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Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype.

Bordería, Antonio V; Isakov, Ofer; Moratorio, Gonzalo; Henningsson, Rasmus LU ; Agüera-González, Sonia; Organtini, Lindsey; Gnädig, Nina F; Blanc, Hervé; Alcover, Andrés and Hafenstein, Susan, et al. (2015) In PLoS Pathogens 11(5).
Abstract
Understanding how a pathogen colonizes and adapts to a new host environment is a primary aim in studying emerging infectious diseases. Adaptive mutations arise among the thousands of variants generated during RNA virus infection, and identifying these variants will shed light onto how changes in tropism and species jumps can occur. Here, we adapted Coxsackie virus B3 to a highly permissive and less permissive environment. Using deep sequencing and bioinformatics, we identified a multi-step adaptive process to adaptation involving residues in the receptor footprints that correlated with receptor availability and with increase in virus fitness in an environment-specific manner. We show that adaptation occurs by selection of a dominant... (More)
Understanding how a pathogen colonizes and adapts to a new host environment is a primary aim in studying emerging infectious diseases. Adaptive mutations arise among the thousands of variants generated during RNA virus infection, and identifying these variants will shed light onto how changes in tropism and species jumps can occur. Here, we adapted Coxsackie virus B3 to a highly permissive and less permissive environment. Using deep sequencing and bioinformatics, we identified a multi-step adaptive process to adaptation involving residues in the receptor footprints that correlated with receptor availability and with increase in virus fitness in an environment-specific manner. We show that adaptation occurs by selection of a dominant mutation followed by group selection of minority variants that together, confer the fitness increase observed in the population, rather than selection of a single dominant genotype. (Less)
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PLoS Pathogens
volume
11
issue
5
publisher
Public Library of Science
external identifiers
  • pmid:25941809
  • wos:000355269300014
  • scopus:84930338039
ISSN
1553-7366
DOI
10.1371/journal.ppat.1004838
language
English
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yes
id
51aa2442-ab39-4c1d-872f-1f4841b1e9e1 (old id 5456977)
date added to LUP
2015-06-17 12:21:33
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2017-11-05 03:19:56
@article{51aa2442-ab39-4c1d-872f-1f4841b1e9e1,
  abstract     = {Understanding how a pathogen colonizes and adapts to a new host environment is a primary aim in studying emerging infectious diseases. Adaptive mutations arise among the thousands of variants generated during RNA virus infection, and identifying these variants will shed light onto how changes in tropism and species jumps can occur. Here, we adapted Coxsackie virus B3 to a highly permissive and less permissive environment. Using deep sequencing and bioinformatics, we identified a multi-step adaptive process to adaptation involving residues in the receptor footprints that correlated with receptor availability and with increase in virus fitness in an environment-specific manner. We show that adaptation occurs by selection of a dominant mutation followed by group selection of minority variants that together, confer the fitness increase observed in the population, rather than selection of a single dominant genotype.},
  articleno    = {e1004838},
  author       = {Bordería, Antonio V and Isakov, Ofer and Moratorio, Gonzalo and Henningsson, Rasmus and Agüera-González, Sonia and Organtini, Lindsey and Gnädig, Nina F and Blanc, Hervé and Alcover, Andrés and Hafenstein, Susan and Fontes, Magnus and Shomron, Noam and Vignuzzi, Marco},
  issn         = {1553-7366},
  language     = {eng},
  number       = {5},
  publisher    = {Public Library of Science},
  series       = {PLoS Pathogens},
  title        = {Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype.},
  url          = {http://dx.doi.org/10.1371/journal.ppat.1004838},
  volume       = {11},
  year         = {2015},
}