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GARD : Genomic allergen rapid detection

Lindstedt, Malin LU ; Zeller, Kathrin Stephanie LU orcid ; Johansson, Henrik LU and Borrebaeck, Carl LU (2017) p.393-403
Abstract

Genomic allergen rapid detection (GARD) is an in vitro assay for the prediction of skin sensitizing capacity of chemicals. The GARD assay monitors changes in the expression of 196 genes, termed the GARD prediction signature. In short, MUTZ-3 cells, representing a well-characterized in vitro model of human dendritic cells (DCs), are exposed to the test substances for 24 h. After RNA extraction and analysis of expression levels, compounds are predicted as either sensitizers or non-sensitizers by a support vector machine model. Due to high information content, the test can deliver novel insights into pathway utilization and potency. Based on blinded test evaluations, GARD has been shown to have an accuracy of 89%.

Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Biomarker signatures, Dendritic cells, Human, In vitro assay, Sensitization
host publication
Alternatives for Dermal Toxicity Testing
pages
393 - 403
publisher
Springer International Publishing
external identifiers
  • scopus:85042614031
ISBN
9783319503530
9783319503516
DOI
10.1007/978-3-319-50353-0_27
language
English
LU publication?
yes
id
540bbf6e-8901-42a7-b84b-32bf86574867
date added to LUP
2018-03-09 13:30:40
date last changed
2024-04-01 00:48:21
@inbook{540bbf6e-8901-42a7-b84b-32bf86574867,
  abstract     = {{<p>Genomic allergen rapid detection (GARD) is an in vitro assay for the prediction of skin sensitizing capacity of chemicals. The GARD assay monitors changes in the expression of 196 genes, termed the GARD prediction signature. In short, MUTZ-3 cells, representing a well-characterized in vitro model of human dendritic cells (DCs), are exposed to the test substances for 24 h. After RNA extraction and analysis of expression levels, compounds are predicted as either sensitizers or non-sensitizers by a support vector machine model. Due to high information content, the test can deliver novel insights into pathway utilization and potency. Based on blinded test evaluations, GARD has been shown to have an accuracy of 89%.</p>}},
  author       = {{Lindstedt, Malin and Zeller, Kathrin Stephanie and Johansson, Henrik and Borrebaeck, Carl}},
  booktitle    = {{Alternatives for Dermal Toxicity Testing}},
  isbn         = {{9783319503530}},
  keywords     = {{Biomarker signatures; Dendritic cells; Human; In vitro assay; Sensitization}},
  language     = {{eng}},
  pages        = {{393--403}},
  publisher    = {{Springer International Publishing}},
  title        = {{GARD : Genomic allergen rapid detection}},
  url          = {{http://dx.doi.org/10.1007/978-3-319-50353-0_27}},
  doi          = {{10.1007/978-3-319-50353-0_27}},
  year         = {{2017}},
}