Prognostic Significance of GPR55 mRNA Expression in Colon Cancer
(2022) In International Journal of Molecular Sciences 23(9).- Abstract
G protein-coupled receptor 55 (GPR55) probably plays a role in innate immunity and tumor immunosurveillance through its effect on immune cells, such as T cells and NK cells. In this study, the prognostic value of GPR55 in colon cancer (CC) was investigated. mRNA expression levels of GPR55 were determined in 382 regional lymph nodes of 121 CC patients with 12 years observation time after curative surgery. The same clinical material had previously been analyzed for expression levels of CEA, CXCL16, CXCL17, GPR35 V2/3 and LGR5 mRNAs. Clinical cutoffs of 0.1365 copies/18S rRNA unit for GPR55 and 0.1481 for the GPR55/CEA ratio were applied to differentiate between the high-and low-GPR55 expression groups. Kaplan–Meier survival analysis and... (More)
G protein-coupled receptor 55 (GPR55) probably plays a role in innate immunity and tumor immunosurveillance through its effect on immune cells, such as T cells and NK cells. In this study, the prognostic value of GPR55 in colon cancer (CC) was investigated. mRNA expression levels of GPR55 were determined in 382 regional lymph nodes of 121 CC patients with 12 years observation time after curative surgery. The same clinical material had previously been analyzed for expression levels of CEA, CXCL16, CXCL17, GPR35 V2/3 and LGR5 mRNAs. Clinical cutoffs of 0.1365 copies/18S rRNA unit for GPR55 and 0.1481 for the GPR55/CEA ratio were applied to differentiate between the high-and low-GPR55 expression groups. Kaplan–Meier survival analysis and Cox regression risk analysis were used to determine prognostic value. Improved discrimination between the two groups was achieved by combining GPR55 with CEA, CXCL16 or CXCL17 compared with GPR55 alone. The best result was obtained using the GPR55/CEA ratio, with an increased mean survival time of 14 and 33 months at 5 and 12 years observation time, respectively (p = 0.0003 and p = 0.003) for the high-GPR55/CEA group. The explanation for the observed improvement is most likely that GPR55 is a marker for T cells and B cells in lymph nodes, whereas CEA, CXCL16 and CXCL17, are markers for tumor cells of epithelial origin.
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- author
- Ismail, Hager Tarek H. ; Abdelmageed, Manar ; Lindmark, Gudrun LU ; Hammarström, Marie Louise ; Hammarström, Sten and Sitohy, Basel
- organization
- publishing date
- 2022-05-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- CEA, colon cancer, CXCL16, CXCL17, GPR55, prognosis, qRT-PCR, regional lymph nodes
- in
- International Journal of Molecular Sciences
- volume
- 23
- issue
- 9
- article number
- 4556
- publisher
- MDPI AG
- external identifiers
-
- scopus:85128397269
- pmid:35562947
- ISSN
- 1661-6596
- DOI
- 10.3390/ijms23094556
- language
- English
- LU publication?
- yes
- id
- 545acf0c-ab0f-4037-8b32-c1a5e3d94e09
- date added to LUP
- 2022-06-20 15:34:00
- date last changed
- 2024-06-11 00:24:24
@article{545acf0c-ab0f-4037-8b32-c1a5e3d94e09,
abstract = {{<p>G protein-coupled receptor 55 (GPR55) probably plays a role in innate immunity and tumor immunosurveillance through its effect on immune cells, such as T cells and NK cells. In this study, the prognostic value of GPR55 in colon cancer (CC) was investigated. mRNA expression levels of GPR55 were determined in 382 regional lymph nodes of 121 CC patients with 12 years observation time after curative surgery. The same clinical material had previously been analyzed for expression levels of CEA, CXCL16, CXCL17, GPR35 V2/3 and LGR5 mRNAs. Clinical cutoffs of 0.1365 copies/18S rRNA unit for GPR55 and 0.1481 for the GPR55/CEA ratio were applied to differentiate between the high-and low-GPR55 expression groups. Kaplan–Meier survival analysis and Cox regression risk analysis were used to determine prognostic value. Improved discrimination between the two groups was achieved by combining GPR55 with CEA, CXCL16 or CXCL17 compared with GPR55 alone. The best result was obtained using the GPR55/CEA ratio, with an increased mean survival time of 14 and 33 months at 5 and 12 years observation time, respectively (p = 0.0003 and p = 0.003) for the high-GPR55/CEA group. The explanation for the observed improvement is most likely that GPR55 is a marker for T cells and B cells in lymph nodes, whereas CEA, CXCL16 and CXCL17, are markers for tumor cells of epithelial origin.</p>}},
author = {{Ismail, Hager Tarek H. and Abdelmageed, Manar and Lindmark, Gudrun and Hammarström, Marie Louise and Hammarström, Sten and Sitohy, Basel}},
issn = {{1661-6596}},
keywords = {{CEA; colon cancer; CXCL16; CXCL17; GPR55; prognosis; qRT-PCR; regional lymph nodes}},
language = {{eng}},
month = {{05}},
number = {{9}},
publisher = {{MDPI AG}},
series = {{International Journal of Molecular Sciences}},
title = {{Prognostic Significance of GPR55 mRNA Expression in Colon Cancer}},
url = {{http://dx.doi.org/10.3390/ijms23094556}},
doi = {{10.3390/ijms23094556}},
volume = {{23}},
year = {{2022}},
}