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Paralytic shellfish toxin content is related to genomic sxtA4 copy number in Alexandrium minutum strains.

Stüken, Anke; Riobó, Pilar; Franco, José; Jakobsen, Kjetill S; Guillou, Laure and Figueroa, Rosa LU (2015) In Frontiers in Microbiology 6.
Abstract
Dinoflagellates are microscopic aquatic eukaryotes with huge genomes and an unusual cell regulation. For example, most genes are present in numerous copies and all copies seem to be obligatorily transcribed. The consequence of the gene copy number (CPN) for final protein synthesis is, however, not clear. One such gene is sxtA, the starting gene of paralytic shellfish toxin (PST) synthesis. PSTs are small neurotoxic compounds that can accumulate in the food chain and cause serious poisoning incidences when ingested. They are produced by dinoflagellates of the genera Alexandrium, Gymnodium, and Pyrodinium. Here we investigated if the genomic CPN of sxtA4 is related to PST content in Alexandrium minutum cells. SxtA4 is the 4th domain of the... (More)
Dinoflagellates are microscopic aquatic eukaryotes with huge genomes and an unusual cell regulation. For example, most genes are present in numerous copies and all copies seem to be obligatorily transcribed. The consequence of the gene copy number (CPN) for final protein synthesis is, however, not clear. One such gene is sxtA, the starting gene of paralytic shellfish toxin (PST) synthesis. PSTs are small neurotoxic compounds that can accumulate in the food chain and cause serious poisoning incidences when ingested. They are produced by dinoflagellates of the genera Alexandrium, Gymnodium, and Pyrodinium. Here we investigated if the genomic CPN of sxtA4 is related to PST content in Alexandrium minutum cells. SxtA4 is the 4th domain of the sxtA gene and its presence is essential for PST synthesis in dinoflagellates. We used PST and genome size measurements as well as quantitative PCR to analyze sxtA4 CPN and toxin content in 15 A. minutum strains. Our results show a strong positive correlation between the sxtA4 CPN and the total amount of PST produced in actively growing A. minutum cells. This correlation was independent of the toxin profile produced, as long as the strain contained the genomic domains sxtA1 and sxtA4. (Less)
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Contribution to journal
publication status
published
subject
in
Frontiers in Microbiology
volume
6
publisher
Frontiers
external identifiers
  • pmid:25983733
  • wos:000355006000001
  • scopus:84931261459
ISSN
1664-302X
DOI
10.3389/fmicb.2015.00404
language
English
LU publication?
yes
id
0581eaac-70ed-4274-8a09-163fef7573ef (old id 5449093)
date added to LUP
2015-06-16 14:52:49
date last changed
2017-11-19 03:51:02
@article{0581eaac-70ed-4274-8a09-163fef7573ef,
  abstract     = {Dinoflagellates are microscopic aquatic eukaryotes with huge genomes and an unusual cell regulation. For example, most genes are present in numerous copies and all copies seem to be obligatorily transcribed. The consequence of the gene copy number (CPN) for final protein synthesis is, however, not clear. One such gene is sxtA, the starting gene of paralytic shellfish toxin (PST) synthesis. PSTs are small neurotoxic compounds that can accumulate in the food chain and cause serious poisoning incidences when ingested. They are produced by dinoflagellates of the genera Alexandrium, Gymnodium, and Pyrodinium. Here we investigated if the genomic CPN of sxtA4 is related to PST content in Alexandrium minutum cells. SxtA4 is the 4th domain of the sxtA gene and its presence is essential for PST synthesis in dinoflagellates. We used PST and genome size measurements as well as quantitative PCR to analyze sxtA4 CPN and toxin content in 15 A. minutum strains. Our results show a strong positive correlation between the sxtA4 CPN and the total amount of PST produced in actively growing A. minutum cells. This correlation was independent of the toxin profile produced, as long as the strain contained the genomic domains sxtA1 and sxtA4.},
  articleno    = {404},
  author       = {Stüken, Anke and Riobó, Pilar and Franco, José and Jakobsen, Kjetill S and Guillou, Laure and Figueroa, Rosa},
  issn         = {1664-302X},
  language     = {eng},
  publisher    = {Frontiers},
  series       = {Frontiers in Microbiology},
  title        = {Paralytic shellfish toxin content is related to genomic sxtA4 copy number in Alexandrium minutum strains.},
  url          = {http://dx.doi.org/10.3389/fmicb.2015.00404},
  volume       = {6},
  year         = {2015},
}