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Dopaminergic Differentiation of Human Embryonic Stem Cells on PA6-Derived Adipocytes.

Guloglu, Oktar LU and Larsen, Anna (2016) In Methods in Molecular Biology 1341. p.235-244
Abstract
Human embryonic stem cells (hESCs) are a promising source for cell replacement therapies. Parkinson's disease is one of the candidate diseases for the cell replacement therapy since the motor manifestations of the disease are associated with the loss of dopaminergic neurons in the substantia nigra pars compacta. Stromal cell-derived inducing activity (SDIA) is the most commonly used method for the dopaminergic differentiation of hESCs. This chapter describes a simple, reliable, and scalable dopaminergic induction method of hESCs using PA6-derived adipocytes. Coculturing hESCs with PA6-derived adipocytes markedly reduces the variable outcomes among experiments. Moreover, the colony differentiation step of this method can also be used for... (More)
Human embryonic stem cells (hESCs) are a promising source for cell replacement therapies. Parkinson's disease is one of the candidate diseases for the cell replacement therapy since the motor manifestations of the disease are associated with the loss of dopaminergic neurons in the substantia nigra pars compacta. Stromal cell-derived inducing activity (SDIA) is the most commonly used method for the dopaminergic differentiation of hESCs. This chapter describes a simple, reliable, and scalable dopaminergic induction method of hESCs using PA6-derived adipocytes. Coculturing hESCs with PA6-derived adipocytes markedly reduces the variable outcomes among experiments. Moreover, the colony differentiation step of this method can also be used for the dopaminergic induction of mouse embryonic stem cells and NTERA2 cells as well. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Methods in Molecular Biology
volume
1341
pages
235 - 244
publisher
Springer
external identifiers
  • pmid:25947418
  • scopus:84950336383
ISSN
1940-6029
DOI
10.1007/7651_2015_235
language
English
LU publication?
yes
id
527ffde8-40c5-4c77-8de4-b9fa08b5776e (old id 5456843)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25947418?dopt=Abstract
date added to LUP
2015-06-02 15:27:28
date last changed
2017-01-01 03:37:11
@article{527ffde8-40c5-4c77-8de4-b9fa08b5776e,
  abstract     = {Human embryonic stem cells (hESCs) are a promising source for cell replacement therapies. Parkinson's disease is one of the candidate diseases for the cell replacement therapy since the motor manifestations of the disease are associated with the loss of dopaminergic neurons in the substantia nigra pars compacta. Stromal cell-derived inducing activity (SDIA) is the most commonly used method for the dopaminergic differentiation of hESCs. This chapter describes a simple, reliable, and scalable dopaminergic induction method of hESCs using PA6-derived adipocytes. Coculturing hESCs with PA6-derived adipocytes markedly reduces the variable outcomes among experiments. Moreover, the colony differentiation step of this method can also be used for the dopaminergic induction of mouse embryonic stem cells and NTERA2 cells as well.},
  author       = {Guloglu, Oktar and Larsen, Anna},
  issn         = {1940-6029},
  language     = {eng},
  pages        = {235--244},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Dopaminergic Differentiation of Human Embryonic Stem Cells on PA6-Derived Adipocytes.},
  url          = {http://dx.doi.org/10.1007/7651_2015_235},
  volume       = {1341},
  year         = {2016},
}