Advanced

On acoustic particle and cell manipulation in microfluidic systems

Petersson, Filip LU (2007)
Abstract (Swedish)
Popular Abstract in Swedish

En kombination av laminära flöden i mikrofabricerade kanaler och akustiska krafter genererade i stående ultraljudsvågor erbjuder nya möjligheter för avancerad partikel- och cellhantering i både lab-on-a-chip-system och applikationer med högre kapacitetskrav. Akustisk partikelhantering kan användas exempelvis för att separera, tvätta, sortera, låsa fast och distribuera partiklar i mikrofluidiknätverk. Denna doktorsavhandling inleds med en översikt över de grundläggande ämnesområden som krävs för att framställa sådana system och för att förstå deras potentiella betydelse. Därefter beskrivs konstruktionen, tillverkningen och handhavandet av ett antal system baserade på den så kallade "Lundametoden".... (More)
Popular Abstract in Swedish

En kombination av laminära flöden i mikrofabricerade kanaler och akustiska krafter genererade i stående ultraljudsvågor erbjuder nya möjligheter för avancerad partikel- och cellhantering i både lab-on-a-chip-system och applikationer med högre kapacitetskrav. Akustisk partikelhantering kan användas exempelvis för att separera, tvätta, sortera, låsa fast och distribuera partiklar i mikrofluidiknätverk. Denna doktorsavhandling inleds med en översikt över de grundläggande ämnesområden som krävs för att framställa sådana system och för att förstå deras potentiella betydelse. Därefter beskrivs konstruktionen, tillverkningen och handhavandet av ett antal system baserade på den så kallade "Lundametoden". De utvecklade metoderna utgör en verktygslåda med generiska partikelhanteringsmetoder som kan kombineras eller användas var för sig för att hantera biologiska och icke-biologiska partiklar i vätskor. Verktygslådan används även för att lösa olika uppgifter inom blodhantering, t.ex. separation av fettpartiklar och andra förorenande substanser från röda blodkroppar och preparering av blodkomponenter. Resultaten visar att det kan vara möjligt att årligen rädda tusentals personer från hjärnskador orsakade av fettpartiklar, minska belastningen på de redan hårt ansträngda blodbankerna samt att göra nya metoder tillgängliga för rutinmässig hantering av blodkomponenter. Flera andra viktiga användningsområden där mikrometerstora partiklar hanteras rutinmässigt kan också identifieras. Akustisk partikelhantering i mikroskala är fortfarande i sin linda men baserat på de resultat som presenteras i denna avhandling och av andra forskare kan det förutspås att nya standarder för laboratorier och industrier mycket väl kan uppstå ur den forskning som för tillfället bedrivs inom fältet. (Less)
Abstract
The combination of laminar flows in microfabricated channels and acoustic forces induced in ultrasonic standing wave fields offers new possibilities for advanced particle and cell manipulation in lab-on-a-chip applications as well as in relatively high throughput applications. Acoustic particle manipulation systems can be used e.g. to separate, wash, sort, trap or distribute particles in microfluidic networks. This doctoral thesis starts by reviewing the fundamental fields of study needed to realize such systems and to understand their potential impact. Next, the design, fabrication, operation and performance of a number of systems, based on system design principle termed the "Lund method", are described. The developed methods comprise a... (More)
The combination of laminar flows in microfabricated channels and acoustic forces induced in ultrasonic standing wave fields offers new possibilities for advanced particle and cell manipulation in lab-on-a-chip applications as well as in relatively high throughput applications. Acoustic particle manipulation systems can be used e.g. to separate, wash, sort, trap or distribute particles in microfluidic networks. This doctoral thesis starts by reviewing the fundamental fields of study needed to realize such systems and to understand their potential impact. Next, the design, fabrication, operation and performance of a number of systems, based on system design principle termed the "Lund method", are described. The developed methods comprise a toolbox of generic particle handling methods that can be combined or used separately to handle biological and non-biological particles in liquid suspension. The toolbox is applied to solve various blood component handling tasks, e.g. separation of lipid particles and other contaminating substances from red blood cells and preparation of blood components. The results imply that it is possible to save thousands of people from brain damage caused by lipid particles each year, to reduce the strain on the blood banks significantly and to offer new methods for routine blood component handling. Several other important areas of application, where micrometer sized particles are routinely handled, can also be identified. Microscale acoustic particle manipulation technology is still in its infancy but, based on the findings presented in this thesis and by other researchers, it can be anticipated that new laboratory and industrial standards may very well emerge from the current research. (Less)
Please use this url to cite or link to this publication:
author
supervisor
opponent
  • Dr Hill, Martyn, Department of Mechanical Engineering, School of Engineering Sciences, University of Southampton, Uni
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Medicin (människa och djur), Natural science, Naturvetenskap, Technological sciences, Teknik, acoustic particle manipulation standing wave, acoustic radiation force, laminar flow, Medicine (human and vertebrates), microparticles, ultrasound, lab-on-a-chip, µ-TAS, separation, cells, microfabrication, microtechnology, microfluidics
publisher
Department of Electrical Measurements, Lund University
defense location
E:1406, E-building Ole Römers väg 3 Lund University Faculty of Engineering
defense date
2007-05-11 10:15
external identifiers
  • other:ISRN:LUTEDX/TEEM - - 1085 - - SE
ISSN
0346-6221
ISBN
978-91-628-7144-4
language
English
LU publication?
yes
id
ef897470-6347-4925-87cb-156825a0a9bd (old id 548511)
date added to LUP
2007-09-10 14:14:35
date last changed
2016-09-19 08:44:52
@phdthesis{ef897470-6347-4925-87cb-156825a0a9bd,
  abstract     = {The combination of laminar flows in microfabricated channels and acoustic forces induced in ultrasonic standing wave fields offers new possibilities for advanced particle and cell manipulation in lab-on-a-chip applications as well as in relatively high throughput applications. Acoustic particle manipulation systems can be used e.g. to separate, wash, sort, trap or distribute particles in microfluidic networks. This doctoral thesis starts by reviewing the fundamental fields of study needed to realize such systems and to understand their potential impact. Next, the design, fabrication, operation and performance of a number of systems, based on system design principle termed the "Lund method", are described. The developed methods comprise a toolbox of generic particle handling methods that can be combined or used separately to handle biological and non-biological particles in liquid suspension. The toolbox is applied to solve various blood component handling tasks, e.g. separation of lipid particles and other contaminating substances from red blood cells and preparation of blood components. The results imply that it is possible to save thousands of people from brain damage caused by lipid particles each year, to reduce the strain on the blood banks significantly and to offer new methods for routine blood component handling. Several other important areas of application, where micrometer sized particles are routinely handled, can also be identified. Microscale acoustic particle manipulation technology is still in its infancy but, based on the findings presented in this thesis and by other researchers, it can be anticipated that new laboratory and industrial standards may very well emerge from the current research.},
  author       = {Petersson, Filip},
  isbn         = {978-91-628-7144-4},
  issn         = {0346-6221},
  keyword      = {Medicin (människa och djur),Natural science,Naturvetenskap,Technological sciences,Teknik,acoustic particle manipulation standing wave,acoustic radiation force,laminar flow,Medicine (human and vertebrates),microparticles,ultrasound,lab-on-a-chip,µ-TAS,separation,cells,microfabrication,microtechnology,microfluidics},
  language     = {eng},
  publisher    = {Department of Electrical Measurements, Lund University},
  school       = {Lund University},
  title        = {On acoustic particle and cell manipulation in microfluidic systems},
  year         = {2007},
}