Interaction of Bacteroides fragilis and Bacteroides thetaiotaomicron with the kallikrein-kinin system
(2011) In Microbiology 157. p.2094-2105- Abstract
- Many bacterial pathogens interfere with the contact system (kallikrein kinin system) in human plasma. Activation of this system has two consequences: cleavage of high-molecular-mass kininogen (HK) resulting in release of the potent proinflammatory peptide bradykinin, and initiation of the intrinsic pathway of coagulation. In this study, two species of the Gram-negative anaerobic commensal organism Bacteroides, namely Bacteroides fragilis and Bacteroides thetaiotaomicron, were found to bind HK and fibrinogen, the major clotting protein, from human plasma as shown by immunoelectron microscopy and Western blot analysis. In addition, these Bacteroides species were capable of activating the contact system at its surface leading to a significant... (More)
- Many bacterial pathogens interfere with the contact system (kallikrein kinin system) in human plasma. Activation of this system has two consequences: cleavage of high-molecular-mass kininogen (HK) resulting in release of the potent proinflammatory peptide bradykinin, and initiation of the intrinsic pathway of coagulation. In this study, two species of the Gram-negative anaerobic commensal organism Bacteroides, namely Bacteroides fragilis and Bacteroides thetaiotaomicron, were found to bind HK and fibrinogen, the major clotting protein, from human plasma as shown by immunoelectron microscopy and Western blot analysis. In addition, these Bacteroides species were capable of activating the contact system at its surface leading to a significant prolongation of the intrinsic coagulation time and also to the release of bradykinin. Members of the genus Bacteroides have been known to act as opportunistic pathogens outside the gut, with B. fragilis being the most common isolate from clinical infections, such as intra-abdominal abscesses and bacteraemia. The present results thus provide more insight into how Bacteroides species cause infection. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2072016
- author
- Murphy, Elizabeth LU ; Mörgelin, Matthias LU ; Cooney, Jakki C. and Frick, Inga-Maria LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Microbiology
- volume
- 157
- pages
- 2094 - 2105
- publisher
- MAIK Nauka/Interperiodica
- external identifiers
-
- wos:000293204000021
- pmid:21527472
- scopus:79959993374
- pmid:21527472
- ISSN
- 1465-2080
- DOI
- 10.1099/mic.0.046862-0
- language
- English
- LU publication?
- yes
- id
- 544a27f2-ed0f-4aff-bb7f-0ddb0529ec57 (old id 2072016)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21527472?dopt=Abstract
- date added to LUP
- 2016-04-01 10:23:58
- date last changed
- 2022-01-25 22:52:24
@article{544a27f2-ed0f-4aff-bb7f-0ddb0529ec57,
abstract = {{Many bacterial pathogens interfere with the contact system (kallikrein kinin system) in human plasma. Activation of this system has two consequences: cleavage of high-molecular-mass kininogen (HK) resulting in release of the potent proinflammatory peptide bradykinin, and initiation of the intrinsic pathway of coagulation. In this study, two species of the Gram-negative anaerobic commensal organism Bacteroides, namely Bacteroides fragilis and Bacteroides thetaiotaomicron, were found to bind HK and fibrinogen, the major clotting protein, from human plasma as shown by immunoelectron microscopy and Western blot analysis. In addition, these Bacteroides species were capable of activating the contact system at its surface leading to a significant prolongation of the intrinsic coagulation time and also to the release of bradykinin. Members of the genus Bacteroides have been known to act as opportunistic pathogens outside the gut, with B. fragilis being the most common isolate from clinical infections, such as intra-abdominal abscesses and bacteraemia. The present results thus provide more insight into how Bacteroides species cause infection.}},
author = {{Murphy, Elizabeth and Mörgelin, Matthias and Cooney, Jakki C. and Frick, Inga-Maria}},
issn = {{1465-2080}},
language = {{eng}},
pages = {{2094--2105}},
publisher = {{MAIK Nauka/Interperiodica}},
series = {{Microbiology}},
title = {{Interaction of Bacteroides fragilis and Bacteroides thetaiotaomicron with the kallikrein-kinin system}},
url = {{http://dx.doi.org/10.1099/mic.0.046862-0}},
doi = {{10.1099/mic.0.046862-0}},
volume = {{157}},
year = {{2011}},
}