393--403}}, publisher = {{Springer International Publishing}}, title = {{GARD : Genomic allergen rapid detection}}, url = {{http://dx.doi.org/10.1007/978-3-319-50353-0_27}}, doi = {{10.1007/978-3-319-50353-0_27}}, year = {{2017}}, }