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A novel serotonin-containing tuft cell subpopulation in mouse intestine

Cheng, Xiaowen LU ; Voss, Ulrikke LU and Ekblad, Eva LU (2019) In Cell and Tissue Research 376(2). p.189-197
Abstract

In this study, a novel subset of doublecortin-like kinase 1 (DCLK1)-immunoreactive (IR) tuft cells that also contain serotonin (5-hydroxytryptamine, 5HT) is described, in terms of their number, regional distribution, possible synthesis or reuptake of 5HT and proximity to 5-HT-containing enterochromaffin (EC) cells. The small intestine from C57BL/6J mice was divided into five segments while the large intestine was kept undivided. Double immunostaining was used to estimate numbers and topographic distribution of 5HT-IR (DCLK1/5HT) tuft cells and their possible expression of tryptophan hydroxylase (TPH) and serotonin transporter (SERT). Also, possible contacts between tuft cells and 5HT-IR EC cells were studied. In the small intestine, up... (More)

In this study, a novel subset of doublecortin-like kinase 1 (DCLK1)-immunoreactive (IR) tuft cells that also contain serotonin (5-hydroxytryptamine, 5HT) is described, in terms of their number, regional distribution, possible synthesis or reuptake of 5HT and proximity to 5-HT-containing enterochromaffin (EC) cells. The small intestine from C57BL/6J mice was divided into five segments while the large intestine was kept undivided. Double immunostaining was used to estimate numbers and topographic distribution of 5HT-IR (DCLK1/5HT) tuft cells and their possible expression of tryptophan hydroxylase (TPH) and serotonin transporter (SERT). Also, possible contacts between tuft cells and 5HT-IR EC cells were studied. In the small intestine, up to 80% of all tuft cells were identified as DCLK1/5HT-IR; in the large intestine, such cells were rare. The highest number of DCLK1/5HT-IR cells was found in the upper small intestine. The numbers of DCLK1/5HT-IR cells gradually decreased distally. DCLK1-IR tuft cells were not found to contain TPH, the rate-limiting enzyme in 5HT synthesis. SERT, the selective transporter for 5HT reuptake, could not convincingly be demonstrated in tuft cells. In villi and crypts, 3% and 10%, respectively, of all DCLK1-IR cells were in close proximity to EC cells. EC cells in close proximity to DCLK1-IR cells were, in villi and crypts, 3 and 8%, respectively. We conclude that DCLK1/5HT-IR cells constitute a novel subset of tuft cells that may have unique roles in the GI tract.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Enterochromaffin cell, Serotonin, Serotonin transporter, Tryptophan hydroxylase, Tuft cell
in
Cell and Tissue Research
volume
376
issue
2
pages
189 - 197
publisher
Springer
external identifiers
  • scopus:85060328579
ISSN
0302-766X
DOI
10.1007/s00441-018-02988-3
language
English
LU publication?
yes
id
54224ebe-aede-47f4-95af-0cde07aa675a
date added to LUP
2019-02-01 09:40:55
date last changed
2019-06-28 10:01:12
@article{54224ebe-aede-47f4-95af-0cde07aa675a,
  abstract     = {<p>In this study, a novel subset of doublecortin-like kinase 1 (DCLK1)-immunoreactive (IR) tuft cells that also contain serotonin (5-hydroxytryptamine, 5HT) is described, in terms of their number, regional distribution, possible synthesis or reuptake of 5HT and proximity to 5-HT-containing enterochromaffin (EC) cells. The small intestine from C57BL/6J mice was divided into five segments while the large intestine was kept undivided. Double immunostaining was used to estimate numbers and topographic distribution of 5HT-IR (DCLK1/5HT) tuft cells and their possible expression of tryptophan hydroxylase (TPH) and serotonin transporter (SERT). Also, possible contacts between tuft cells and 5HT-IR EC cells were studied. In the small intestine, up to 80% of all tuft cells were identified as DCLK1/5HT-IR; in the large intestine, such cells were rare. The highest number of DCLK1/5HT-IR cells was found in the upper small intestine. The numbers of DCLK1/5HT-IR cells gradually decreased distally. DCLK1-IR tuft cells were not found to contain TPH, the rate-limiting enzyme in 5HT synthesis. SERT, the selective transporter for 5HT reuptake, could not convincingly be demonstrated in tuft cells. In villi and crypts, 3% and 10%, respectively, of all DCLK1-IR cells were in close proximity to EC cells. EC cells in close proximity to DCLK1-IR cells were, in villi and crypts, 3 and 8%, respectively. We conclude that DCLK1/5HT-IR cells constitute a novel subset of tuft cells that may have unique roles in the GI tract.</p>},
  author       = {Cheng, Xiaowen and Voss, Ulrikke and Ekblad, Eva},
  issn         = {0302-766X},
  keyword      = {Enterochromaffin cell,Serotonin,Serotonin transporter,Tryptophan hydroxylase,Tuft cell},
  language     = {eng},
  month        = {01},
  number       = {2},
  pages        = {189--197},
  publisher    = {Springer},
  series       = {Cell and Tissue Research},
  title        = {A novel serotonin-containing tuft cell subpopulation in mouse intestine},
  url          = {http://dx.doi.org/10.1007/s00441-018-02988-3},
  volume       = {376},
  year         = {2019},
}