Characterization of fibroblast phenotypes in human airway remodeling
(2005)- Abstract
- Wound healing that precedes scar formation may be unbalanced under pathological conditions leading to structural changes of the tissue. At the moment, the mechanisms behind this unbalance are unclear. When these changes occur in internal organs such as the lung, they may result in organ dysfunction with fatal consequences for the patient. This phenomenon is defined as airway remodeling, and occurs in several pulmonary disorders such as asthma, chronic obstructive pulmonary disease (COPD), idiopathic pulmonary fibrosis (IPF), and systemic sclerosis (SSc). Airway remodeling is characterized by a deposition of extracelullar matrix (ECM) components such as collagens, fibronectins, and proteoglycans.
It is generally accepted... (More) - Wound healing that precedes scar formation may be unbalanced under pathological conditions leading to structural changes of the tissue. At the moment, the mechanisms behind this unbalance are unclear. When these changes occur in internal organs such as the lung, they may result in organ dysfunction with fatal consequences for the patient. This phenomenon is defined as airway remodeling, and occurs in several pulmonary disorders such as asthma, chronic obstructive pulmonary disease (COPD), idiopathic pulmonary fibrosis (IPF), and systemic sclerosis (SSc). Airway remodeling is characterized by a deposition of extracelullar matrix (ECM) components such as collagens, fibronectins, and proteoglycans.
It is generally accepted that activated fibroblasts play a key role in airway remodeling due to their ability to regulate the ECM turnover, which include the production and breakdown of connective tissue. When fibroblasts are activated they acquire a myofibroblast phenotype that is characterized by an increased production of ECM and contractility. Myofibroblasts are considered important target cells when studying airway remodeling and further characterization of these cells may lead to improved insights into the mechanisms behind this process. This is important since a majority of the therapies for these disorders are limited towards inflammatory response rather than tissue remodeling.
This thesis was aimed to characterize myofibroblast phenotypes and to elucidate the origin of these cells in airway remodeling, focused on tissue mainly derived from patients with mild asthma. To identify potential markers involved in disease mechanisms, methods used to study proteoglycan profiles and protein expression pattern (Proteomics) have been utilized. These topics, including the basics of airway remodeling, will be reviewed in the following sections. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/545559
- author
- Larsen, Kristoffer LU
- supervisor
- opponent
-
- MD, PhD Harrison, Kim, University of Swansea, Wales, UK
- organization
- publishing date
- 2005
- type
- Thesis
- publication status
- published
- subject
- keywords
- Medicine (human and vertebrates), Medicin (människa och djur), remodeling, General biomedical sciences, Biomedicinska vetenskaper (allmänt), fibrocytes, proteoglycans, Asthma, fibroblasts
- pages
- 117 pages
- publisher
- Department of Experimental Medical Science, Lund Univeristy
- defense location
- GK-salen, BMC, Sölvegatan 19, Lund
- defense date
- 2005-11-18 09:15:00
- ISBN
- 91-85481-16-5
- language
- English
- LU publication?
- yes
- additional info
- Johan Malmström, Kristoffer Larsen, Lennart Hansson, Claes-Göran Löfdahl, Ole Nörregaard-Jensen, György Marko-Varja and Gunilla Westergren-Thorsson. 2002. Proteoglycan and proteome profiling of central human pulmonary fibrotic tissue utilizing a minituarized sample preparation: A feasibility study. Proteomics, vol Apr 2 pp 394-404.Kristoffer Larsen, Ellen Tufvesson, Johan Malmström, Matthias Mörgelin, Marie Wildt, Annika Andersson, Anna Lindström, Anders Malmström, Claes-Göran Löfdahl, György Marko-Varja, Leif Bjermer and Gunilla Westergren-Thorsson. 2004. Presence of activated mobile fibroblasts in bronchoalveolar lavage from patients with mild asthma. Am J Respir Crit Care Med., vol Nov pp 1049-56.Kristian Nihlberg, Kristoffer Larsen, Anna Hultgårdh-Nilsson, Anders Malmström, Claes-Göran Löfdahl, Leif Bjermer and Gunilla Westergren-Thorsson. . Fibrocytes in patients with mild asthma: A possible link to thickness of reticular basement membrane? Journal of Immunology, (submitted)Kristoffer Larsen, Johan Malmström, Marie Wildt, Lennart Hansson, Camilla Dahlqvist, György Marko-Varga, Leif Bjermer, Agneta Scheja and Gunilla Westergren-Thorsson. . Alternative splicing, motility and proteome expression in myofibroblasts in mild asthma and scleroderma. Respiratory Research, (submitted)
- id
- f51f7e2c-6c57-41c6-a0e2-b628f0606ffa (old id 545559)
- date added to LUP
- 2016-04-01 15:47:55
- date last changed
- 2023-04-18 19:51:03
@phdthesis{f51f7e2c-6c57-41c6-a0e2-b628f0606ffa, abstract = {{Wound healing that precedes scar formation may be unbalanced under pathological conditions leading to structural changes of the tissue. At the moment, the mechanisms behind this unbalance are unclear. When these changes occur in internal organs such as the lung, they may result in organ dysfunction with fatal consequences for the patient. This phenomenon is defined as airway remodeling, and occurs in several pulmonary disorders such as asthma, chronic obstructive pulmonary disease (COPD), idiopathic pulmonary fibrosis (IPF), and systemic sclerosis (SSc). Airway remodeling is characterized by a deposition of extracelullar matrix (ECM) components such as collagens, fibronectins, and proteoglycans.<br/><br> <br/><br> It is generally accepted that activated fibroblasts play a key role in airway remodeling due to their ability to regulate the ECM turnover, which include the production and breakdown of connective tissue. When fibroblasts are activated they acquire a myofibroblast phenotype that is characterized by an increased production of ECM and contractility. Myofibroblasts are considered important target cells when studying airway remodeling and further characterization of these cells may lead to improved insights into the mechanisms behind this process. This is important since a majority of the therapies for these disorders are limited towards inflammatory response rather than tissue remodeling.<br/><br> <br/><br> This thesis was aimed to characterize myofibroblast phenotypes and to elucidate the origin of these cells in airway remodeling, focused on tissue mainly derived from patients with mild asthma. To identify potential markers involved in disease mechanisms, methods used to study proteoglycan profiles and protein expression pattern (Proteomics) have been utilized. These topics, including the basics of airway remodeling, will be reviewed in the following sections.}}, author = {{Larsen, Kristoffer}}, isbn = {{91-85481-16-5}}, keywords = {{Medicine (human and vertebrates); Medicin (människa och djur); remodeling; General biomedical sciences; Biomedicinska vetenskaper (allmänt); fibrocytes; proteoglycans; Asthma; fibroblasts}}, language = {{eng}}, publisher = {{Department of Experimental Medical Science, Lund Univeristy}}, school = {{Lund University}}, title = {{Characterization of fibroblast phenotypes in human airway remodeling}}, year = {{2005}}, }