Smad signaling in hematopoietic stem cell biology

Abstract

Hematopoietic stem cells (HSCs) are primitive tissue-specific somatic stem cells, responsible for the maintenance and replenishment of the bone marrow (BM) and subsequently the entire blood system. The never-ending ability to differentiate into all the mature hematopoietic lineages makes these HSCs attractive candidates for use in future regenerative medicine. HSCs are balanced between different fate options through a fine-tuned orchestra of regulatory factors. The large superfamily of multifunctional growth factors known as the transforming growth factor 's (TGF-?s) has been suggested to play a role in this regulation. Despite acting through a seemingly simple signaling circuit, known as the Smad pathway, members of the TGF-? superfamily have various cellular effects depending on context. Several attempts have been made to link Smad signaling and HSC biology but the complexity of this system and its critical role for embryonic development have limited these studies to cell cultures. In this thesis, we have completely disrupted Smad signaling in adult HSCs in vivo by overexpressing the inhibitory Smad7 and by eliminating the common Smad4, using a conditional knockout model. The former approach prevents activation of R-Smads but leaves Smad4 intact whereas the latter model eliminates the physiological function of Smad4, the key downstream molecule where all Smad pathways converge. Additionally, we sought to identify gene targets for TGF-? by means of gene expression profiling analysis on activin receptor-like kinase (Alk) 5-/- fibroblast cell lines.



Smad7 overexpressing HSCs could long-term reconstitute the hematopoietic system of lethally irradiated recipients and displayed normal lineage distribution. However, following secondary transplantation, Smad7 overexpressing cells had a significantly increased regenerative capacity as compared to control transduced cells. In contrast, competitive repopulation capacity of Smad4-/- HSCs was significantly impaired and Smad4 null cells were completely out-competed in secondary hosts. Intriguingly, enforced expression of Smad7 in Smad4 null HSCs failed to expand HSCs in vivo, suggesting that the Smad7 HSC expansion phenotype is dependent on physiological concentrations of Smad4 and implicating that Smad4 is involved in relaying self-renewal signals from additional regulatory circuits. Moreover, gene expression profiling of murine embryonic fibroblasts, deficient in the TGF-? receptor revealed that TGF-? signaling occurs exclusively through Alk5 in these cells. Furthermore, we obtained a database of 369 differentially expressed genes which will be used to develop techniques for further studies of Smad signaling and cross-talk in HSCs. Taken together, our results have not only confirmed conventional theories regarding the role of TGF-? in HSC biology but also opened new exciting questions concerning the complexity and context dependency of TGF-? superfamily signaling, challenging our understanding of the Smad circuit in general.

• author: Karlsson, Göran
• supervisor: Stefan Karlsson; Markus Ringnér
• opponent: Professor Dzierzak, Elaine, Department of Cell Biology and Genetics, Erasmus University Medical Center, Rotterdam, The Netherlan
• organization: Division of Molecular Medicine and Gene Therapy
• publishing date: 2007
• type: Thesis
• publication status: published
• subject: Hematology
• keywords: Smad7, Smad4, microarray, retroviral-mediated gene expression, conditional knockout, transforming growth factor beta, smad signaling, hematopoiesis, hematopoietic stem cells, Alk5, Medicine (human and vertebrates), Medicin (människa och djur)
• pages: 114 pages
• defense location: Segerfalksalen, Wallenberg Neurocenter, Sölvegatan 17
• defense date: 2007-09-22 09:00:00
• ISBN: 978-91-85897-02-5
• language: English
• LU publication?: yes
• additional info: G Karlsson, U Blank, JL Moody, M Ehinger, S Singbrant, C-X Deng and S Karlsson. 2007. Smad4 is critical for hematopoietic stem cell self-renewal. Journal of Experimental Medicine, vol 204 pp 467-474.; U Blank, G Karlsson, JL Moody, T Utsugisawa, M Magnusson, S Singbrant, J Larsson and S Karlsson. 2006. Smad7 promotes self-renewal of hematopoietic stem cells in vivo. Blood, vol 108 pp 4246-4254.; G Karlsson, Y Liu, J Larsson, M-J Goumans, J-S Lee, S S Thorgeirsson, M Ringnér and S Karlsson. 2005. Gene expression profiling demonstrates that TGF-beta1 signals exclusively through receptor complexes involving Alk5 and identifies targets of TGF-beta signaling. Physiological Genomics, vol 21 pp 396-403.
• id: 6be90f94-706b-4bc2-9af8-3d995c9aa06f (old id 549004)
• date added to LUP: 2016-04-01 16:36:24
• date last changed: 2022-05-04 08:04:25